Rabbit Recombinant Monoclonal NALP12/NLRP12 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409817.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409816.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409815.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409814.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (AB308250)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | IP | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Human, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
Target data
Function
Plays an essential role as an potent mitigator of inflammation (PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:15489334, PubMed:17947705). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation (PubMed:30902577). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (By similarity).
Alternative names
NALP12, PYPAF7, RNO, NLRP12, Monarch-1, PYRIN-containing APAF1-like protein 7, Regulated by nitric oxide
Recommended products
Rabbit Recombinant Monoclonal NALP12/NLRP12 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26985-39
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NALP12 also known as NLRP12 is a member of the NLR (NOD-like receptor) family. This protein serves as an intracellular sensor important for recognizing pathogenic factors. NLRP12 has a mass of approximately 116 kDa. It is expressed predominantly in leukocytes particularly in monocytes and granulocytes. This expression pattern suggests an essential role in regulating immune responses.
- Biological function summary
NLRP12 functions as part of the inflammasome complex. It contributes to the modulation of inflammatory cytokines like IL-1β. The protein plays a critical role in the innate immune system by controlling inflammatory signaling pathways. By down-regulating the NF-kB and MAPK pathways NLRP12 helps prevent excessive inflammatory responses maintaining homeostasis within the immune system.
- Pathways
NLRP12 is integrally involved in the NF-kB and MAPK signaling pathways. These pathways are essential for inflammatory response and immune regulation. Related proteins include NLRP3 and IKK which also participate in these pathways. NLRP12 negatively regulates NF-kB activation assisting to keep inflammation in check. Understanding these interactions provides insights into how the immune system balances response and regulation.
- Associated diseases and disorders
NLRP12 mutations have associations with autoinflammatory disorders such as familial cold autoinflammatory syndrome and lymphoid malignancies. These conditions arise due to dysregulated inflammatory responses. Through its connection with the NF-kB pathway NLRP12 interacts with the IKK complex. Misregulation here can lead to an increased predisposition to these inflammatory disorders highlighting the importance of NLRP12 in immune homeostasis and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409817.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling NALP12/NLRP12 with ab308250 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human testis. The section was incubated with ab308250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409816.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NALP12/NLRP12 with ab308250 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen (PMID: 12759408). The section was incubated with ab308250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409815.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling NALP12/NLRP12 with ab308250 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in human colon. The section was incubated with ab308250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250), expandable thumbnail](https://content.abcam.com/products/images/nalp12-nlrp12-antibody-epr26985-39-ab308250--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409814.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP12/NLRP12 antibody [EPR26985-39] (ab308250)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labelling NALP12/NLRP12 with ab308250 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on HEK293T cells co-transfected with a human NLRP12 expression vector containing a Myc-his tag, no staining on HEK-293T cells transfected with empty vector containing a Myc-his tag. The section was incubated with ab308250 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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