AB237535

Anti-Naked1 antibody

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(1 Publication)

Rabbit Polyclonal Naked1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NKD1 aa 250-400.

View Alternative Names

NKD, PP7246, NKD1, Protein naked cuticle homolog 1, Naked-1, hNkd, hNkd1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Naked1 antibody (AB237535)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Naked1 antibody (AB237535)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Naked1 using ab237535 at a dilution of 1/200 in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

Immunogen

Recombinant Fragment Protein within Human NKD1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity greater than 95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Naked1 also known as Nkd1 is a protein with a mass of approximately 48 kDa. It acts as an antagonist in the Wnt signaling pathway by binding to Dishevelled an intracellular protein that transmits Wnt signals. Naked1 is expressed in various tissues including the heart brain and muscles. In addition to its role in the Wnt pathway Naked1 serves as a regulatory protein providing significant control over signal transduction that influences cell fate decisions.

Biological function summary

The function of Naked1 involves modulating the activity of the Wnt/β-catenin signaling pathway. It forms part of a negative feedback loop that prevents excessive pathway activation. Naked1 dynamically interacts with components of this pathway to inhibit or fine-tune signaling transduction ensuring that cellular processes such as proliferation differentiation and migration occur correctly. This interaction is critical in developing tissues and maintaining homeostasis in adult organisms.

Pathways

Naked1 functions within the Wnt/β-catenin pathway which is significant for cell and tissue development and homeostasis. It helps regulate this pathway by interacting with Dishevelled proteins and it also closely influences the activity of Casein kinase 1 another important component of the pathway. This regulatory role ensures a balanced signaling environment which is necessary to prevent pathological conditions associated with dysregulated Wnt signaling.

Improper regulation or expression of Naked1 associates with cancer and developmental abnormalities. Naked1 interacts with and affects proteins like β-catenin which when unregulated contributes to oncogenesis. Studies suggest that abnormalities in Naked1 expression levels may lead to tumorigenesis through unchecked cellular proliferation and migration particularly in colorectal cancer. Furthermore variations in Naked1 function may contribute to congenital disorders by disrupting the tightly controlled Wnt signaling pathway during fetal development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.

See full target information NKD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:801687 PubMed35096829

2022

Identification and Validation of Key Genes of Differential Correlations in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tingna Chen,Qiuming He,Zhenxian Xiang,Rongzhang Dou,Bin Xiong

View all publications

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Product promise

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