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Mouse Monoclonal NANOG antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NANOG.


Images

Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368), expandable thumbnail
  • Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (AB173368), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (AB173368), expandable thumbnail
  • Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (AB173368), expandable thumbnail
  • Western blot - Anti-Nanog antibody [23D2-3C6] (AB173368), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human NANOG. Database link Q9H9S0

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Chimpanzee
Predicted
Predicted
Predicted
Cynomolgus monkey
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Chimpanzee, Cynomolgus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Predicted
Predicted

Species
Chimpanzee, Cynomolgus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50.00000 - 1/100.00000
Notes

-

Predicted
Predicted

Species
Chimpanzee, Cynomolgus monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Alternative names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

Recommended products

Mouse Monoclonal NANOG antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NANOG.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human NANOG. Database link Q9H9S0
Clone number
23D2-3C6
Purification technique
Affinity purification Protein A
Specificity

ab173368 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. It shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.

Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Associated diseases and disorders

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.

Product promise

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8 product images

  • Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

    All lanes: Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

    Lane 1: NTERA-2 cell line at 30 µg

    Lane 2: NCCIT cell line at 30 µg

    Lane 3: iPSC cell line at 30 µg

    Lane 4: iPSC differentiated to definitive endoderm at 30 µg

    Lane 5: HeLa cell line at 30 µg

    Secondary

    All lanes: Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/40000 dilution

    Predicted band size: 34 kDa

  • Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Flow cytometric analysis of H9 embryonic stem cells labeling Nanog with ab173368 at 1/100 (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary at 1/200 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Immunofluorescent analysis of H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

  • Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Flow cytometric analysis of HEL 11.4 induced IPS cells labeling Nanog with ab173368 at 1/00 dilution (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary antibody at 1/200 dilution.

  • Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

    4-20% Tris-HCl polyacrylamide gel.

    All lanes: Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

    Lane 1: NCCIT nuclear fraction lysate at 60 µg

    Lane 2: NCCIT cytoplasmic fraction lysate at 60 µg

    Secondary

    All lanes: goat anti-mouse IgG-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa

  • Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

    4-20% Tris-HCl polyacrylamide gel

    All lanes: Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

    Lane 1: NCCIT lysate at 60 µg

    Lane 2: NTERA-2 lysate at 60 µg

    Lane 3: HeLa lysate at 60 µg

    Secondary

    All lanes: goat anti-mouse IgG-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Immunofluorescent analysis of HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

    Immunofluorescent analysis of NTERA-2 and HeLa cells (formalin-fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/50 dilution (green) followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

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