AB70482

Anti-Nanog antibody

Name: Anti-Nanog antibody (ab70482) | Abcam
Brand: Abcam Limited
SKU: AB70482
Price: 585 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(13 Publications)

Rabbit Polyclonal NANOG antibody. Suitable for WB and reacts with Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human NANOG.

View Alternative Names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

3 Images

AbReview19815****

Western blot - Anti-Nanog antibody (AB70482)

Blocking Step : 3% BSA for 1 hour at 20°C

All lanes:

Western blot - Anti-Nanog antibody (ab70482) at 1/1000 dilution

Lane 1:

Murine Embryonic Fibroblasts (MEFs) at 50 µg

Lane 2:

Murine Embryonic Stem (ES) Cells at 50 µg

Secondary

All lanes:

An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 40 kDa

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Exposure time: 1min

This image is courtesy of an anonymous Abreview

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Western blot - Anti-Nanog antibody (AB70482)

All lanes:

Western blot - Anti-Nanog antibody (ab70482) at 0.1 µg/mL

Lane 1:

Whole cell lysate from mouse embryonic stem cells at 15 µg

Lane 2:

Whole cell lysate from mouse embryonic stem cells at 50 µg

Predicted band size: 34 kDa

Observed band size: 39 kDa

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Exposure time: 12s

CiteAb

Western blot - Anti-Nanog antibody (AB70482)

Nanog western blot using anti-Nanog antibody ab70482. Publication image and figure legend from O'Reilly, L. P., Watkins, S. C., et al., 2011, PLoS One, PubMed 21359199.

ab70482 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70482 please see the product overview.

Knockdown of Tim suppresses spontaneous differentiation of mES cells.A, Representative images of control and Tim knockdown ES cell lines 87-22 and 89-18 after 24 and 48 h in culture. Note that spontaneously differentiating mES cell clusters are readily apparent in control ES cell cultures by 48 h (flat colonies with ragged edges; "d") but are absent from the Tim knockdown cultures. B, Analysis of self-renewal and differentiation marker expression in Tim knockdown cell lines. Expression levels of the self-renewal markers Oct4, Sox2, Nanog, KLF4, the differentiation marker AFP, as well as Tim were assessed by quantitative immunoblot (LI-COR Odyssey infrared imaging system) of cell lysates from parental ES cells as well as the Tim knockdown ES cell lines 87-22 and 89-18. Actin immunoblots served as loading control. Immunoblots were performed in triplicate and the level of each protein was normalized to actin and is shown in the bargraph as the mean ± S.E.M. Sox2 expression levels were significantly increased in the Tim knockdown cells relative to parental ES cells (p<0.05), while AFP showed a statistically significant decrease (p<0.05). While small increases in Oct4 expression were also observed in the Tim knockdown cell lines, these changes were not statistically significant.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human NANOG. The exact immunogen used to generate this antibody is proprietary information.

Q9H9S0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.

Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed : 25825768). Able to autorepress its expression in differentiating (ES) cells : binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

See full target information NANOG

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

FEBS open bio 14:1087-1100 PubMed38720471

2024

HELQ deficiency impairs the induction of primordial germ cell-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wan,Yaping Huang,Xingguo Xue,Gang Chang,Mei Wang,Xiao-Yang Zhao,Fang Luo,Zhi-Zhong Tang

Protein & cell 14:477-496 PubMed36921016

2023

The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Chen,Jiansen Lu,Yanwen Xu,Yaping Huang,Dazhuang Wang,Peiling Liang,Shaofang Ren,Xuesong Hu,Yewen Qin,Wei Ke,Ralf Jauch,Andrew Paul Hutchins,Mei Wang,Fuchou Tang,Xiao-Yang Zhao

Genome biology 24:25 PubMed36782260

2023

H3.3 contributes to chromatin accessibility and transcription factor binding at promoter-proximal regulatory elements in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amanuel Tafessu,Ryan O'Hara,Sara Martire,Altair L Dube,Purbita Saha,Vincent U Gant,Laura A Banaszynski

Frontiers in cell and developmental biology 10:948778 PubMed36158223

2022

Blastomere aggregation using phytohemagglutinin-L improves the establishment efficiency of porcine parthenogenesis-derived embryonic stem-like cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Joohyeong Lee,Lian Cai,Mirae Kim,Hyerin Choi,Dongjin Oh,Ali Jawad,Eunsong Lee,Sang-Hwan Hyun

Oncology letters 20:794-802 PubMed32566006

2020

SATB2 knockdown decreases hypoxia-induced autophagy and stemness in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weijie Dong,Yawen Chen,Naiying Qian,Guoqi Sima,Jianming Zhang,Zhiqin Guo,Changlin Wang

Molecular cell 71:244-255.e5 PubMed29983320

2018

Mettl1/Wdr4-Mediated mG tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuibin Lin,Qi Liu,Victor S Lelyveld,Junho Choe,Jack W Szostak,Richard I Gregory

Cell stem cell 22:851-864.e5 PubMed29804889

2018

An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Du,Mehdi Pirouz,Jiho Choi,Aaron J Huebner,Kendell Clement,Alexander Meissner,Konrad Hochedlinger,Richard I Gregory

Molecular therapy : the journal of the American So 23:952-963 PubMed25666918

2015

cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley L Fritz,Maroof M Adil,Sunnie R Mao,David V Schaffer

Molecular reproduction and development 80:849-61 PubMed23877993

2013

Expression dynamics of pluripotency genes in chicken primordial germ cells before and after colonization of the genital ridges.

Applications

Unspecified application

Species

Unspecified reactive species

Mohsen Naeemipour,Hesam Dehghani,Mohammadreza Bassami,Ahmadreza Bahrami

The Journal of biological chemistry 286:24685-93 PubMed21610077

2011

Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes.

Applications

Species

Mouse

Shannon Morey Kinney,Hang Gyeong Chin,Romualdas Vaisvila,Jurate Bitinaite,Yu Zheng,Pierre-Olivier Estève,Suhua Feng,Hume Stroud,Steven E Jacobsen,Sriharsa Pradhan

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