Rabbit Polyclonal NANOG antibody. Suitable for WB and reacts with Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human NANOG.
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- Protein & cell 14:477-4962023The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhan Chen,Jiansen Lu,Yanwen Xu,Yaping Huang,Dazhuang Wang,Peiling Liang,Shaofang Ren,Xuesong Hu,Yewen Qin,Wei Ke,Ralf Jauch,Andrew Paul Hutchins,Mei Wang,Fuchou Tang,Xiao-Yang ZhaoPubMed 36921016
- Genome biology 24:252023H3.3 contributes to chromatin accessibility and transcription factor binding at promoter-proximal regulatory elements in embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanuel Tafessu,Ryan O'Hara,Sara Martire,Altair L Dube,Purbita Saha,Vincent U Gant,Laura A BanaszynskiPubMed 36782260
- Frontiers in cell and developmental biology 10:9487782022Blastomere aggregation using phytohemagglutinin-L improves the establishment efficiency of porcine parthenogenesis-derived embryonic stem-like cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoohyeong Lee,Lian Cai,Mirae Kim,Hyerin Choi,Dongjin Oh,Ali Jawad,Eunsong Lee,Sang-Hwan HyunPubMed 36158223
- Oncology letters 20:794-8022020SATB2 knockdown decreases hypoxia-induced autophagy and stemness in oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeijie Dong et. al.PubMed 32566006
- Molecular cell 71:244-255.e52018Mettl1/Wdr4-Mediated mG tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuibin Lin et. al.PubMed 29983320
- Cell stem cell 22:851-864.e52018An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Du et. al.PubMed 29804889
- Molecular therapy : the journal of the American So 23:952-9632015cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley L Fritz et. al.PubMed 25666918
- Molecular reproduction and development 80:849-612013Expression dynamics of pluripotency genes in chicken primordial germ cells before and after colonization of the genital ridges.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohsen Naeemipour,Hesam Dehghani,Mohammadreza Bassami,Ahmadreza BahramiPubMed 23877993
- The Journal of biological chemistry 286:24685-932011Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes.
- PloS one 6:e171572011An unexpected role for the clock protein timeless in developmental apoptosis.Applications:WBReactive species:MouseLinda P O'Reilly,Simon C Watkins,Thomas E SmithgallPubMed 21359199
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NANOG. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9S0
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Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 - 1/10000 | Notes - |
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Target data
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Alternative names
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Recommended products
Rabbit Polyclonal NANOG antibody. Suitable for WB and reacts with Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human NANOG.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NANOG. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9S0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
- Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
- Pathways
Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
- Associated diseases and disorders
Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Nanog antibody (ab70482)
All lanes: Western blot - Anti-Nanog antibody (ab70482) at 0.1 µg/mL
Lane 1: Whole cell lysate from mouse embryonic stem cells at 15 µg
Lane 2: Whole cell lysate from mouse embryonic stem cells at 50 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 39 kDa
Exposure time: 12s
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Nanog antibody (ab70482)
Blocking Step: 3% BSA for 1 hour at 20°C
All lanes: Western blot - Anti-Nanog antibody (ab70482) at 1/1000 dilution
Lane 1: Murine Embryonic Fibroblasts (MEFs) at 50 µg
Lane 2: Murine Embryonic Stem (ES) Cells at 50 µg
SecondaryAll lanes: An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 40 kDa
Exposure time: 1min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Nanog antibody (ab70482)
Nanog western blot using anti-Nanog antibody ab70482. Publication image and figure legend from O'Reilly, L. P., Watkins, S. C., et al., 2011, PLoS One, PubMed 21359199.
ab70482 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70482 please see the product overview.
Knockdown of Tim suppresses spontaneous differentiation of mES cells.A, Representative images of control and Tim knockdown ES cell lines 87-22 and 89-18 after 24 and 48 h in culture. Note that spontaneously differentiating mES cell clusters are readily apparent in control ES cell cultures by 48 h (flat colonies with ragged edges; “d”) but are absent from the Tim knockdown cultures. B, Analysis of self-renewal and differentiation marker expression in Tim knockdown cell lines. Expression levels of the self-renewal markers Oct4, Sox2, Nanog, KLF4, the differentiation marker AFP, as well as Tim were assessed by quantitative immunoblot (LI-COR Odyssey infrared imaging system) of cell lysates from parental ES cells as well as the Tim knockdown ES cell lines 87-22 and 89-18. Actin immunoblots served as loading control. Immunoblots were performed in triplicate and the level of each protein was normalized to actin and is shown in the bargraph as the mean ± S.E.M. Sox2 expression levels were significantly increased in the Tim knockdown cells relative to parental ES cells (p<0.05), while AFP showed a statistically significant decrease (p<0.05). While small increases in Oct4 expression were also observed in the Tim knockdown cell lines, these changes were not statistically significant.
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