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AB105409

Anti-NALP12/NLRP12 antibody

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(4 Publications)

Rabbit Polyclonal NALP12/NLRP12 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NLRP12.

View Alternative Names

NALP12, PYPAF7, RNO, NLRP12, Monarch-1, PYRIN-containing APAF1-like protein 7, Regulated by nitric oxide

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody (AB105409)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody (AB105409)

Immunohistochemistry of Human brain tissue with ab105409 5 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody (AB105409)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody (AB105409)

Immunofluorescence of NALP12/NLRP12 in Human brain tissue with ab105409 at 20 ug/mL.

Western blot - Anti-NALP12/NLRP12 antibody (AB105409)
  • WB

Unknown

Western blot - Anti-NALP12/NLRP12 antibody (AB105409)

All lanes:

Western blot - Anti-NALP12/NLRP12 antibody (ab105409) at 1 µg/mL

All lanes:

Human brain tissue lysate at 15 µg

Predicted band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human NLRP12. The exact immunogen used to generate this antibody is proprietary information.

P59046

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NALP12 also known as NLRP12 is a member of the NLR (NOD-like receptor) family. This protein serves as an intracellular sensor important for recognizing pathogenic factors. NLRP12 has a mass of approximately 116 kDa. It is expressed predominantly in leukocytes particularly in monocytes and granulocytes. This expression pattern suggests an essential role in regulating immune responses.
Biological function summary

NLRP12 functions as part of the inflammasome complex. It contributes to the modulation of inflammatory cytokines like IL-1β. The protein plays a critical role in the innate immune system by controlling inflammatory signaling pathways. By down-regulating the NF-kB and MAPK pathways NLRP12 helps prevent excessive inflammatory responses maintaining homeostasis within the immune system.

Pathways

NLRP12 is integrally involved in the NF-kB and MAPK signaling pathways. These pathways are essential for inflammatory response and immune regulation. Related proteins include NLRP3 and IKK which also participate in these pathways. NLRP12 negatively regulates NF-kB activation assisting to keep inflammation in check. Understanding these interactions provides insights into how the immune system balances response and regulation.

NLRP12 mutations have associations with autoinflammatory disorders such as familial cold autoinflammatory syndrome and lymphoid malignancies. These conditions arise due to dysregulated inflammatory responses. Through its connection with the NF-kB pathway NLRP12 interacts with the IKK complex. Misregulation here can lead to an increased predisposition to these inflammatory disorders highlighting the importance of NLRP12 in immune homeostasis and pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role as an potent mitigator of inflammation (PubMed : 30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed : 15489334, PubMed : 17947705). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed : 30559449). In turn, promotes bacterial tolerance (PubMed : 30559449). Also inhibits the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation (PubMed : 30902577). Also acts as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (By similarity).
See full target information NLRP12
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405834 PubMed39527457

2024

AIP1 Regulates Ocular Angiogenesis Via NLRP12-ASC-Caspase-8 Inflammasome-Mediated Endothelial Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghao Li,Yimeng Sun,Dasen Xie,Hui Chen,Qi Zhang,Shaochong Zhang,Feng Wen,Jing-Song Ou,Min Zhang,Lishi Su,Xuri Li,Wei-Ping Wen,Wei Chi

Cell biochemistry and biophysics 81:727-735 PubMed37658975

2023

Inhibited Expression of NLRP12 Promotes the Development of Triple-Negative Breast Cancer by Activating the NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbin Kuang,Qingdan Gu,Ying Zhou,Xiaoqin Xiao,Dabao He,Qiuchan Deng

Frontiers in immunology 12:744880 PubMed34956178

2021

A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus Response.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Li,Zhuo Dong,Yan Liu,Weifeng Song,Jieying Pu,Guanmin Jiang,Yongjian Wu,Lei Liu,Xi Huang

Experimental and therapeutic medicine 18:1486-1492 PubMed31363381

2019

Peripheral blood miR-372 as a biomarker for ulcerative colitis via direct targeting of NLRP12.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdie Shen,Li'Na Meng
View all publications
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Product promise

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