Rabbit Recombinant Monoclonal NADSYN1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--western-blot-img57533.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28227.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15060.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661)")
![Immunocytochemistry/ Immunofluorescence - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--immunocytochemistry-immunofluorescence-img9878.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661)")
![Flow Cytometry (Intracellular) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--flow-cytometry-intracellular-img79385.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (AB249661)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalyzes the final step of the nicotinamide adenine dinucleotide (NAD) de novo synthesis pathway, the ATP-dependent amidation of deamido-NAD using L-glutamine as a nitrogen source.
Alternative names
Glutamine-dependent NAD(+) synthetase, NAD(+) synthase [glutamine-hydrolyzing], NAD(+) synthetase, NADSYN1
Recommended products
Rabbit Recombinant Monoclonal NADSYN1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10611
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249661 is the carrier-free version of Anti-NADSYN1 antibody [EPR10611] ab171942.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NADSYN1 also known as NAD synthetase 1 catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinic acid adenine dinucleotide (NaAD) and ammonium. The protein plays a critical role in NAD biosynthesis. NADSYN1 has a molecular mass of approximately 66 kDa and it is mostly expressed in the liver kidney and brain. These sites of expression highlight its importance in various tissue types where energy metabolism and cell signaling are important.
- Biological function summary
NADSYN1 facilitates the production of NAD an important molecule in several metabolic processes. NAD is a coenzyme that is involved in redox reactions transferring electrons from one reaction to another. NADSYN1 functions as a monomer though it participates in larger biochemical pathways rather than forming a complex itself. Its activity ensures the availability of NAD which is necessary for efficient energy production and cellular functions.
- Pathways
Its activity impacts the central NAD biosynthesis pathway. Nicotinamide adenine dinucleotide is essential in glycolysis and the citric acid cycle which are important for energy production in cells. NADSYN1 activity influences these pathways and it maintains cellular energy homeostasis. Alongside its involvement in NAD biosynthesis it has indirect relations with proteins like NAMPT in the salvage pathway of NAD synthesis as both serve the function of replenishing cellular NAD levels.
- Associated diseases and disorders
Abnormalities in NADSYN1 function can lead to metabolic disorders including congenital NAD deficiency disorders. Such disruptions can impact energy metabolism and lead to neurological and developmental issues. The dysfunction of NADSYN1 linked with oxidative stress disorders relates to its role in maintaining NAD levels which manage cellular redox states. The protein is essential in preventing energy deficits and minimizing the risk of disorders related to impaired NAD metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--western-blot-img57533.jpg "Western blot - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)")
Western blot - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)
This data was developed using Anti-NADSYN1 antibody [EPR10611] ab171942, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NADSYN1 antibody [EPR10611] (Anti-NADSYN1 antibody [EPR10611] ab171942) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Predicted band size: 79 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28227.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)
This data was developed using Anti-NADSYN1 antibody [EPR10611] ab171942, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling NADSYN1 with Anti-NADSYN1 antibody [EPR10611] ab171942 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15060.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)
This data was developed using Anti-NADSYN1 antibody [EPR10611] ab171942, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NADSYN1 with Anti-NADSYN1 antibody [EPR10611] ab171942 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--immunocytochemistry-immunofluorescence-img9878.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)")
Immunocytochemistry/ Immunofluorescence - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)
This data was developed using Anti-NADSYN1 antibody [EPR10611] ab171942, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling NADSYN1 with Anti-NADSYN1 antibody [EPR10611] ab171942 at 1/50 dilution.
![Flow Cytometry (Intracellular) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661), expandable thumbnail](https://content.abcam.com/products/images/nadsyn1-antibody-epr10611-bsa-and-azide-free-ab249661--flow-cytometry-intracellular-img79385.jpg "Flow Cytometry (Intracellular) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)")
Flow Cytometry (Intracellular) - Anti-NADSYN1 antibody [EPR10611] - BSA and Azide free (ab249661)
This data was developed using Anti-NADSYN1 antibody [EPR10611] ab171942, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling NADSYN1 with Anti-NADSYN1 antibody [EPR10611] ab171942 at 1/100 dilution (red) ocompared with a Rabbit IgG (negative) (green).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com