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AB219821

Anti-NADH2 antibody [9E12-1B3] - N-terminal

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(1 Publication)

Mouse Monoclonal NU2M antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MT-ND2.

View Alternative Names

MTND2, NADH2, ND2, MT-ND2, NADH-ubiquinone oxidoreductase chain 2, NADH dehydrogenase subunit 2

1 Images
Western blot - Anti-NADH2 antibody [9E12-1B3] - N-terminal (AB219821)
  • WB

Supplier Data

Western blot - Anti-NADH2 antibody [9E12-1B3] - N-terminal (AB219821)

Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

All lanes:

Western blot - Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821) at 2 µg/mL

Lane 1:

Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn) at 10 µg

Lane 2:

Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide at 10 µg

Secondary

All lanes:

HRP-labeled Goat-anti-mouse IgG

Predicted band size: 39 kDa

Observed band size: 32 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9E12-1B3

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MT-ND2. The exact immunogen used to generate this antibody is proprietary information.

P03891

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>Western blot using whole cell extracts is not recommended.</p>" } } }
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Product details

Western blot advice:

Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

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Properties and storage information

Form
Liquid
Purification notes
Purified from hybridoma cell culture supernatant by biochemical fractionation from serum-free medium.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 0.87% Sodium chloride, 0.36% HEPES
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NADH2 also known as NADH dehydrogenase 2 is an important enzyme in cellular respiration. It is often referred to as 9e12 in some contexts. It plays a mechanical role in the electron transport chain functioning within the inner mitochondrial membrane where it catalyzes the transfer of electrons from NADH to ubiquinone. This activity contributes to the proton gradient necessary for ATP synthesis. NADH2 has a mass that varies depending on the isoform but typically around 35 kDa. The enzyme is expressed in various tissues notably where high energy demand exists such as in muscle tissues and the liver.
Biological function summary

The enzyme engages in oxidizing NADH thereby producing NAD+ and contributing electrons to the respiratory chain. NADH2 combines with other subunits as part of a larger multi-subunit complex called Complex I in mitochondria. This complex represents a pivotal element in creating the electrochemical gradient used by ATP synthase to produce ATP the main energy currency of the cell. Disturbances in Complex I activity can result in impaired energy production.

Pathways

NADH2 forms an integral component of oxidative phosphorylation and the electron transport chain. It is closely associated with pathways that manage oxidative respiration and energy metabolism within the cell. Important related proteins include cytochrome c and ATP synthase which work downstream of NADH2 in the electron transport chain. Any disruptions in these pathways may significantly influence cellular energy metabolism and overall cellular health.

Malfunctions or deficiencies in NADH2 are linked to mitochondrial diseases notably Leber's hereditary optic neuropathy (LHON) and neurodegenerative diseases such as Parkinson's disease. These connections highlight its critical role in maintaining mitochondrial function and energy production. In the context of these disorders NADH2 often interacts aberrantly with proteins such as complex IV components and other mitochondrially encoded proteins which may exacerbate symptoms through disrupted energy metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 16996290). Essential for the catalytic activity and assembly of complex I (PubMed : 16996290).
See full target information MT-ND2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 19:174 PubMed33902636

2021

Identification of key methylation differentially expressed genes in posterior fossa ependymoma based on epigenomic and transcriptome analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Guanyi Wang,Yibin Jia,Yuqin Ye,Enming Kang,Huijun Chen,Jiayou Wang,Xiaosheng He
View all publications
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