AB251343

Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NABC1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AIBC1, NABC1, BCAS1, Breast carcinoma-amplified sequence 1, Amplified and overexpressed in breast cancer, Novel amplified in breast cancer 1

2 Images

Supplier Data

Western blot - Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (AB251343)

This data was developed using ab201455, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature PMID : 14567997. Please see page 6. The band observed at 120 kDa may represent a homodimer of NABC1, this information is based on SwissProt (Section : Subunit Structure).

All lanes:

Western blot - Anti-NABC1 antibody [EPR15441(2)] (<a href='/en-us/products/primary-antibodies/nabc1-antibody-epr154412-ab201455'>ab201455</a>) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 120 kDa,62 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (AB251343)

This data was developed using ab201455, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NABC1 antibody [EPR15441(2)] (<a href='/en-us/products/primary-antibodies/nabc1-antibody-epr154412-ab201455'>ab201455</a>) at 1/5000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Lane 2:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 4:

SW480 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 120 kDa,62 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15441(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab251343 is the carrier-free version of ab201455.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NABC1 also known as New ABC Transporter 1 is an ATP-binding cassette transporter protein with a molecular mass of around 160 kDa. Mechanically NABC1 functions as a transporter utilizing the energy from ATP hydrolysis to move lipids across cellular membranes. This protein is widely expressed in many tissues particularly in liver kidneys and intestines. Its transport activity plays a central role in maintaining cellular lipid homeostasis.

Biological function summary

NABC1 influences lipid metabolism by mediating the efflux of cholesterol and phospholipids from cells to lipid-poor apolipoproteins. It is not part of a larger protein complex but interacts dynamically with extracellular and membrane-associated proteins to facilitate lipid transport. These interactions are important for preventing lipid accumulation within cells which is essential for cellular health and normal functioning.

Pathways

NABC1 participates in the reverse cholesterol transport pathway and is integral to the regulation of cholesterol levels. It works closely with proteins such as apolipoprotein A-I to facilitate cholesterol removal from peripheral tissues thereby contributing to the overall cholesterol homeostasis. This pathway helps to prevent the buildup of excess cholesterol and protects against atherosclerosis.

Abnormal NABC1 activity links to conditions like Tangier disease and familial HDL deficiency where lipid transport becomes impaired. Mutations in NABC1 lead to reduced or absent transporter activity resulting in very low levels of HDL cholesterol in the blood. This defect causes cholesterol to accumulate in tissues causing complications associated with these disorders. NABC1's role in these conditions highlights its interaction with other proteins involved in lipid metabolism and cholesterol transport.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for myelination.

See full target information BCAS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free

Western blot - Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (AB251343)

Western blot - Anti-NABC1 antibody [EPR15441(2)] - BSA and Azide free (AB251343)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise