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AB248285

Anti-NAIP antibody [EPR6169] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NAIP antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BIRC1, NAIP, Baculoviral IAP repeat-containing protein 1, Neuronal apoptosis inhibitory protein

1 Images
Western blot - Anti-NAIP antibody [EPR6169] - BSA and Azide free (AB248285)
  • WB

Supplier Data

Western blot - Anti-NAIP antibody [EPR6169] - BSA and Azide free (AB248285)

This data was developed using ab129079, the same antibody clone in a different buffer formulation. We are unsure about the nature of the extra bands.

All lanes:

Western blot - Anti-NAIP antibody [EPR6169] (<a href='/en-us/products/primary-antibodies/naip-antibody-epr6169-ab129079'>ab129079</a>) at 1/20000 dilution

Lane 1:

Human placenta lysate at 15 µg

Lane 2:

Human tonsil lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 160 kDa

Observed band size: 150 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6169

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248285 is the carrier-free version of ab129079.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAIP also known as NLR family apoptosis inhibitory protein is a member of the nucleotide-binding oligomerization domain-like receptor (NLR) family. It plays an important role in cellular defense mechanisms. NAIP has a mass of approximately 140 kDa. It is mostly expressed in the central nervous system particularly within neurons as well as in some immune cells like macrophages. This protein functions as an inhibitor of apoptotic processes providing a protective mechanism against unwanted cell death.
Biological function summary

The primary function of NAIP is to regulate inflammation and modulate the immune response. NAIP forms part of a larger protein complex known as the inflammasome specifically the NAIP/NLRC4 inflammasome. This complex recognizes bacterial components like flagellin leading to the activation of caspase-1 which then initiates the maturation and secretion of pro-inflammatory cytokines such as interleukin-1 beta (IL-1β). By contributing to inflammasome activation NAIP is essential for the organism's immune defense against certain intracellular pathogens.

Pathways

NAIP plays a significant role in the inflammatory and immune signaling pathways. It is closely linked with the IL-1 signaling pathway and the innate immune pathway. Through these pathways NAIP interacts functionally with other proteins such as NLRC4 and caspase-1. The interaction of NAIP within these pathways permits a finely-tuned response to pathogen encounters and maintains homeostasis within immune signaling.

NAIP's malfunction or alteration associates with neurodegenerative diseases and certain cancers. For instance defects in NAIP function have been linked to spinal muscular atrophy where reduced levels of NAIP correlate with the severity of the condition. In cancer NAIP's role in inhibiting apoptosis might contribute to tumor progression by allowing abnormal cells to evade programmed cell death. The interplay between NAIP and proteins such as SMN1 in spinal muscular atrophy reflects its involvement in disease mechanics emphasizing its relevance as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis : mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.. Acts as a sensor component of the NLRC4 inflammasome that specifically recognizes and binds needle protein CprI from pathogenic bacteria C.violaceum. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria such as C.violaceum and L.pneumophila.
See full target information NAIP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng
View all publications
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Product promise

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