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AB277093

Anti-Nac1 antibody [PCRP-NACC1-1A8]

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(1 Publication)

Mouse Monoclonal Nac1 antibody. Suitable for Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NACC1.

View Alternative Names

BTBD14B, NAC1, NACC1, Nucleus accumbens-associated protein 1, NAC-1, BTB/POZ domain-containing protein 14B

2 Images
Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093)

Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Nac1 using ab277093 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093)
  • Protein Array

Unknown

Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (AB277093)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277093.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PCRP-NACC1-1A8

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, Protein Array

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NACC1.

Q96RE7

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nac1 also known by other names like NACC1 is a transcriptional co-repressor involved in various cellular processes. With a molecular mass of approximately 63 kDa Nac1 is highly expressed in stem cells and several types of cancer cells. It functions by regulating the expression of target genes which influence cell survival and proliferation. In most cells the location of Nac1 is predominantly within the nucleus where it binds to DNA and interacts with other transcription factors.
Biological function summary

The Nac1 protein plays a significant role in embryonic development and maintaining cell pluripotency. It forms a complex with several chromatin remodeling proteins assisting in the regulation of gene transcription. This involvement suggests its function is important in early developmental stages and in cells that require extensive plasticity. Besides Nac1 affects the cellular response to external stress stimuli contributing to cell survival under adverse conditions.

Pathways

The Nac1 protein integrates into the Hedgehog signaling pathway and the ubiquitin-proteasome pathway. In the Hedgehog pathway Nac1 influences gene regulation that is essential for cellular differentiation and tissue development. In the ubiquitin-proteasome pathway Nac1 interacts with proteins such as Skp1 and Cullin1 which are part of the SCF complex to modulate protein degradation.

Nac1 has been linked with ovarian cancer and drug addiction. In ovarian cancer aberrant expression of Nac1 correlates with tumor growth and chemoresistance often involving interactions with the FAK protein. In drug addiction changes in the expression of Nac1 in the brain have been associated with the molecular adaptations to chronic drug exposure directly impacting the protein CREB which also plays a critical role in addiction pathways.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
See full target information NACC1

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 34:102234 PubMed40893347

2025

Cationic microbubble loading hSIRT3 and hTIMP3 optimize cardiac-targeted delivery and myocardial protection in the porcine MI/R model.

Applications

Unspecified application

Species

Unspecified reactive species

Peian Cai,Kegong Chen,Xionghai Qin,Xingpei Jiang,Xuan Jiao,Kexun Liu,Erliang Guo,Zipeng Li,Xianxin Qiu,Chang Liu,Lu Sun,Junbo Chuai,Jie Wu,Wei Chen,Hai Tian
View all publications

Product promise

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