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AB62734

Anti-Nanog antibody [NNG-811]

5

(3 Reviews)

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(48 Publications)

Anti-Nanog antibody [NNG-811] (ab62734) is a mouse monoclonal antibody detecting Nanog in Western Blot, IP, ICC/IF. Suitable for Human.

- Over 40 publications
- Trusted since 2008

View Alternative Names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (AB62734)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (AB62734)

3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 μg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.

Immunoprecipitation - Anti-Nanog antibody [NNG-811] (AB62734)
  • IP

Supplier Data

Immunoprecipitation - Anti-Nanog antibody [NNG-811] (AB62734)

Nanog was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract using ab62734.

Lane 1 : 5 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 2 : 10 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 3 : Negative control without cell extract.
Lane 4 : NT2/D1 whole cell extract (input).

All lanes:

Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)

Predicted band size: 34 kDa

false

Western blot - Anti-Nanog antibody [NNG-811] (AB62734)
  • WB

Unknown

Western blot - Anti-Nanog antibody [NNG-811] (AB62734)

Lane 1:

Western blot - Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/mL

Lane 2:

Western blot - Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/mL

Lane 3:

Negative control – no primary antibody

All lanes:

Cell extract of NT2 cells at 20 µg

Secondary

All lanes:

Goat Anti-Mouse IgG

Predicted band size: 34 kDa

Observed band size: 40 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NNG-811

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NANOG.

Q9H9S0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Nanog antibody [NNG-811] (ab62734) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Nanog?
Anti-Nanog [NNG-811] (ab62734) specifically detects a band for Nanog (UniProt: Q9H9S0) at a molecular weight of 35kDa.

Trusted by the scientific community
Anti-Nanog [NNG-811] (ab62734) was first used in a scientific publication in 2008 and has been cited over 40 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
Purified Immunoglobulin
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/Β-Catenin and TGF-Β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/Β-Catenin pathway Nanog works alongside proteins like Β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-Β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed : 25825768). Able to autorepress its expression in differentiating (ES) cells : binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
See full target information NANOG
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Publications (48)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2924:131-143 PubMed40307640

2025

Toxicity Testing Using Organoids Made from Human-Induced Pluripotent Stem Cells Engineered to Report Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mona Elhendawi,Melanie L Lawrence,Jamie A Davies

Journal of Huntington's disease 13:41-53 PubMed38427495

2024

Mono- and Biallelic Inactivation of Huntingtin Gene in Patient-Specific Induced Pluripotent Stem Cells Reveal HTT Roles in Striatal Development and Neuronal Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Morgane Louessard,Michel Cailleret,Margot Jarrige,Julie Bigarreau,Sophie Lenoir,Noëlle Dufour,Maria Rey,Frédéric Saudou,Nicole Deglon,Anselme L Perrier

Advanced biology 8:e2300276 PubMed37675827

2023

Human IPSC-Derived PreBötC-Like Neurons and Development of an Opiate Overdose and Recovery Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufang Guo,Nesar Akanda,Gabriella Fiorino,Siddharth Nimbalkar,Christopher J Long,Alisha Colón,Aakash Patel,Patrick J Tighe,James J Hickman

Annals of clinical and translational neurology 10:1239-1253 PubMed37283238

2023

Engineered extracellular matrices facilitate brain organoids from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ayşe J Muñiz,Tuğba Topal,Michael D Brooks,Angela Sze,Do Hoon Kim,Jacob Jordahl,Joe Nguyen,Paul H Krebsbach,Masha G Savelieff,Eva L Feldman,Joerg Lahann

Cellular and molecular gastroenterology and hepatology 15:741-764 PubMed36521659

2022

Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn's Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Rizzo,Federica Rubbino,Sudharshan Elangovan,Giusy Sammarco,Sara Lovisa,Silvia Restelli,Samuel Elias Pineda Chavez,Luca Massimino,Luigi Lamparelli,Marianna Paulis,Annalisa Maroli,Giulia Roda,Mohammad Shalaby,Michele Carvello,Caterina Foppa,Sheona P Drummond,Paola Spaggiari,Federica Ungaro,Antonino Spinelli,Alberto Malesci,Alessandro Repici,Anthony J Day,Alessandro Armuzzi,Silvio Danese,Stefania Vetrano

Biomedicines 10: PubMed36551899

2022

Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel A Oldershaw,Gavin Richardson,Phillippa Carling,W Andrew Owens,David J Lundy,Annette Meeson

Annals of plastic surgery 89:e60-e68 PubMed36416705

2022

High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction.

Applications

Unspecified application

Species

Unspecified reactive species

Eyal Shapira,Lori Plonski,Shaked Menashe,Andre Ofek,Adaya Rosenthal,Massimiliano Brambilla,Gary Goldenberg,Sahar Haimowitz,Lior Heller

Advanced therapeutics 5: PubMed36589922

2022

An induced pluripotent stem cell-derived NMJ platform for study of the NGLY1-Congenital Disorder of Deglycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Trevor Sasserath,Ashley L Robertson,Roxana Mendez,Tristan T Hays,Ethan Smith,Helena Cooper,Nesar Akanda,John W Rumsey,Xiufang Guo,Atena Farkhondeh,Manisha Pradhan,Karsten Baumgaertel,Matthew Might,Steven Rodems,Wei Zheng,James J Hickman

iScience 25:103884 PubMed35243244

2022

Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

M L Lawrence,M Elhendawi,M Morlock,W Liu,S Liu,A Palakkan,L F Seidl,P Hohenstein,A K Sjögren,J A Davies

Aging 13:24101-24116 PubMed34739394

2021

Curcumol inhibits malignant biological behaviors and TMZ-resistance in glioma cells by inhibiting long noncoding RNA FOXD2-As1-promoted EZH2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyang Lv,Jiangchuan Sun,Linfeng Hu,Ying Qian,Chunlei Fan,Nan Tian
View all publications
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