Anti-Nanog antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB106465)

Immunofluorescence of NANOG in Human Spleen cells using ab106465 at 20 ug/ml.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (AB106465)

ab106465, at 5 µg/ml, staining Nanog in Human spleen tissue by Immunohistochemistry.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (AB106465)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Nanog with ab106465 at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (AB106465)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Nanog with ab106465 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (AB106465)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Nanog with ab106465 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-Nanog antibody (AB106465)

1 h incubation at RT in 8% NFDM/TBST.

All lanes:

Western blot - Anti-Nanog antibody (ab106465) at 2 µg/mL

All lanes:

HeLa cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugat at 1/10000 dilution

false

• WB

Unknown

Western blot - Anti-Nanog antibody (AB106465)

Lane 1:

Western blot - Anti-Nanog antibody (ab106465) at 1 µg/mL

Lane 2:

Western blot - Anti-Nanog antibody (ab106465) at 2 µg/mL

All lanes:

Human spleen tissue lysate at 15 µg

Predicted band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-Nanog antibody (AB106465)

1 h incubation at RT in 8% NFDM/TBST.

All lanes:

Western blot - Anti-Nanog antibody (ab106465) at 2 µg/mL

Lane 1:

Mouse testis tissue lysate at 15 µg

Lane 2:

Rat testis tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugat at 1/10000 dilution

false