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AB80892

Anti-Nanog antibody

4

(18 Reviews)

|

(426 Publications)

Anti-Nanog antibody (ab80892) is a rabbit polyclonal antibody detecting Nanog in Western Blot, Flow Cytometry, IHC-P, IHC- Wmt, ICC/IF. Suitable for Mouse.

- Over 360 publications
- Trusted since 2009

View Alternative Names

Ecat4, Enk, Nanog, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC - Wmt, IHC-P, ICC, Flow Cyt, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product details

What is this antibody validated in?
Anti-Nanog antibody (ab80892) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC - Wmt), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-Nanog (ab80892) was first used in a scientific publication in 2009 and has been cited over 360 times in peer-reviewed journals.

Reviewed by scientists
Anti-Nanog (ab80892) has over 15 independent reviews from customers.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/Β-Catenin and TGF-Β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/Β-Catenin pathway Nanog works alongside proteins like Β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-Β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal (PubMed : 25825768). Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (By similarity). Able to autorepress its expression in differentiating (ES) cells : binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
See full target information Nanog

Publications (426)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1640986 PubMed40874021

2025

Chloroquine mitigates long-term effects of in vitro culture in mouse embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Kaname Sato,Itsuki Koide,Md Wasim Bari,Satoshi Kishigami

Nature communications 16:7071 PubMed40750602

2025

USP17L promotes the 2-cell-like program through deubiquitination of H2AK119ub1 and ZSCAN4.

Applications

Unspecified application

Species

Unspecified reactive species

Panpan Shi,Xukun Lu,Kairang Jin,Linlin Liu,Guoxing Yin,Wenying Wang,Jiao Yang,Lijuan Wang,Lijun Dong,Wei Xie,Lin Liu

Scientific reports 15:26163 PubMed40681589

2025

Regulation of autophagy and its role in late preimplantation during mouse embryo development.

Applications

Unspecified application

Species

Unspecified reactive species

Kei Uechi,Itsuki Koide,Saya Kanie,Tadashi Yamazaki,Satoshi Kishigami

Nucleic acids research 53: PubMed40521666

2025

Nat10 maintains the homeostasis of pluripotent and 2-cell-like states in mouse embryonic stem cells through mRNA cytidine acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Guofeng Feng,Guoxing Yin,Yusheng Liu,Chang Liu,Jie Li,Jiangtao Lu,Yongqin Yu,Ziyi Jin,Yiwei Wu,Yanbin Yue,Xiufang Gao,Jiaqiang Wang,Falong Lu,Lin Liu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70723 PubMed40497397

2025

Tmx2 Maintains Mitochondrial Function to Support Preimplantation Embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shangrong Zhang,Qing Liu,Siyu Wang,Xiaoyu Zhang,Dingmei Qin,Ruisong Bai,Ji Chen,Zijian Ma,Zhipeng Lin,Yuheng Bi,Huan Liu,Aoxue Sun,Zhongzhi Mo,Hongcheng Wang,Xiaoqing Wu,Yong Liu

World journal of clinical pediatrics 14:101080 PubMed40491730

2025

They will be famous: Multipotent stem cells in breast milk.

Applications

Unspecified application

Species

Unspecified reactive species

Gavino Faa,Giuseppina Pichiri,Pierpaolo Coni,Angelica Dessì,Matteo Fraschini,Vassilios Fanos

Scientific reports 15:14675 PubMed40287470

2025

Substituents introduction of methyl and methoxy functional groups on resveratrol stabilizes mTOR binding for autophagic cell death induction.

Applications

Unspecified application

Species

Unspecified reactive species

Zin Zin Ei,Satapat Racha,Preedakorn Chunhacha,Masashi Yokoya,Sohsuke Moriue,Hongbin Zou,Pithi Chanvorachote

iScience 28:111671 PubMed39868030

2025

Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Weberling,Dylan Siriwardena,Christopher Penfold,Neophytos Christodoulou,Thorsten E Boroviak,Magdalena Zernicka-Goetz

Veterinary sciences 12: PubMed39852882

2025

Anti-Stemness and Anti-Proliferative Effects of Cadmium on Bovine Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Penggang Liu,Xueli Chen,Yuqing Zhao,Waseem Ali,Tianle Xu,Jing Sun,Zongping Liu

Molecular therapy : the journal of the American Society of Gene Therapy 33:1213-1225 PubMed39797398

2025

Scalable control of stem cell fate by riboswitch-regulated RNA viral vector without genomic integration.

Applications

Unspecified application

Species

Unspecified reactive species

Narae Kim,Yohei Yokobayashi
View all publications

Product promise

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