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AB221950

Anti-NACA1 antibody

4

(2 Reviews)

|

(1 Publication)

Rabbit Polyclonal NACA1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HSD48, NACA, Nascent polypeptide-associated complex subunit alpha, NAC-alpha, Alpha-NAC

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-NACA1 antibody (AB221950)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NACA1 antibody (AB221950)

PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for NACA1 (green) using ab221950 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NACA1 also known as Nascent Polypeptide-Associated Complex Alpha-1 is a vital protein in cells with a molecular mass of around 21 kDa. It plays a big role as a transcription factor and coordinates the synthesis and proper folding of nascent polypeptides. This protein shows high expression in various tissues including muscle brain and liver. As a multifunctional player NACA1 assists in safeguarding polypeptides emerging during protein synthesis ensuring they achieve correct conformation and function.
Biological function summary

NACA1 modulates transcriptional activity interacting with particular sequences in DNA to affect gene expression. It forms a part of the nascent polypeptide-associated complex (NAC) that helps modulate developing polypeptides and ribosome-associated processes. By working in tandem with other proteins NACA1 not only contributes to protein folding but also aids in the regulation of stress responses. This highlights its importance in ensuring proteins function correctly under various cellular conditions.

Pathways

NACA1 engages in the regulation of protein homeostasis and gene expression pathways. It is involved in the unfolded protein response (UPR) and interacts with other ribosome-associated proteins. NACA1 plays a role in stress-response pathways ensuring proteins are correctly processed and folded under stress conditions. It may interact with other proteins like Hsp70 and ribosomal proteins to enhance protein quality control ensuring normal cellular function.

NACA1's dysregulation relates to conditions such as myopathies and cancer. Altered expression or function can disrupt normal stress response pathways resulting in muscle weakness or tumor progression. Researchers have noted its potential link with BRCA1-associated breast cancer and impacts seen in muscular diseases suggesting it may influence disease progression through its interaction with genes and proteins central to these disorders. Understanding NACA1’s role can support the development of new therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
See full target information NACA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 14:e1680 PubMed38769668

2024

Targeting c-Myc transactivation by LMNA inhibits tRNA processing essential for malate-aspartate shuttle and tumour progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqun Wang,Mei Hong,Yang Cheng,Xiaojing Wang,Dan Li,Guo Chen,Banghe Bao,Jiyu Song,Xinyi Du,Chunhui Yang,Liduan Zheng,Qiangsong Tong
View all publications
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Product promise

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