Anti-Nanog antibody ab21624 is a rabbit polyclonal antibody that is used in Nanog western blotting and immunofluorescence. Suitable for human samples.
- Tried and trusted by researchers since 2005
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- Biology direct 18:802023RCC2 promotes prostate cancer cell proliferation and migration through Hh/GLI1 signaling pathway and cancer stem-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShenghan Wang,Zhentao Lei,Wei Liu,Jie Xiong,Yuqiang Shi,Lin Yang,Qiang Gao,Kai Le,Bao ZhangPubMed 38008751
- Metabolites 13:2023Perturbations of Glutathione and Sphingosine Metabolites in Port Wine Birthmark Patient-Derived Induced Pluripotent Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVi Nguyen,Jacob Kravitz,Chao Gao,Marcelo L Hochman,Dehao Meng,Dongbao Chen,Yunguan Wang,Anil G Jegga,J Stuart Nelson,Wenbin TanPubMed 37755263
- Nature 620:863-8722023Transient naive reprogramming corrects hiPS cells functionally and epigenetically.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSam Buckberry,Xiaodong Liu,Daniel Poppe,Jia Ping Tan,Guizhi Sun,Joseph Chen,Trung Viet Nguyen,Alex de Mendoza,Jahnvi Pflueger,Thomas Frazer,Dulce B Vargas-Landín,Jacob M Paynter,Nathan Smits,Ning Liu,John F Ouyang,Fernando J Rossello,Hun S Chy,Owen J L Rackham,Andrew L Laslett,James Breen,Geoffrey J Faulkner,Christian M Nefzger,Jose M Polo,Ryan ListerPubMed 37587336
- International journal of molecular sciences 24:2023A Novel Genetic Variant in Associated with Severe Epilepsy and Intellectual Disability: Potential Implications on Neural Primary Cilia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Martins,Ana Rafaela Oliveira,Solange Martins,José Pedro Vieira,Pedro Perdigão,Ana Rita Fernandes,Luís Pereira de Almeida,Paulo Jorge Palma,Diana Bela Sequeira,João Miguel Marques Santos,Frederico Duque,Guiomar Oliveira,Ana Luísa Cardoso,João Peça,Catarina Morais SeabraPubMed 37628781
- EMBO reports 24:e564392023Reducing oxidative protein folding alleviates senescence by minimizing ER-to-nucleus H O release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Cheng,Qianzhao Ji,Lu Wang,Chih-Chen Wang,Guang-Hui Liu,Lei WangPubMed 37306027
- eLife 12:2023Allele-specific gene-editing approach for vision loss restoration in -associated retinitis pigmentosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaozhen Liu,Jing Qiao,Ruixuan Jia,Fan Zhang,Xiang Meng,Yang Li,Liping YangPubMed 37272616
- Bioactive materials 28:206-2262023Dual human iPSC-derived cardiac lineage cell-seeding extracellular matrix patches promote regeneration and long-term repair of infarcted hearts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Jiang,Ling-Ling Zhang,Fan Zhang,Wei Bi,Peng Zhang,Xiu-Jian Yu,Sen-Le Rao,Shi-Hui Wang,Qiang Li,Chen Ding,Yin Jin,Zhong-Min Liu,Huang-Tian YangPubMed 37274446
- Environmental toxicology 38:2002-20102023TRIM29 facilitates proliferation and malignancy of cholangiocarcinoma cells by activating MAPK and β-catenin pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Yang,Wenqing Bao,Haiyan Diao,Shaofei Yang,Lin Qiu,Chunjing Xu,Bin ZhaoPubMed 37219039
- Stem cell research 69:1030782023Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasuma Akter,Haochen Cui,Md Abir Hosain,Baojin DingPubMed 36965406
- Protein & cell 14:202-21620234E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifang He,Qianzhao Ji,Zeming Wu,Yusheng Cai,Jian Yin,Yiyuan Zhang,Sheng Zhang,Xiaoqian Liu,Weiqi Zhang,Guang-Hui Liu,Si Wang,Moshi Song,Jing QuPubMed 36929036
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NANOG aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9S0
Reactivity data
Select an application| WB | sELISA | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 0.5 µg/mL | Notes For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Alternative names
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Recommended products
Anti-Nanog antibody ab21624 is a rabbit polyclonal antibody that is used in Nanog western blotting and immunofluorescence. Suitable for human samples.
- Tried and trusted by researchers since 2005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NANOG aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9S0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
- Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
- Pathways
Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
- Associated diseases and disorders
Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab21624)
ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
Western blot - Anti-Nanog antibody (ab21624)
All lanes: Western blot - Anti-Nanog antibody (ab21624) at 1/200 dilution
Lane 1: Human fetal fibroblast lysate
Lane 2: Human iPS cell lysate
Predicted band size: 34 kDa
Western blot - Anti-Nanog antibody (ab21624)
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.
All lanes: Western blot - Anti-Nanog antibody (ab21624) at 1/1000 dilution
Lane 1: whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2: whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size: 34 kDa
Observed band size: 38 kDa
Image courtesy of Mick WauchopeImmunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab21624)
Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.
Sandwich ELISA - Anti-Nanog antibody (ab21624)
Standard Curve for Nanog (Analyte: Nanog protein (Recombinant Human Nanog protein ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
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