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AB241424

Anti-NADK antibody

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(1 Publication)

Rabbit Polyclonal NADK antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NADK aa 1-100.

View Alternative Names

NAD kinase, Poly(P)/ATP NAD kinase, NADK

2 Images
Immunoprecipitation - Anti-NADK antibody (AB241424)
  • IP

Supplier Data

Immunoprecipitation - Anti-NADK antibody (AB241424)

NADK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241424 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241424 at 1 μg/ml.

Lane 1 : ab241424 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-NADK antibody (ab241424)

Predicted band size: 49 kDa

false

Western blot - Anti-NADK antibody (AB241424)
  • WB

Supplier Data

Western blot - Anti-NADK antibody (AB241424)

All lanes:

Western blot - Anti-NADK antibody (ab241424) at 1 µg/mL

Lanes 1 and 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NADK aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O95544

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to NADK immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAD kinase also known as NADK is a critical enzyme responsible for phosphorylating nicotinamide adenine dinucleotide (NAD+) to form NADP+. NADK has an approximate molecular weight of 50 kDa. Its expression is widespread but it is especially abundant in pancreatic and liver tissues. This enzyme converts NAD+ to NADP+ which is important for maintaining the balance of NADP+/NADPH within cells impacting several biochemical processes.
Biological function summary

The conversion role played by NADK is essential for maintaining cellular redox status and for biosynthetic reactions that require NADPH such as fatty acid synthesis and detoxifying reactive oxygen species. NADK functions independently without being part of larger protein complexes. It facilitates key cellular processes by ensuring adequate levels of NADPH a necessary cofactor in various anabolic reactions and cellular defense mechanisms.

Pathways

NADK prominently features in the pentose phosphate pathway which is important for the production of NADPH. It interacts with enzymes like glucose-6-phosphate dehydrogenase (G6PD) which is also involved in producing NADPH through this pathway. Additionally NADK plays a role in the citric acid cycle where its activity supports energy metabolism by maintaining cellular NADP+/NADPH ratios.

Altered NADK activity links to conditions such as cancer and diabetes. Its role in regulating redox state and metabolic pathways means that dysregulation can lead to oxidative stress contributing to the pathogenesis of several types of cancer. Disrupted glucose metabolism in diabetes associates G6PD and NADK since both are pivotal in maintaining NADPH levels highlighting the enzyme's connection with metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information NADK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

IUBMB life 74:1094-1114 PubMed36104956

2022

iTRAQ-based proteomic analysis of 17β-Estradiol-induced anti-proliferation and apoptosis in mouse thymic epithelial cells by disturbing ribosomal biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yameng Liu,Zilong Li,Dongguang Guo,Yongjiang Ma,Yugu Li
View all publications
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Product promise

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