Anti-NADPH oxidase 4 antibody [EPR28818-92]

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue staining NADPH oxidase 4 with ab319055 at a 1 : 500 (1.032 ug/ml) dilution, ab233837 anti-Hexokinase 1 used at 1 : 250 (4.36 ug/ml) dilution and ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1 : 4000 (0.132 ug/ml) dilution.

Panel A : merged staining of anti-NADPH oxidase 4 (green; Opal™520), anti-Hexokinase 1 (magenta; Opal™690) and anti-Angiotensin Converting Enzyme 1 (yellow; Opal™570) on rat kidney.
Panel B : anti-NADPH oxidase 4 staining distal tubules in rat kidney.
Panel C : anti-Hexokinase 1 staining distal tubules in rat kidney.
Panel D : anti-Angiotensin Converting Enzyme 1 staining proximal tubules in rat kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab319055, ab233837 and ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining NADPH oxidase 4 with ab319055 at a 1 : 500 (1.032 ug/ml) dilution, ab199975 anti-Aquaporin 2 used at 1 : 4000 (0.143 ug/ml) dilution and ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1 : 4000 (0.132 ug/ml) dilution.

Panel A : merged staining of anti-NADPH oxidase 4 (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Angiotensin Converting Enzyme 1 (yellow; Opal™570) on mouse kidney.
Panel B : anti-NADPH oxidase 4 staining distal tubules in mouse kidney.
Panel C : anti-Aquaporin 2 staining collecting tubules in mouse kidney.
Panel D : anti-Angiotensin Converting Enzyme 1 staining proximal tubules in mouse kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab319055, ab199975 and ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue staining NADPH oxidase 4 with ab319055 at a 1 : 500 (1.032 ug/ml) dilution, ab199975 anti-Aquaporin 2 used at 1 : 4000 (0.143 ug/ml) dilution and ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1 : 4000 (0.132 ug/ml) dilution.

Panel A : merged staining of anti-NADPH oxidase 4 (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Angiotensin Converting Enzyme 1 (yellow; Opal™570) on rat kidney.
Panel B : anti-NADPH oxidase 4 staining distal tubules in rat kidney.
Panel C : anti-Aquaporin 2 staining collecting tubules in rat kidney.
Panel D : anti-Angiotensin Converting Enzyme 1 staining proximal tubules in rat kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab319055, ab199975 and ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining NADPH oxidase 4 with ab319055 at a 1 : 500 (1.032 ug/ml) dilution, ab233837 anti-Hexokinase 1 used at 1 : 250 (4.36 ug/ml) dilution and ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1 : 4000 (0.132 ug/ml) dilution.

Panel A : merged staining of anti-NADPH oxidase 4 (green; Opal™520), anti-Hexokinase 1 (magenta; Opal™690) and anti-Angiotensin Converting Enzyme 1 (yellow; Opal™570) on mouse kidney.
Panel B : anti-NADPH oxidase 4 staining distal tubules in mouse kidney.
Panel C : anti-Hexokinase 1 staining distal tubules in mouse kidney.
Panel D : anti-Angiotensin Converting Enzyme 1 staining proximal tubules in mouse kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab319055, ab233837 and ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NADPH oxidase 4 with ab319055 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat kidney.
The section was incubated with ab319055 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling NADPH oxidase 4 with ab319055 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat cerebrum.
The section was incubated with ab319055 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling NADPH oxidase 4 with ab319055 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse kidney (PMID : 32236843).
The section was incubated with ab319055 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse NOX4 expression vector containing a his tag. (B) HEK-293T transfected with a mouse NOX1 expression vector containing a his tag. (C) HEK-293T transfected with a mouse NOX2 expression vector containing a his tag. (D) HEK-293T transfected with a mouse NOX3 expression vector containing a his tag. (E) HEK-293T cells transfected with empty vector containing a myc-His-tag. tissue labeling NADPH oxidase 4 with ab319055 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a mouse NOX4 expression vector containing a his tag, no staining on (B) HEK-293T transfected with a mouse NOX1 expression vector containing a his tag, (C) HEK-293T transfected with a mouse NOX2 expression vector containing a his tag, (D) HEK-293T transfected with a mouse NOX3 expression vector containing a his tag and (E) HEK-293T cells transfected with empty vector containing a myc-His-tag.
The section was incubated with ab319055 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling NADPH oxidase 4 with ab319055 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat liver.
The section was incubated with ab319055 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NADPH oxidase 4 with ab319055 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse cerebrum.
The section was incubated with ab319055 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [EPR28818-92] (AB319055)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling NADPH oxidase 4 with ab319055 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse liver.
The section was incubated with ab319055 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins