AB215180

Anti-NALP5 antibody - C-terminal

Name: Anti-NALP5 antibody - C-terminal (ab215180) | Abcam
Brand: Abcam Limited
SKU: AB215180
Price: 510 USD
Availability: InStock

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(1 Publication)

Goat Polyclonal NALP5 antibody. C-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NLRP5 aa 1150 to C-terminus.

View Alternative Names

MATER, NALP5, NLRP5, Mater protein homolog, Maternal Antigen that Embryos Require

1 Images

Supplier Data

Western blot - Anti-NALP5 antibody - C-terminal (AB215180)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-NALP5 antibody - C-terminal (ab215180) at 2 µg/mL

All lanes:

Human ovary lysate in RIPA buffer at 35 µg

Predicted band size: 134 kDa

Observed band size: ~130 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human NLRP5 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P59047

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab215180 was purified from goat serum by ammonium sulphate precipitation followed by immunogen affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NALP5 also known as NLRP5 or Mater is a member of the NOD-like receptor (NLR) family and has significant mechanical functions in cellular processes. The protein weighs approximately 136 kDa and is mainly expressed in oocytes and early embryos in mammals. Its primary role involves regulation of the innate immune response by detecting intracellular threats and forming inflammasomes. NALP5 contains several important domains including the pyrin domain (PYD) NACHT and leucine-rich repeat domain which contribute to its function in cellular defense mechanisms.

Biological function summary

NALP5 plays critical roles in early embryonic development and successful reproduction. It acts as part of the multiprotein complex known as the inflammasome which is essential for the activation of inflammatory cytokines such as IL-1β. This protein is important for oocyte maturation and early embryogenesis impacting fertility and successful pregnancy outcomes. In the developmental context alterations in the function of NALP5 can affect embryonic cell survival and overall developmental competence.

Pathways

NALP5 is integral in facilitating inflammasome assembly and subsequent activation of inflammatory pathways. It significantly influences the immune response modulation through pathways like the NF-kB signaling pathway. Furthermore NALP5 interacts with proteins such as ASC (apoptosis-associated speck-like protein) to mediate cytokine maturation and inflammation. This protein also connects with caspase-1 a critical component necessary for inflammasome function and cytokine activation.

NALP5 relates directly to autoimmune diseases and reproductive disorders. Altered expression or mutations in NALP5 can result in female infertility and have been studied in the context of autoimmune abnormalities. The protein connects to various disease-associated proteins such as NALP3 and other inflammasome components influencing disease progress in conditions marked by inflammation dysregulation. Understanding the role of NALP5 can therefore provide insights into the pathology of related disorders and open avenues for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development. The SCMC complex is a structural constituent of cytoplasmic lattices, which consist in fibrous structures found in the cytoplasm of oocytes and preimplantation embryos. They are required to store maternal proteins critical for embryonic development, such as proteins that control epigenetic reprogramming of the preimplantation embryo, and prevent their degradation or activation. Required for the localization of cortical granules to the cortex of oocytes, via association with the cortical actin scaffold. Required for cortical actin clearance prior to oocyte exocytosis and prevention of polyspermy. Involved in regulating post-fertilization Ca(2+) release and endoplasmic reticulum storage (ER) storage via regulation of cellular localization. May be involved in the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early stage embryos, independent of its role in CPL formation.

See full target information NLRP5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:435 PubMed38902235

2024

ALKBH4 impedes 5-FU Sensitivity through suppressing GSDME induced pyroptosis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jiang,Zhiman Zhu,Lina Ding,Wenqi Du,Dongsheng Pei

View all publications

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Product promise

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Anti-NALP5 antibody - C-terminal

Western blot - Anti-NALP5 antibody - C-terminal (AB215180)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise