AB201455

Anti-NABC1 antibody [EPR15441(2)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal NABC1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AIBC1, NABC1, BCAS1, Breast carcinoma-amplified sequence 1, Amplified and overexpressed in breast cancer, Novel amplified in breast cancer 1

2 Images

Supplier Data

Western blot - Anti-NABC1 antibody [EPR15441(2)] (AB201455)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature PMID : 14567997. Please see page 6. The band observed at 120 kDa may represent a homodimer of NABC1, this information is based on SwissProt (Section : Subunit Structure).

All lanes:

Western blot - Anti-NABC1 antibody [EPR15441(2)] (ab201455) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 120 kDa,62 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-NABC1 antibody [EPR15441(2)] (AB201455)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NABC1 antibody [EPR15441(2)] (ab201455) at 1/5000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Lane 2:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 4:

SW480 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 120 kDa,62 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15441(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NABC1 also known as New ABC Transporter 1 is an ATP-binding cassette transporter protein with a molecular mass of around 160 kDa. Mechanically NABC1 functions as a transporter utilizing the energy from ATP hydrolysis to move lipids across cellular membranes. This protein is widely expressed in many tissues particularly in liver kidneys and intestines. Its transport activity plays a central role in maintaining cellular lipid homeostasis.

Biological function summary

NABC1 influences lipid metabolism by mediating the efflux of cholesterol and phospholipids from cells to lipid-poor apolipoproteins. It is not part of a larger protein complex but interacts dynamically with extracellular and membrane-associated proteins to facilitate lipid transport. These interactions are important for preventing lipid accumulation within cells which is essential for cellular health and normal functioning.

Pathways

NABC1 participates in the reverse cholesterol transport pathway and is integral to the regulation of cholesterol levels. It works closely with proteins such as apolipoprotein A-I to facilitate cholesterol removal from peripheral tissues thereby contributing to the overall cholesterol homeostasis. This pathway helps to prevent the buildup of excess cholesterol and protects against atherosclerosis.

Abnormal NABC1 activity links to conditions like Tangier disease and familial HDL deficiency where lipid transport becomes impaired. Mutations in NABC1 lead to reduced or absent transporter activity resulting in very low levels of HDL cholesterol in the blood. This defect causes cholesterol to accumulate in tissues causing complications associated with these disorders. NABC1's role in these conditions highlights its interaction with other proteins involved in lipid metabolism and cholesterol transport.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Required for myelination.

See full target information BCAS1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:438-448 PubMed39984653

2025

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis K Jeppesen,Zachary C Sanchez,Noah M Kelley,James B Hayes,Jessica Ambroise,Emma N Koory,Evan Krystofiak,Nilay Taneja,Qin Zhang,Matthew M Dungan,Olivia L Perkins,Matthew J Tyska,Ela W Knapik,Kevin M Dean,Amanda C Doran,Robert J Coffey,Dylan T Burnette

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NABC1 antibody [EPR15441(2)]

Western blot - Anti-NABC1 antibody [EPR15441(2)] (AB201455)

Western blot - Anti-NABC1 antibody [EPR15441(2)] (AB201455)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise