AB122323

Anti-NAF1 antibody

Name: Anti-NAF1 antibody (ab122323) | Abcam
Brand: Abcam Limited
SKU: AB122323

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(2 Publications)

Rabbit Polyclonal NAF1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human H/ACA ribonucleoprotein complex non-core subunit NAF1 aa 200-350.

View Alternative Names

H/ACA ribonucleoprotein complex non-core subunit NAF1, hNAF1, NAF1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (AB122323)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (AB122323)

Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human esophagus with ab122323 at 1/100 dilution showing strong nuclear and cytoplasmic positivity in squamous epithelial cells

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human H/ACA ribonucleoprotein complex non-core subunit NAF1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q96HR8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAF1 also known as NHP2L1 is a protein involved in essential mechanical processes in the cell. It weighs approximately 83 kDa and mainly localizes to the nucleus specifically within Cajal bodies and nucleolus. The protein plays a role in the assembly and maturation of small nucleolar ribonucleoproteins (snoRNPs) which are important for RNA processing. NAF1 expression occurs across diverse tissues indicating its importance in general cellular function.

Biological function summary

NAF1 is a component of the H/ACA ribonucleoprotein complex. It participates in guiding the chemical modification of ribosomal and other small nuclear RNAs. NAF1 acts as a scaffold facilitating the attachment of small RNAs and interacting with other proteins like NOP10 and GAR1 within the snoRNP complex. This organization is essential for the stability and function of the complex which impacts RNA folding and maturation ultimately regulating protein synthesis.

Pathways

The involvement of NAF1 in RNA modification is critical in the RNA splicing and ribosome biogenesis pathways. It plays a role in the maturation of ribosomal RNA an important step in assembling functioning ribosomes. NAF1's activity indirectly influences the translation process with links to additional proteins such as dyskerin which also have essential functions in ribosome assembly and RNA stability.

Studies have connected NAF1 function to conditions like dyskeratosis congenita and certain cancers. The link to dyskeratosis congenita arises from its association with the stability and function of telomerase RNA a pathway shared with proteins such as dyskerin. In some cancers abnormalities in NAF1 expression or function have been implicated in tumor progression highlighting its potential role in maintaining genomic stability and cellular proliferation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.

See full target information H/ACA ribonucleoprotein complex non-core subunit NAF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:61 PubMed38229161

2024

Inhibition of soluble epoxide hydrolase enhances the dentin-pulp complex regeneration mediated by crosstalk between vascular endothelial cells and dental pulp stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lingwenyao Kong,Juanjuan Li,Yuwen Bai,Shaoyang Xu,Lin Zhang,Weixian Chen,Lu Gao,Fu Wang

Nucleic acids research 51:3357-3374 PubMed36869663

2023

Proteomic analyses reveal new features of the box H/ACA RNP biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Florence Schlotter,Salim Mérouani,Justine Flayac,Valentyne Kogey,Amani Issa,Maxime Dodré,Alexandra Huttin,Christiane Branlant,Edouard Bertrand,Stéphane Labialle,Franck Vandermoere,Céline Verheggen,Séverine Massenet

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NAF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (AB122323)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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