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AB249634

Anti-NAF1 antibody [EPR11516] - BSA and Azide free

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Rabbit Recombinant Monoclonal NAF1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

H/ACA ribonucleoprotein complex non-core subunit NAF1, hNAF1, NAF1

2 Images
Flow Cytometry (Intracellular) - Anti-NAF1 antibody [EPR11516] - BSA and Azide free (AB249634)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NAF1 antibody [EPR11516] - BSA and Azide free (AB249634)

This data was developed using ab171078, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling NAF1 with ab171078 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

Western blot - Anti-NAF1 antibody [EPR11516] - BSA and Azide free (AB249634)
  • WB

Supplier Data

Western blot - Anti-NAF1 antibody [EPR11516] - BSA and Azide free (AB249634)

This data was developed using ab171078, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NAF1 antibody [EPR11516] (<a href='/en-us/products/primary-antibodies/naf1-antibody-epr11516-ab171078'>ab171078</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

Namalwa cell lysate at 10 µg

Lane 5:

Caco-2 cell lysate at 10 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11516

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249634 is the carrier-free version of ab171078.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAF1 also known as NHP2L1 is a protein involved in essential mechanical processes in the cell. It weighs approximately 83 kDa and mainly localizes to the nucleus specifically within Cajal bodies and nucleolus. The protein plays a role in the assembly and maturation of small nucleolar ribonucleoproteins (snoRNPs) which are important for RNA processing. NAF1 expression occurs across diverse tissues indicating its importance in general cellular function.
Biological function summary

NAF1 is a component of the H/ACA ribonucleoprotein complex. It participates in guiding the chemical modification of ribosomal and other small nuclear RNAs. NAF1 acts as a scaffold facilitating the attachment of small RNAs and interacting with other proteins like NOP10 and GAR1 within the snoRNP complex. This organization is essential for the stability and function of the complex which impacts RNA folding and maturation ultimately regulating protein synthesis.

Pathways

The involvement of NAF1 in RNA modification is critical in the RNA splicing and ribosome biogenesis pathways. It plays a role in the maturation of ribosomal RNA an important step in assembling functioning ribosomes. NAF1's activity indirectly influences the translation process with links to additional proteins such as dyskerin which also have essential functions in ribosome assembly and RNA stability.

Studies have connected NAF1 function to conditions like dyskeratosis congenita and certain cancers. The link to dyskeratosis congenita arises from its association with the stability and function of telomerase RNA a pathway shared with proteins such as dyskerin. In some cancers abnormalities in NAF1 expression or function have been implicated in tumor progression highlighting its potential role in maintaining genomic stability and cellular proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
See full target information H/ACA ribonucleoprotein complex non-core subunit NAF1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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