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Rabbit Recombinant Monoclonal NANOG antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (AB251409), expandable thumbnail
  • Immunoprecipitation - Anti-Nanog antibody [EPR19646] - BSA and Azide free (AB251409), expandable thumbnail
  • Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (AB251409), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Alternative names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

Recommended products

Rabbit Recombinant Monoclonal NANOG antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR19646
Specificity

This antibody detects both phosphorylated and unphosphorylated Nangog.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251409 is the carrier-free version of Anti-Nanog antibody [EPR19646] ab203919.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.

Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Associated diseases and disorders

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409), expandable thumbnail

    Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)

    This data was developed using Anti-Nanog antibody [EPR19646] ab203919, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

    Negative control: NIH/3T3 (PMID: 12787505).

    All lanes: Western blot - Anti-Nanog antibody [EPR19646] (Anti-Nanog antibody [EPR19646] ab203919) at 1/1000 dilution

    Lane 1: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409), expandable thumbnail

    Immunoprecipitation - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)

    This data was developed using Anti-Nanog antibody [EPR19646] ab203919, the same antibody clone in a different buffer formulation.Nanog was immunoprecipitated from 0.35 mg of F9 (Mouse embryonic testicular cancer cell line) whole cell lysate with Anti-Nanog antibody [EPR19646] ab203919 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-Nanog antibody [EPR19646] ab203919 at 1/500 dilution. VeriBlot for IP Detection Reaction (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution. Lane 1: F9 whole cell lysate 10µg (Input). Lane 2: Anti-Nanog antibody [EPR19646] ab203919 IP in F9 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Nanog antibody [EPR19646] ab203919 in F9 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Nanog antibody [EPR19646] (Anti-Nanog antibody [EPR19646] ab203919)

    Predicted band size: 34 kDa

  • Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409), expandable thumbnail

    Western blot - Anti-Nanog antibody [EPR19646] - BSA and Azide free (ab251409)

    This data was developed using Anti-Nanog antibody [EPR19646] ab203919, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Nanog is expressed in embryonic stem cells, but not in in many somatic organs, fibroblast, lymphoid cells, and hematopoietic cell lines.

    Negative control: Jurkat (PMID: 12787505).

    All lanes: Western blot - Anti-Nanog antibody [EPR19646] (Anti-Nanog antibody [EPR19646] ab203919) at 1/1000 dilution

    Lane 1: NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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