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AB173368

Anti-Nanog antibody [23D2-3C6]

5

(2 Reviews)

|

(12 Publications)

Mouse Monoclonal NANOG antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NANOG.

View Alternative Names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (AB173368)

Immunofluorescent analysis of HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (AB173368)

Flow cytometric analysis of H9 embryonic stem cells labeling Nanog with ab173368 at 1/100 (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (AB173368)

Immunofluorescent analysis of NTERA-2 and HeLa cells (formalin-fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/50 dilution (green) followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (AB173368)

Immunofluorescent analysis of H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (AB173368)

Flow cytometric analysis of HEL 11.4 induced IPS cells labeling Nanog with ab173368 at 1/00 dilution (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary antibody at 1/200 dilution.

Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • WB

Supplier Data

Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368)

4-20% Tris-HCl polyacrylamide gel.

All lanes:

Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1:

NCCIT nuclear fraction lysate at 60 µg

Lane 2:

NCCIT cytoplasmic fraction lysate at 60 µg

Secondary

All lanes:

goat anti-mouse IgG-HRP at 1/20000 dilution

Predicted band size: 34 kDa

true

Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • WB

Supplier Data

Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368)

4-20% Tris-HCl polyacrylamide gel

All lanes:

Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1:

NCCIT lysate at 60 µg

Lane 2:

NTERA-2 lysate at 60 µg

Lane 3:

HeLa lysate at 60 µg

Secondary

All lanes:

goat anti-mouse IgG-HRP at 1/20000 dilution

Predicted band size: 34 kDa

true

Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368)
  • WB

Unknown

Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368)

All lanes:

Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1:

NTERA-2 cell line at 30 µg

Lane 2:

NCCIT cell line at 30 µg

Lane 3:

iPSC cell line at 30 µg

Lane 4:

iPSC differentiated to definitive endoderm at 30 µg

Lane 5:

HeLa cell line at 30 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/40000 dilution

Predicted band size: 34 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

23D2-3C6

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NANOG.

Q9H9S0

Specificity

ab173368 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. It shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed : 25825768). Able to autorepress its expression in differentiating (ES) cells : binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
See full target information NANOG
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2924:93-100 PubMed40307637

2025

A Robust Protocol for Pluripotent Stem Cell Modeling of 3D Chamber-Like Cardiac Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Michel Pucéat

Cell & bioscience 14:65 PubMed38778363

2024

Employment of diverse in vitro systems for analyzing multiple aspects of disease, hereditary hemorrhagic telangiectasia (HHT).

Applications

Unspecified application

Species

Unspecified reactive species

Hyebin Koh,Woojoo Kang,Ying-Ying Mao,Jisoo Park,Sangjune Kim,Seok-Ho Hong,Jong-Hee Lee

Cancers 16: PubMed38398205

2024

Transduction Efficiency of Zika Virus E Protein Pseudotyped HIV-1 and Its Oncolytic Activity Tested in Primary Glioblastoma Cell Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Patrick Formanski,Hai Dang Ngo,Vivien Grunwald,Celine Pöhlking,Jana Sue Jonas,Dominik Wohlers,Birco Schwalbe,Michael Schreiber

International journal of molecular sciences 24: PubMed37833934

2023

Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vivien Grunwald,Hai Dang Ngo,Jan Patrick Formanski,Jana Sue Jonas,Celine Pöhlking,Birco Schwalbe,Michael Schreiber

International journal of molecular sciences 24: PubMed37108645

2023

Vascular Stem Cells and the Role of B-Raf Kinase in Survival, Proliferation, and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dipali Goyal,Sean W Limesand,Ravi Goyal

Advanced materials (Deerfield Beach, Fla.) 35:e2205082 PubMed36796025

2023

Expanding Embedded 3D Bioprinting Capability for Engineering Complex Organs with Freeform Vascular Networks.

Applications

Unspecified application

Species

Unspecified reactive species

Yongcong Fang,Yihan Guo,Bingyan Wu,Zibo Liu,Min Ye,Yuanyuan Xu,Mengke Ji,Li Chen,Bingchuan Lu,Kaiji Nie,Zixuan Wang,Jianbin Luo,Ting Zhang,Wei Sun,Zhuo Xiong

Science advances 8:eabq6720 PubMed36525500

2022

Modeling long QT syndrome type 2 on-a-chip via in-depth assessment of isogenic gene-edited 3D cardiac tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Jaimeson Veldhuizen,Helen F Mann,Nina Karamanova,Wade D Van Horn,Raymond Q Migrino,David Brafman,Mehdi Nikkhah

Developmental biology 494:60-70 PubMed36509125

2022

Synchronisation of apical constriction and cell cycle progression is a conserved behaviour of pseudostratified neuroepithelia informed by their tissue geometry.

Applications

Unspecified application

Species

Unspecified reactive species

Ioakeim Ampartzidis,Christoforos Efstathiou,Francesco Paonessa,Elliott M Thompson,Tyler Wilson,Conor J McCann,Nicholas DE Greene,Andrew J Copp,Frederick J Livesey,Nicola Elvassore,Giovanni G Giobbe,Paolo De Coppi,Eirini Maniou,Gabriel L Galea

NPJ breast cancer 8:115 PubMed36309503

2022

LINC00589-dominated ceRNA networks regulate multiple chemoresistance and cancer stem cell-like properties in HER2 breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wendong Bai,Hongyan Peng,Jiarui Zhang,Yongmei Zhao,Zhijun Li,Xuelian Feng,Jiang Zhang,Fei Liang,Li Wang,Nan Zhang,Yize Li,Huayu Zhu,Qiuhe Ji

Molecular medicine reports 24: PubMed34036375

2021

lncRNA TUG1 inhibits the cancer stem cell‑like properties of temozolomide‑resistant glioma cells by interacting with EZH2.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiang Cao,Wenying Chai,Yonggang Wang,Dang Tang,Dongchuan Shao,Hai Song,Jiang Long
View all publications
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