Rabbit Polyclonal NANOG antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human NANOG.
Images
Publications
Filter by
- Cell death & disease 13:9762022BNIP3-dependent mitophagy safeguards ESC genomic integrity via preventing oxidative stress-induced DNA damage and protecting homologous recombination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Zhao,Kun Liu,Lin Zhang,Zheng Li,Liang Wang,Jiani Cao,Youqing Xu,Aihua Zheng,Quan Chen,Tongbiao ZhaoPubMed 36402748
- Clinical and translational medicine 10:e1392020Transfer of metastatic traits via miR-200c in extracellular vesicles derived from colorectal cancer stem cells is inhibited by atractylenolide I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongxin Tang,Xiaofen Xu,Jialiang Ying,Tian Xie,Gang CaoPubMed 32898324
- International journal of oncology 57:743-7552020Activation of PGK1 under hypoxic conditions promotes glycolysis and increases stem cell‑like properties and the epithelial‑mesenchymal transition in oral squamous cell carcinoma cells via the AKT signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYadong Zhang et. al.PubMed 32705252
- American journal of translational research 12:3078-30882020Senolytic controls bone marrow mesenchymal stem cells fate improving bone formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDianying Zhang et. al.PubMed 32655832
- BMC molecular and cell biology 21:412020Substance P enhances the local activation of NKR-expressing c-kit cardiac progenitor cells in right atrium of ischemia/reperfusion-injured heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Mi Jeong et. al.PubMed 32517655
- Zygote (Cambridge, England) 28:175-1822020Establishment of a feeder and serum-free culture system for human embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiYun Wang et. al.PubMed 31965957
- International journal of clinical and experimental pathology 12:4361-43792019miR-601 inhibits proliferation, migration and invasion of prostate cancer stem cells by targeting KRT5 to inactivate the Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongbing Du,Xinghuan Wang,Rui Dong,Dongliang Hu,Yaoyi XiongPubMed 31933840
- Experimental & molecular medicine 51:1-202019Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Cheng Guo et. al.PubMed 31757938
- Biomaterials 222:1194562019Chiral geometry regulates stem cell fate and activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingqing Dong et. al.PubMed 31476662
- Cell cycle (Georgetown, Tex.) 18:2414-24312019GREM2 maintains stem cell-like phenotypes in gastric cancer cells by regulating the JNK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAo Ran et. al.PubMed 31345097
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NANOG. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9S0
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Species Mouse | Dilution info 2 µg/mL | Notes - |
Species Rat | Dilution info 2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 2 µg/mL | Notes - |
Species Rat | Dilution info 2 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Alternative names
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Recommended products
Rabbit Polyclonal NANOG antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human NANOG.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NANOG. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9S0
- Purification technique
- Affinity purification Immunogen
- Specificity
At least two isoforms of Nanog are known to exist; ab106465 will
detect both.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
- Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
- Pathways
Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
- Associated diseases and disorders
Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab106465)
Immunofluorescence of NANOG in Human Spleen cells using ab106465 at 20 ug/ml.
Western blot - Anti-Nanog antibody (ab106465)
Lane 1: Western blot - Anti-Nanog antibody (ab106465) at 1 µg/mL
Lane 2: Western blot - Anti-Nanog antibody (ab106465) at 2 µg/mL
All lanes: Human spleen tissue lysate at 15 µg
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab106465)
ab106465, at 5 µg/ml, staining Nanog in Human spleen tissue by Immunohistochemistry.
Western blot - Anti-Nanog antibody (ab106465)
1 h incubation at RT in 8% NFDM/TBST.
All lanes: Western blot - Anti-Nanog antibody (ab106465) at 2 µg/mL
All lanes: HeLa cell lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugat at 1/10000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab106465)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Nanog with ab106465 at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-Nanog antibody (ab106465)
1 h incubation at RT in 8% NFDM/TBST.
All lanes: Western blot - Anti-Nanog antibody (ab106465) at 2 µg/mL
Lane 1: Mouse testis tissue lysate at 15 µg
Lane 2: Rat testis tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugat at 1/10000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab106465)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Nanog with ab106465 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab106465)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Nanog with ab106465 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com