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AB127942

Anti-NADase antibody

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(6 Publications)

Rabbit Polyclonal NADase antibody. Suitable for WB, IP, FuncS (Neut/Block), Neut and reacts with Streptococcus sp. 'group A', Streptococcus sp. 'group C' samples. Cited in 6 publications.
1 Images
Western blot - Anti-NADase antibody (AB127942)
  • WB

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Western blot - Anti-NADase antibody (AB127942)

All lanes:

Western blot - Anti-NADase antibody (ab127942) at 1/2000 dilution

Lane 1:

Culture supernatant of group A streptococcus

Lane 2:

Culture supernatant of group C streptococcus

Lane 3:

Culture supernatant only (negative control)

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Streptococcus sp. 'group C', Streptococcus sp. 'group A'

Applications

Neut, FuncS (Neut/Block), WB, IP

applications

Specificity

ab127942 reacts with NADase of Group A, C,and G origins

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "Neut" : {"fullname" : "Neutralising", "shortname":"Neut"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Streptococcus sp. 'group A'": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "predicted", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "Neut-species-checked": "guaranteed", "Neut-species-dilution-info": "", "Neut-species-notes": "<p></p>" }, "Streptococcus sp. 'group C'": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p></p>", "Neut-species-checked": "predicted", "Neut-species-dilution-info": "", "Neut-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.09% Sodium azide Constituents: 99% Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NADase also known as Nicotinamide Adenine Dinucleotide glycohydrolase is an enzyme with a major role in catalyzing the hydrolysis of NAD+ into nicotinamide and ADP-ribose. This enzyme has an approximate mass of about 30-40 kDa although exact mass can vary depending on the isoform and organism. You can commonly find NADase expressed in various tissues including immune cells and neuronal tissues reflecting its broad functional significance.
Biological function summary

The enzyme facilitates cellular signaling and metabolic regulation by modulating the availability of NAD+ a critical substrate and cofactor in numerous metabolic pathways. NADase often functions as part of a larger protein complex participating in the regulation of calcium signaling and apoptosis. It also plays an important role in maintaining cellular homeostasis influencing processes like energy metabolism and DNA repair.

Pathways

NADase contributes significantly to NAD+ metabolism and calcium signaling pathways. It interacts with other enzymes such as Sirtuins which use NAD+ for deacetylation reactions involved in gene expression regulation and aging. In these pathways NADase influences the cellular response to metabolic stress and helps maintain the balance between energy production and consumption.

NADase has links to neurodegenerative diseases and certain types of cancer. Alterations in NADase activity can affect the progression of Alzheimer’s disease where disturbed NAD+ metabolism plays a role in neuronal dysfunction. In some cancers the enzyme's dysregulation can alter cellular metabolism enhancing tumor survival. NADase connects to these diseases through interactions with proteins like PARP-1 which are involved in DNA damage repair and apoptosis regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information NADase
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 40: PubMed32285913

2020

Dexmedetomidine alleviated neuropathic pain in dorsal root ganglion neurons by inhibition of anaerobic glycolysis activity and enhancement of ROS tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Peibin Liu,Tufeng Chen,Fang Tan,Jingling Tian,Lei Zheng,Yingqing Deng,Jiaxin Chen,Xinjin Chi

Infection and immunity 84:2086-2093 PubMed27141081

2016

Intergenic Variable-Number Tandem-Repeat Polymorphism Upstream of rocA Alters Toxin Production and Enhances Virulence in Streptococcus pyogenes.

Applications

Unspecified application

Species

Unspecified reactive species

Luchang Zhu,Randall J Olsen,Nicola Horstmann,Samuel A Shelburne,Jia Fan,Ye Hu,James M Musser

The Journal of clinical investigation 125:3545-59 PubMed26258415

2015

A molecular trigger for intercontinental epidemics of group A Streptococcus.

Applications

Unspecified application

Species

Unspecified reactive species

Luchang Zhu,Randall J Olsen,Waleed Nasser,Stephen B Beres,Jaana Vuopio,Karl G Kristinsson,Magnus Gottfredsson,Adeline R Porter,Frank R DeLeo,James M Musser

Antimicrobial agents and chemotherapy 54:98-102 PubMed19805566

2009

Clindamycin-induced CovS-mediated regulation of the production of virulent exoproteins streptolysin O, NAD glycohydrolase, and streptokinase in Streptococcus pyogenes.

Applications

Unspecified application

Species

Unspecified reactive species

Masaaki Minami,Takuya Kamimura,Masanori Isaka,Ichiro Tatsuno,Michio Ohta,Tadao Hasegawa

The Journal of biological chemistry 281:9181-9 PubMed16380378

2005

Genetic and biochemical properties of streptococcal NAD-glycohydrolase inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Hisashi Kimoto,Yutaka Fujii,Satoko Hirano,Yoshifumi Yokota,Akira Taketo

The Journal of infectious diseases 182:1117-28 PubMed10979908

2000

Molecular epidemiology of nga and NAD glycohydrolase/ADP-ribosyltransferase activity among Streptococcus pyogenes causing streptococcal toxic shock syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

D L Stevens,D B Salmi,E R McIndoo,A E Bryant
View all publications
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