Rabbit Recombinant Monoclonal NANOG antibody. Carrier free. Suitable for ChIP-seq, IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 15 publications.
Images
![Immunoprecipitation - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunoprecipitation-img142345.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23816.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129944.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115359.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117562.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (AB218524)")
Publications
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- Oncology reports 44:1627-16372020MicroRNA‑137 exerts protective effects on hypoxia‑induced cell injury by inhibiting autophagy/mitophagy and maintaining mitochondrial function in breast cancer stem‑like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiongying Hu et. al.PubMed 32945512
- Cell death & disease 11:5422020CircAGFG1 drives metastasis and stemness in colorectal cancer by modulating YY1/CTNNB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhang et. al.PubMed 32681092
- Cell death & disease 11:3582020CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangke He et. al.PubMed 32398664
- Journal of cellular biochemistry 121:3286-32972020Exosomes from CD133 cells carrying circ-ABCC1 mediate cell stemness and metastasis in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Zhao,Shixia Chen,Qiang FuPubMed 31960989
- Acta biomaterialia 102:220-2302019Modular design of a tissue engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinkyu Park,Christopher W Anderson,Lorenzo R Sewanan,Mehmet H Kural,Yan Huang,Jiesi Luo,Liqiong Gui,Muhammad Riaz,Colleen A Lopez,Ronald Ng,Subhash K Das,Juan Wang,Laura Niklason,Stuart G Campbell,Yibing QyangPubMed 31634626
- IUBMB life 72:285-2952019Silencing transcription factor FOXM1 represses proliferation, migration, and invasion while inducing apoptosis of liver cancer stem cells by regulating the expression of ALDH2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijian Chen,Meiyun Wu,Chunyi Ji,Miaoxian Yuan,Chaoyang Liu,Qiang YinPubMed 31580537
- International journal of oncology 55:845-8592019Hypoxia‑induced internalization of connexin 26 and connexin 43 in pulmonary epithelial cells is involved in the occurrence of non‑small cell lung cancer via the P53/MDM2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShang-Gan Zeng et. al.PubMed 31485592
- Journal of cellular physiology 235:1051-10642019MFN2 silencing promotes neural differentiation of embryonic stem cells via the Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiqi Yi,Chenghao Cui,Xiaotian Huang,Xiaohui Yin,Yang Li,Jinhua Wen,Qingxian LuanPubMed 31276200
- Proceedings of the National Academy of Sciences of 112:E2337-462015Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Yang et. al.PubMed 25870291
- Journal of virology 88:11315-262014High-risk human papillomavirus E6 protein promotes reprogramming of Fanconi anemia patient cells through repression of p53 but does not allow for sustained growth of induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy M Chlon,Elizabeth E Hoskins,Christopher N Mayhew,Kathryn A Wikenheiser-Brokamp,Stella M Davies,Parinda Mehta,Kasiani C Myers,James M Wells,Susanne I WellsPubMed 25031356
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP-seq | IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Tested |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
12 products for Alternative Product
2 products for Alternative Version
Target data
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Alternative names
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Recommended products
Rabbit Recombinant Monoclonal NANOG antibody. Carrier free. Suitable for ChIP-seq, IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2027(2)
- Purification technique
- Affinity purification Protein A
- Specificity
100% identities with NANOGP8
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab218524 is the carrier-free version of Anti-Nanog antibody [EPR2027(2)] ab109250.
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
- Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
- Pathways
Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
- Associated diseases and disorders
Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Immunoprecipitation - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunoprecipitation-img142345.jpg "Immunoprecipitation - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunoprecipitation - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Anti-Nanog antibody [EPR2027(2)] ab109250 (purified) at 1/40 dilution (1.5 μg/ml) immunoprecipitating Nanog in NCCIT whole cell lysate.
Lane 1 (input): NCCIT(Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): Anti-Nanog antibody [EPR2027(2)] ab109250 & NCCIT whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Nanog antibody [EPR2027(2)] ab109250 in NCCIT whole cell lysateFor western blotting, Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/500 dilution (1.5 μg/ml) VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-Nanog antibody [EPR2027(2)] (Anti-Nanog antibody [EPR2027(2)] ab109250)
Predicted band size: 34 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23816.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human seminoma tissue labelling Nanog with purified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129944.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human seminoma tissue labelling Nanog with unpurified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115359.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human dysgerminoma tissue labelling Nanog with unpurified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117562.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human embryonal carcinoma tissue labelling Nanog with unpurified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134304.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human normal colon tissue shows negative staining of Nanog with unpurified Anti-Nanog antibody [EPR2027(2)] ab109250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80941.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human adult kidney tissue shows negative staining of Nanog with unpurified Anti-Nanog antibody [EPR2027(2)] ab109250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--flow-cytometry-intracellular-img38070.jpg "Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Intracellular Flow Cytometry analysis of NCCIT cells labelling Nanog with purified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/70 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunocytochemistry-immunofluorescence-img28416.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunocytochemistry/Immunofluorescence analysis of Human Liver cells labelling Nanog with unpurified Anti-Nanog antibody [EPR2027(2)] ab109250. Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 1% BSA for 12 hours at 4°C. Sample was incubated with primary antibody (1/500 in PBS) for 16 hour at 4°C. An Alexa Fluor®647-conjugated Donkey anti-rabbit(1/1000) IgG polyclonal was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunocytochemistry-immunofluorescence-img26336.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunocytochemistry/Immunofluorescence analysis of embryonic carcinoma cells labelling Nanog with unpurified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunocytochemistry-immunofluorescence-img17739.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Immunocytochemistry/Immunofluorescence analysis of NCCIT(human pluripotent embryonal carcinoma) cells labelling Nanog with purified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![ChIP-sequencing - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--chip-sequencing-img402752.jpg "ChIP-sequencing - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
ChIP-sequencing - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)
Chromatin was prepared from NCCIT (Human pluripotent embryonic carcinoma cell line) cells. ChIP was performed with 10^7 NCCIT cells and 8 µg of Anti-Nanog antibody [EPR2027(2)] ab109250 [[EPR2027(2)]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nanog antibody [EPR2027(2)] ab109250).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134304.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80941.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
![Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--flow-cytometry-intracellular-img38070.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunocytochemistry-immunofluorescence-img28416.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunocytochemistry-immunofluorescence-img26336.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--immunocytochemistry-immunofluorescence-img17739.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
![ChIP-sequencing - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-epr20272-bsa-and-azide-free-ab218524--chip-sequencing-img402752.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-Nanog antibody [EPR2027(2)] - BSA and Azide free (ab218524)")
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