AB204111

Anti-NALP4 antibody

Name: Anti-NALP4 antibody (ab204111) | Abcam
Brand: Abcam Limited
SKU: AB204111

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(1 Publication)

Rabbit Polyclonal NALP4 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NLRP4 aa 350-450 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NALP4, PAN2, PYPAF4, RNH2, NLRP4, Cancer/testis antigen 58, PAAD and NACHT-containing protein 2, PYRIN and NACHT-containing protein 2, PYRIN-containing APAF1-like protein 4, Ribonuclease inhibitor 2, CT58

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP4 antibody (AB204111)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP4 antibody (AB204111)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling NALP4 with ab204111 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP4 antibody (AB204111)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling NALP4 with ab204111 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP4 antibody (AB204111)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded rat colitis tissue labeling NALP4 with ab204111 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NLRP4 aa 350-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96MN2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50-1/200.</p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50-1/200.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NALP4 also known as NLRP4 is a member of the NOD-like receptor (NLR) family. This protein has a mass of approximately 116 kDa. NALP4 is present mainly in immune cells such as macrophages and dendritic cells. It is located within the cytoplasm where it participates in the recognition of specific cellular ligands and forms a multi-protein complex known as the inflammasome.

Biological function summary

NLRP4 plays a role in innate immune response regulation. Part of the inflammasome complex it senses intracellular danger signals and plays a role in inflammatory processes. NLRP4 involves the recruitment of other components such as ASC (apoptosis-associated speck-like protein) and pro-caspase-1 leading to the activation of caspase-1 and the maturation of pro-inflammatory cytokines like IL-1β and IL-18.

Pathways

NLRP4 contributes to the regulation of the inflammatory signaling cascade a subset of the broader immune response pathway. NLRP4 connects with proteins such as NLRP3 and NLRC4 within these pathways highlighting its role in modulating cytokine release and inflammatory responses. By integrating signals NLRP4 influences both protective and potentially harmful inflammation maintaining tissue homeostasis.

NLRP4 has associations with autoimmune conditions and chronic inflammatory diseases. It is linked to diseases such as systemic lupus erythematosus and Crohn’s disease where dysregulation of NLRP4 activities contributes to pathogenesis. In these cases interactions between NLRP4 and proteins like IL-1β or ASC may exacerbate inflammatory processes offering potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

May be involved in inflammation and recognition of cytosolic pathogen-associated molecular patterns (PAMPs) not intercepted by membrane-bound receptors. Acts as a negative regulator of the type I interferon signaling pathway by serving as an adapter to promote DTX4-mediated ubiquitination of activated TBK1, and its subsequent degradation. Suppresses NF-kappaB induction by the cytokines TNFA and IL1B, suggesting that it operates at a point of convergence in these two cytokine signaling pathways.

See full target information NLRP4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 40:140-151 PubMed39415627

2024

H3K27 Acetylation-Activated GLDC Accelerated the Advancement of Oral Squamous Cell Carcinoma by Suppressing the p53 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Xu,Qingfeng Xu,Haibing Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NALP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP4 antibody (AB204111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP4 antibody (AB204111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NALP4 antibody (AB204111)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise