Rabbit Polyclonal Naked1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NKD1 aa 250-400.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NKD1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q969G9
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.
Alternative names
NKD, PP7246, NKD1, Protein naked cuticle homolog 1, Naked-1, hNkd, hNkd1
Recommended products
Rabbit Polyclonal Naked1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NKD1 aa 250-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NKD1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q969G9
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Naked1 also known as Nkd1 is a protein with a mass of approximately 48 kDa. It acts as an antagonist in the Wnt signaling pathway by binding to Dishevelled an intracellular protein that transmits Wnt signals. Naked1 is expressed in various tissues including the heart brain and muscles. In addition to its role in the Wnt pathway Naked1 serves as a regulatory protein providing significant control over signal transduction that influences cell fate decisions.
- Biological function summary
The function of Naked1 involves modulating the activity of the Wnt/β-catenin signaling pathway. It forms part of a negative feedback loop that prevents excessive pathway activation. Naked1 dynamically interacts with components of this pathway to inhibit or fine-tune signaling transduction ensuring that cellular processes such as proliferation differentiation and migration occur correctly. This interaction is critical in developing tissues and maintaining homeostasis in adult organisms.
- Pathways
Naked1 functions within the Wnt/β-catenin pathway which is significant for cell and tissue development and homeostasis. It helps regulate this pathway by interacting with Dishevelled proteins and it also closely influences the activity of Casein kinase 1 another important component of the pathway. This regulatory role ensures a balanced signaling environment which is necessary to prevent pathological conditions associated with dysregulated Wnt signaling.
- Associated diseases and disorders
Improper regulation or expression of Naked1 associates with cancer and developmental abnormalities. Naked1 interacts with and affects proteins like β-catenin which when unregulated contributes to oncogenesis. Studies suggest that abnormalities in Naked1 expression levels may lead to tumorigenesis through unchecked cellular proliferation and migration particularly in colorectal cancer. Furthermore variations in Naked1 function may contribute to congenital disorders by disrupting the tightly controlled Wnt signaling pathway during fetal development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-Naked1 antibody (ab237535)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Naked1 using ab237535 at a dilution of 1/200 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
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