Anti-Nanog antibody
5
(16 Reviews)
|
(342 Publications)
Anti-Nanog antibody (ab21624) is a rabbit polyclonal antibody detecting Nanog in Western Blot, ICC/IF, ELISA. Suitable for Human,.
- Over 280 publications
- Trusted since 2005
View Alternative Names
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)
ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
- ICC/IF
AbReview53716****
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (AB21624)
Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.
Image courtesy of Mick Wauchope
- WB
Unknown
Western blot - Anti-Nanog antibody (AB21624)
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.
All lanes:
Western blot - Anti-Nanog antibody (ab21624) at 1/1000 dilution
Lane 1:
whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2:
whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size: 34 kDa
Observed band size: 38 kDa
false
- WB
Supplier Data
Western blot - Anti-Nanog antibody (AB21624)
All lanes:
Western blot - Anti-Nanog antibody (ab21624) at 1/200 dilution
Lane 1:
Human fetal fibroblast lysate
Lane 2:
Human iPS cell lysate
Predicted band size: 34 kDa
false
- sELISA
Unknown
Sandwich ELISA - Anti-Nanog antibody (AB21624)
Standard Curve for Nanog (Analyte : Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
Reactivity data
Product details
Anti-Nanog antibody (ab21624) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, samples.
What is the molecular weight of Nanog?
Anti-Nanog (ab21624) specifically detects a band for Nanog (UniProt: Q9H9S0) at a molecular weight of 34kDa.
Trusted by the scientific community
Anti-Nanog (ab21624) was first used in a scientific publication in 2005 and has been cited over 280 times in peer-reviewed journals.
Reviewed by scientists
Anti-Nanog (ab21624) has over 15 independent reviews from customers.
Properties and storage information
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Purification technique
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Shipped at conditions
Appropriate short-term storage duration
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Appropriate long-term storage conditions
Aliquoting information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
Pathways
Nanog is deeply embedded in the Wnt/Β-Catenin and TGF-Β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/Β-Catenin pathway Nanog works alongside proteins like Β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-Β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
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Target data
Publications (342)
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70958 PubMed40873389
2025
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Scientific reports 15:29484 PubMed40796594
2025
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iScience 28:112843 PubMed40662194
2025
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Biomolecules 15: PubMed40149974
2025
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Scientific reports 15:7095 PubMed40016322
2025
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Nature communications 16:1982 PubMed40011434
2025
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The Journal of biological chemistry 301:108235 PubMed39880095
2025
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Journal of molecular and cellular cardiology plus 10: PubMed39742339
2025
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PloS one 19:e0311761 PubMed39388496
2024
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Stem cell research 81:103548 PubMed39353357
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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