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AB214671

Anti-NAGLU/NAG antibody [EPR20708]

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(7 Publications)

Rabbit Recombinant Monoclonal NAGLU/NAG antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

UFHSD1, NAGLU, Alpha-N-acetylglucosaminidase, N-acetyl-alpha-glucosaminidase, NAG

10 Images
Western blot - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • WB

Supplier Data

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-2,5-6 : 3 minutes; Lanes 3-4 : 30 seconds.

Lanes 1 - 4:

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/2000 dilution

Lanes 5 - 6:

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution

Lane 1:

Mouse colon lysate at 20 µg

Lane 2:

Rat testis lysate at 20 µg

Lane 3:

Mouse testis lysate at 20 µg

Lane 4:

Rat colon lysate at 20 µg

Lane 5:

Rat liver lysate at 10 µg

Lane 6:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human liver (PMID : 4291567 PMID : 8776591). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on rat kidney tubules (PMID : 4291567 PMID : 24244710). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human kidney tubules (PMID : 4291567 PMID : 24244710). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Immunofluorescent analysis of 100% methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse kidney tubules (PMID : 4291567 PMID : 24244710). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Immunofluorescent analysis of 100% methanol-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)
  • WB

Supplier Data

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 1 minute; Lanes 3-4 : 30 seconds.

Lanes 1 - 3:

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution

Lane 4:

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/5000 dilution

Lane 1:

Human placenta lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Lanes 3 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa

true

  • Carrier free

    Anti-NAGLU/NAG antibody [EPR20708] - BSA and Azide free

  • 578 PE

    PE Anti-NAGLU/NAG antibody [EPR20708]

  • HRP

    HRP Anti-NAGLU/NAG antibody [EPR20708]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-NAGLU antibody [EPR20708]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-NAGLU antibody [EPR20708]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-NAGLU antibody [EPR20708]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-NAGLU/NAG antibody [EPR20708]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-NAGLU antibody [EPR20708]

  • 660 APC

    APC Anti-NAGLU/NAG antibody [EPR20708]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-NAGLU/NAG antibody [EPR20708]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20708

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Additional bands observed in some rodent tissue lysates where NAGLU is not highly expressed.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Additional bands observed in some rodent tissue lysates where NAGLU is not highly expressed.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Additional bands observed in some rodent tissue lysates where NAGLU is not highly expressed.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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AB204705

N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric)

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAGLU/NAG also known as alpha-N-acetylglucosaminidase is an enzyme with a molecular weight of approximately 83 kDa. This enzyme localizes in the lysosome where cells express it across various tissues. NAGLU plays a significant role in the breakdown of heparan sulfate which is a glycosaminoglycan found on cell surfaces and in the extracellular matrix. Its enzymatic action involves the cleavage of terminal alpha-N-acetylglucosamine residues during lysosomal degradation.
Biological function summary

NAGLU facilitates the degradation of heparan sulfate in conjunction with other enzymes as part of a degradation complex within the lysosome. NAGLU's action is essential for maintaining the balance of heparan sulfate levels inside cells which is important for cellular health and function. Without the proper function of this enzyme heparan sulfate accumulates within lysosomes due to incomplete catabolism leading to cellular dysfunction and related pathologies.

Pathways

NAGLU functions critically in the glycosaminoglycan degradation pathway. This pathway involves several enzymes including iduronate-2-sulfatase which acts upstream of NAGLU. Together they ensure the breakdown of complex sugars such as heparan sulfate. NAGLU also participates in the larger lysosomal degradation pathway collaborating with other lysosomal enzymes to ensure proper breakdown and recycling of cellular materials.

Disruptions in NAGLU function are directly linked to mucopolysaccharidosis type IIIB (MPS IIIB) also known as Sanfilippo syndrome type B. This disorder results from mutations that impair NAGLU activity causing heparan sulfate accumulation and neurodegeneration. Additionally deficient NAGLU activity may intersect with pathologies involving heparan sulfate binding proteins impacting cellular signaling and contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the degradation of heparan sulfate.
See full target information NAGLU
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108330 PubMed38025785

2023

Targeted down-regulation of SRSF1 exerts anti-cancer activity in OSCC through impairing lysosomal function and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Qu,Ying He,Yijuan Wang,Zhengxue Han,Lizheng Qin

Journal of medicinal chemistry 66:1790-1808 PubMed36696678

2023

-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria De Pasquale,Anna Esposito,Gianluca Scerra,Melania Scarcella,Mariangela Ciampa,Antonietta Luongo,Daniele D'Alonzo,Annalisa Guaragna,Massimo D'Agostino,Luigi Michele Pavone

Aging cell 21:e13707 PubMed36087066

2022

The lysosomal proteome of senescent cells contributes to the senescence secretome.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Rovira,Rebecca Sereda,David Pladevall-Morera,Valentina Ramponi,Ines Marin,Mate Maus,Julio Madrigal-Matute,Antonio Díaz,Fernando García,Javier Muñoz,Ana María Cuervo,Manuel Serrano

Frontiers in cell and developmental biology 9:797047 PubMed35155448

2022

Downregulation of NAGLU in VEC Increases Abnormal Accumulation of Lysosomes and Represents a Predictive Biomarker in Early Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Changchang Xing,Zhongyi Jiang,Yi Wang

Experimental cell research 407:112785 PubMed34411609

2021

Disease modeling for Mucopolysaccharidosis type IIIB using patient derived induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Yu-Shan Cheng,Shu Yang,Manju Swaroop,Miao Xu,Wenwei Huang,Wei Zheng

Biochimica et biophysica acta. Molecular cell research 1868:119113 PubMed34329663

2021

Competitive binding of extracellular accumulated heparan sulfate reduces lysosomal storage defects and triggers neuronal differentiation in a model of Mucopolysaccharidosis IIIB.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria De Pasquale,Gianluca Scerra,Melania Scarcella,Massimo D'Agostino,Luigi Michele Pavone

Cell death & disease 9:40 PubMed29348482

2018

EGFR activation triggers cellular hypertrophy and lysosomal disease in NAGLU-depleted cardiomyoblasts, mimicking the hallmarks of mucopolysaccharidosis IIIB.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria De Pasquale,Antonio Pezone,Patrizia Sarogni,Alfonso Tramontano,Gabriele Giacomo Schiattarella,Vittorio Enrico Avvedimento,Simona Paladino,Luigi Michele Pavone
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB154508

Anti-NAGLU/NAG antibody

primary-antibodies

naglu-nag-antibody-ab154508

0

(0 reviews)

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