Mouse Monoclonal Nac1 antibody. Suitable for Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NACC1.
Images
![Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093), expandable thumbnail](https://content.abcam.com/products/images/nac1-antibody-pcrp-nacc1-1a8-ab277093--flow-cytometry-img63732.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093)")
![Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093), expandable thumbnail](https://content.abcam.com/products/images/nac1-antibody-pcrp-nacc1-1a8-ab277093--protein-array-img1990.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NACC1. Database link Q96RE7
Reactivity data
Select an application| Flow Cyt | Protein Array | |
|---|---|---|
| Human | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Alternative names
BTBD14B, NAC1, NACC1, Nucleus accumbens-associated protein 1, NAC-1, BTB/POZ domain-containing protein 14B
Recommended products
Mouse Monoclonal Nac1 antibody. Suitable for Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NACC1.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NACC1. Database link Q96RE7
- Clone number
- PCRP-NACC1-1A8
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nac1 also known by other names like NACC1 is a transcriptional co-repressor involved in various cellular processes. With a molecular mass of approximately 63 kDa Nac1 is highly expressed in stem cells and several types of cancer cells. It functions by regulating the expression of target genes which influence cell survival and proliferation. In most cells the location of Nac1 is predominantly within the nucleus where it binds to DNA and interacts with other transcription factors.
- Biological function summary
The Nac1 protein plays a significant role in embryonic development and maintaining cell pluripotency. It forms a complex with several chromatin remodeling proteins assisting in the regulation of gene transcription. This involvement suggests its function is important in early developmental stages and in cells that require extensive plasticity. Besides Nac1 affects the cellular response to external stress stimuli contributing to cell survival under adverse conditions.
- Pathways
The Nac1 protein integrates into the Hedgehog signaling pathway and the ubiquitin-proteasome pathway. In the Hedgehog pathway Nac1 influences gene regulation that is essential for cellular differentiation and tissue development. In the ubiquitin-proteasome pathway Nac1 interacts with proteins such as Skp1 and Cullin1 which are part of the SCF complex to modulate protein degradation.
- Associated diseases and disorders
Nac1 has been linked with ovarian cancer and drug addiction. In ovarian cancer aberrant expression of Nac1 correlates with tumor growth and chemoresistance often involving interactions with the FAK protein. In drug addiction changes in the expression of Nac1 in the brain have been associated with the molecular adaptations to chronic drug exposure directly impacting the protein CREB which also plays a critical role in addiction pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093), expandable thumbnail](https://content.abcam.com/products/images/nac1-antibody-pcrp-nacc1-1a8-ab277093--flow-cytometry-img63732.jpg "Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)")
Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Nac1 using ab277093 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
![Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093), expandable thumbnail](https://content.abcam.com/products/images/nac1-antibody-pcrp-nacc1-1a8-ab277093--protein-array-img1990.jpg "Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)")
Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277093.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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Product protocols
- Visit the general protocols
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