AB95182

Anti-NAGK antibody

Name: Anti-NAGK antibody (ab95182) | Abcam
Brand: Abcam Limited
SKU: AB95182
Price: 520 USD
Availability: InStock

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Rabbit Polyclonal NAGK antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NAGK aa 50-100.

View Alternative Names

N-acetyl-D-glucosamine kinase, N-acetylglucosamine kinase, GlcNAc kinase, Muramyl dipeptide kinase, N-acetyl-D-mannosamine kinase, NAGK

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Immunoprecipitation - Anti-NAGK antibody (AB95182)

Detection of Human NAGK by Immunoprecipitation and Western Blot. Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded. ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2) NAGK was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3). Detection : Chemiluminescence with exposure times of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NAGK antibody (ab95182)

Predicted band size: 37 kDa

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Western blot - Anti-NAGK antibody (AB95182)

All lanes:

Western blot - Anti-NAGK antibody (ab95182) at 0.4 µg/mL

Lane 1:

Whole cell lysate from HeLa at 50 µg

Lane 2:

Whole cell lysate from HeLa at 15 µg

Lane 3:

Whole cell lysate from HeLa at 5 µg

Lane 4:

293T cell lysate at 50 µg

Predicted band size: 37 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NAGK aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UJ70

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab95182 was affinity purified using an epitope specific to NAGK immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Acetylglucosamine Kinase (NAGK) also known as GlcNAc kinase catalyzes the phosphorylation of N-acetylglucosamine to N-acetylglucosamine-6-phosphate. It has a molecular mass of approximately 37 kDa. NAGK expresses in various tissues prominently within the liver and pancreas indicating its involvement in metabolic functions related to these organs.

Biological function summary

NAGK plays a significant role in amino sugar and nucleotide sugar metabolism. It does not form part of a large protein complex but interacts with other metabolic enzymes to carry out its function. By converting N-acetylglucosamine to its phosphorylated form NAGK helps to maintain cellular energy balance and provides substrates necessary for glycosylation processes.

Pathways

The enzymatic activity of NAGK is essential in the hexosamine biosynthetic pathway. This pathway feeds into glycosylation processes necessary for protein modification and cellular communication. NAGK activity links to proteins such as UDP-N-acetylglucosamine pyrophosphorylase in these pathways highlighting its role in maintaining cellular function and integrity.

Alterations in NAGK function relate to metabolic syndromes and potential glycosylation disorders. Changes in NAGK could affect pathways involving N-linked glycosylation which plays a role in diseases like congenital disorders of glycosylation. Proteins such as glutamine fructose-6-phosphate amidotransferase may also interact with NAGK in the context of these diseases affecting broader metabolic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate (PubMed : 22692205). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway : although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (PubMed : 22692205). Also has N-acetylmannosamine (ManNAc) kinase activity (By similarity). Also involved in innate immunity by promoting detection of bacterial peptidoglycan by NOD2 : acts by catalyzing phosphorylation of muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, to generate 6-O-phospho-muramyl dipeptide, which acts as a direct ligand for NOD2 (PubMed : 36002575).

See full target information NAGK

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NAGK antibody

Immunoprecipitation - Anti-NAGK antibody (AB95182)

Western blot - Anti-NAGK antibody (AB95182)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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