AB25968

Anti-NAIP antibody

Name: Anti-NAIP antibody (ab25968) | Abcam
Brand: Abcam Limited
SKU: AB25968
Price: 585 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(13 Publications)

Rabbit Polyclonal NAIP antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human NAIP.

View Alternative Names

BIRC1, NAIP, Baculoviral IAP repeat-containing protein 1, Neuronal apoptosis inhibitory protein

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (AB25968)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (AB25968)

Immunofluorescence of NAIP in A549 cells using ab25968 at 20 ug/ml.

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (AB25968)

ab25968 at 10μg/ml staining NAIP in A549 cells by ICC/IF

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Western blot - Anti-NAIP antibody (AB25968)

Western Blot of human tonsil lysate labeling NAIP with Anti-NAIP antibody (ab25968) at 1μg/ml.

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Western blot - Anti-NAIP antibody (ab25968)

Predicted band size: 160 kDa

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Western blot - Anti-NAIP antibody (AB25968)

Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Minor, lower molecular weight bands may represent alternately spliced forms.

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Western blot - Anti-NAIP antibody (ab25968)

Predicted band size: 160 kDa

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AbReview29526****

Western blot - Anti-NAIP antibody (AB25968)

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Western blot - Anti-NAIP antibody (ab25968) at 1/1500 dilution

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Mouse RAW 264.7 cell lysate at 20 µg

Secondary

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HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 160 kDa

Observed band size: 110 kDa,150 kDa

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Exposure time: 1min

This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ELISA, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human NAIP. The exact immunogen used to generate this antibody is proprietary information.

Q13075

Specificity

ab25968 recognises Neuronal apoptosis inhibitor protein (NAIP).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAIP also known as NLR family apoptosis inhibitory protein is a member of the nucleotide-binding oligomerization domain-like receptor (NLR) family. It plays an important role in cellular defense mechanisms. NAIP has a mass of approximately 140 kDa. It is mostly expressed in the central nervous system particularly within neurons as well as in some immune cells like macrophages. This protein functions as an inhibitor of apoptotic processes providing a protective mechanism against unwanted cell death.

Biological function summary

The primary function of NAIP is to regulate inflammation and modulate the immune response. NAIP forms part of a larger protein complex known as the inflammasome specifically the NAIP/NLRC4 inflammasome. This complex recognizes bacterial components like flagellin leading to the activation of caspase-1 which then initiates the maturation and secretion of pro-inflammatory cytokines such as interleukin-1 beta (IL-1β). By contributing to inflammasome activation NAIP is essential for the organism's immune defense against certain intracellular pathogens.

Pathways

NAIP plays a significant role in the inflammatory and immune signaling pathways. It is closely linked with the IL-1 signaling pathway and the innate immune pathway. Through these pathways NAIP interacts functionally with other proteins such as NLRC4 and caspase-1. The interaction of NAIP within these pathways permits a finely-tuned response to pathogen encounters and maintains homeostasis within immune signaling.

NAIP's malfunction or alteration associates with neurodegenerative diseases and certain cancers. For instance defects in NAIP function have been linked to spinal muscular atrophy where reduced levels of NAIP correlate with the severity of the condition. In cancer NAIP's role in inhibiting apoptosis might contribute to tumor progression by allowing abnormal cells to evade programmed cell death. The interplay between NAIP and proteins such as SMN1 in spinal muscular atrophy reflects its involvement in disease mechanics emphasizing its relevance as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis : mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.. Acts as a sensor component of the NLRC4 inflammasome that specifically recognizes and binds needle protein CprI from pathogenic bacteria C.violaceum. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria such as C.violaceum and L.pneumophila.

See full target information NAIP

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Animal reproduction 20:e20220093 PubMed37228386

2023

Changes in expression levels of Nod-like receptors in the spleen of ewes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxuan Wu,Shengya Fang,Pengfei Feng,Chunjiang Cai,Leying Zhang,Ling Yang

Vaccines 10: PubMed36560538

2022

Modulation of Nod-like Receptor Expression in the Thymus during Early Pregnancy in Ewes.

Applications

Unspecified application

Species

Unspecified reactive species

Leying Zhang,Yuanjing Li,Zhenyang Zhao,Jiabao Cai,Shuxin Zhao,Ling Yang

Animals : an open access journal from MDPI 12: PubMed36496806

2022

Early Pregnancy Modulates Expression of the Nod-like Receptor Family in Lymph Nodes of Ewes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyang Zhao,Yuanjing Li,Jianhua Cao,Hongxu Fang,Leying Zhang,Ling Yang

PLoS pathogens 17:e1009417 PubMed33861800

2021

Differential recognition of HIV-stimulated IL-1β and IL-18 secretion through NLR and NAIP signalling in monocyte-derived macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Kathy Triantafilou,Christopher J K Ward,Magdalena Czubala,Robert G Ferris,Emma Koppe,Curt Haffner,Vincent Piguet,Vipulkumar K Patel,Heather Amrine-Madsen,Louise K Modis,Seth L Masters,Martha Triantafilou

American journal of cancer research 10:4287-4307 PubMed33415000

2020

Inhibition of caspase-3-mediated GSDME-derived pyroptosis aids in noncancerous tissue protection of squamous cell carcinoma patients during cisplatin-based chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Zixian Huang,Qianyu Zhang,Yan Wang,Rui Chen,Yongqiang Wang,Zhuoshan Huang,Guangming Zhou,Haigang Li,Xi Rui,Tingting Jin,Shihao Li,Yin Zhang,Zhiquan Huang

Scientific reports 7:39981 PubMed28059125

2017

Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Abadía-Molina,Virginia Morón-Calvente,Stephen D Baird,Fahad Shamim,Francisco Martín,Alex MacKenzie

EMBO molecular medicine 8:851-62 PubMed27317434

2016

USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Engel,Martina Rudelius,Jolanta Slawska,Laura Jacobs,Behnaz Ahangarian Abhari,Bettina Altmann,Julia Kurutz,Abirami Rathakrishnan,Vanesa Fernández-Sáiz,Andrä Brunner,Bianca-Sabrina Targosz,Felicia Loewecke,Christian Johannes Gloeckner,Marius Ueffing,Simone Fulda,Michael Pfreundschuh,Lorenz Trümper,Wolfram Klapper,Ulrich Keller,Philipp J Jost,Andreas Rosenwald,Christian Peschel,Florian Bassermann

Oncotarget 5:7183-97 PubMed25216531

2014

Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Si Zhang,Wenqing Tang,Shuqiang Weng,Xijun Liu,Benqiang Rao,Jianxin Gu,She Chen,Qun Wang,Xizhong Shen,Ruyi Xue,Ling Dong

Journal of leukocyte biology 89:177-87 PubMed20844241

2010

Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors.

Applications

IHC-P

Species

Human

Terese Petterson,Johan Jendholm,Anne Månsson,Anders Bjartell,Kristian Riesbeck,Lars-Olaf Cardell

Cancer letters 292:176-85 PubMed20044205

2010

Induction of neuronal apoptosis inhibitory protein expression in response to androgen deprivation in prostate cancer.

Applications

Species

Mouse

Helen H L Chiu,Theresa M K Yong,Jun Wang,Yuwei Wang,Robert L Vessella,Takeshi Ueda,Yu-Zhuo Wang,Marianne D Sadar

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NAIP antibody

Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (AB25968)

Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (AB25968)

Western blot - Anti-NAIP antibody (AB25968)

Western blot - Anti-NAIP antibody (AB25968)

Western blot - Anti-NAIP antibody (AB25968)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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