Rabbit Polyclonal NALP12/NLRP12 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NLRP12.
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Publications
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- Cell biochemistry and biophysics 81:727-7352023Inhibited Expression of NLRP12 Promotes the Development of Triple-Negative Breast Cancer by Activating the NF-κB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenbin Kuang,Qingdan Gu,Ying Zhou,Xiaoqin Xiao,Dabao He,Qiuchan DengPubMed 37658975
- Frontiers in immunology 12:7448802021A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingyu Li,Zhuo Dong,Yan Liu,Weifeng Song,Jieying Pu,Guanmin Jiang,Yongjian Wu,Lei Liu,Xi HuangPubMed 34956178
- Experimental and therapeutic medicine 18:1486-14922019Peripheral blood miR-372 as a biomarker for ulcerative colitis via direct targeting of NLRP12.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengdie Shen et. al.PubMed 31363381
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NLRP12. The exact immunogen used to generate this antibody is proprietary information. Database link P59046
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Plays an essential role as an potent mitigator of inflammation (PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:15489334, PubMed:17947705). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation (PubMed:30902577). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (By similarity).
Alternative names
NALP12, PYPAF7, RNO, NLRP12, Monarch-1, PYRIN-containing APAF1-like protein 7, Regulated by nitric oxide
Recommended products
Rabbit Polyclonal NALP12/NLRP12 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NLRP12.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NLRP12. The exact immunogen used to generate this antibody is proprietary information. Database link P59046
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NALP12 also known as NLRP12 is a member of the NLR (NOD-like receptor) family. This protein serves as an intracellular sensor important for recognizing pathogenic factors. NLRP12 has a mass of approximately 116 kDa. It is expressed predominantly in leukocytes particularly in monocytes and granulocytes. This expression pattern suggests an essential role in regulating immune responses.
- Biological function summary
NLRP12 functions as part of the inflammasome complex. It contributes to the modulation of inflammatory cytokines like IL-1β. The protein plays a critical role in the innate immune system by controlling inflammatory signaling pathways. By down-regulating the NF-kB and MAPK pathways NLRP12 helps prevent excessive inflammatory responses maintaining homeostasis within the immune system.
- Pathways
NLRP12 is integrally involved in the NF-kB and MAPK signaling pathways. These pathways are essential for inflammatory response and immune regulation. Related proteins include NLRP3 and IKK which also participate in these pathways. NLRP12 negatively regulates NF-kB activation assisting to keep inflammation in check. Understanding these interactions provides insights into how the immune system balances response and regulation.
- Associated diseases and disorders
NLRP12 mutations have associations with autoinflammatory disorders such as familial cold autoinflammatory syndrome and lymphoid malignancies. These conditions arise due to dysregulated inflammatory responses. Through its connection with the NF-kB pathway NLRP12 interacts with the IKK complex. Misregulation here can lead to an increased predisposition to these inflammatory disorders highlighting the importance of NLRP12 in immune homeostasis and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody (ab105409)
Immunofluorescence of NALP12/NLRP12 in Human brain tissue with ab105409 at 20 ug/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody (ab105409)
Immunohistochemistry of Human brain tissue with ab105409 5 μg/mL.
Western blot - Anti-NALP12/NLRP12 antibody (ab105409)
All lanes: Western blot - Anti-NALP12/NLRP12 antibody (ab105409) at 1 µg/mL
All lanes: Human brain tissue lysate at 15 µg
Predicted band size: 120 kDa
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