Mouse Monoclonal NANOG antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Transfected cell line - Human samples. Cited in 44 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NANOG.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (AB62734), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-nng-811-ab62734--immunocytochemistry-immunofluorescence-img45213.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (AB62734)")
![Immunoprecipitation - Anti-Nanog antibody [NNG-811] (AB62734), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-nng-811-ab62734--immunoprecipitation-img128434.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Nanog antibody [NNG-811] (AB62734)")
![Western blot - Anti-Nanog antibody [NNG-811] (AB62734), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-nng-811-ab62734--western-blot-img64234.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nanog antibody [NNG-811] (AB62734)")
Publications
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- Cellular and molecular gastroenterology and hepatology 15:741-7642022Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn's Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Rizzo,Federica Rubbino,Sudharshan Elangovan,Giusy Sammarco,Sara Lovisa,Silvia Restelli,Samuel Elias Pineda Chavez,Luca Massimino,Luigi Lamparelli,Marianna Paulis,Annalisa Maroli,Giulia Roda,Mohammad Shalaby,Michele Carvello,Caterina Foppa,Sheona P Drummond,Paola Spaggiari,Federica Ungaro,Antonino Spinelli,Alberto Malesci,Alessandro Repici,Anthony J Day,Alessandro Armuzzi,Silvio Danese,Stefania VetranoPubMed 36521659
- Biomedicines 10:2022Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel A Oldershaw,Gavin Richardson,Phillippa Carling,W Andrew Owens,David J Lundy,Annette MeesonPubMed 36551899
- Annals of plastic surgery 89:e60-e682022High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEyal Shapira,Lori Plonski,Shaked Menashe,Andre Ofek,Adaya Rosenthal,Massimiliano Brambilla,Gary Goldenberg,Sahar Haimowitz,Lior HellerPubMed 36416705
- Advanced therapeutics 5:2022An induced pluripotent stem cell-derived NMJ platform for study of the NGLY1-Congenital Disorder of Deglycosylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrevor Sasserath,Ashley L Robertson,Roxana Mendez,Tristan T Hays,Ethan Smith,Helena Cooper,Nesar Akanda,John W Rumsey,Xiufang Guo,Atena Farkhondeh,Manisha Pradhan,Karsten Baumgaertel,Matthew Might,Steven Rodems,Wei Zheng,James J HickmanPubMed 36589922
- iScience 25:1038842022Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM L Lawrence,M Elhendawi,M Morlock,W Liu,S Liu,A Palakkan,L F Seidl,P Hohenstein,A K Sjögren,J A DaviesPubMed 35243244
- Aging 13:24101-241162021Curcumol inhibits malignant biological behaviors and TMZ-resistance in glioma cells by inhibiting long noncoding RNA FOXD2-As1-promoted EZH2 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuyang Lv,Jiangchuan Sun,Linfeng Hu,Ying Qian,Chunlei Fan,Nan TianPubMed 34739394
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e21023542021NOCICEPTRA: Gene and microRNA Signatures and Their Trajectories Characterizing Human iPSC-Derived Nociceptor Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaximilian Zeidler,Kai K Kummer,Clemens L Schöpf,Theodora Kalpachidou,Georg Kern,M Zameel Cader,Michaela KressPubMed 34486248
- PloS one 16:e02562802021Colon adenocarcinoma-derived cells possessing stem cell function can be modulated using renin-angiotensin system inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew J Munro,Lifeng Peng,Susrutha K Wickremesekera,Swee T TanPubMed 34428252
- Journal of personalized medicine 10:2020A Human Induced Pluripotent Stem Cell-Derived Isogenic Model of Huntington's Disease Based on Neuronal Cells Has Several Relevant Phenotypic Abnormalities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTuyana Malankhanova et. al.PubMed 33182269
- Cell reports 32:1080902020Myosin-18B Regulates Higher-Order Organization of the Cardiac Sarcomere through Thin Filament Cross-Linking and Thick Filament Dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharissa L Latham,Nadine Weiß,Kristin Schwanke,Claudia Thiel,David R Croucher,Robert Zweigerdt,Dietmar J Manstein,Manuel H TaftPubMed 32877672
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NANOG. Database link Q9H9S0
Abcam Recommends
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Transfected cell line - Human | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Alternative names
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Recommended products
Mouse Monoclonal NANOG antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Transfected cell line - Human samples. Cited in 44 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NANOG.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NANOG. Database link Q9H9S0
- Clone number
- NNG-811
- Purity
- IgG fraction
- Concentration
- Purification notes
Purified Immunoglobulin
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
- Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
- Pathways
Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
- Associated diseases and disorders
Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-nng-811-ab62734--immunocytochemistry-immunofluorescence-img45213.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734)")
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [NNG-811] (ab62734)
3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 μg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
![Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-nng-811-ab62734--immunoprecipitation-img128434.jpg "Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)")
Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)
Nanog was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract using ab62734.
Lane 1: 5 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 2: 10 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 3: Negative control without cell extract.
Lane 4: NT2/D1 whole cell extract (input).All lanes: Immunoprecipitation - Anti-Nanog antibody [NNG-811] (ab62734)
Predicted band size: 34 kDa
![Western blot - Anti-Nanog antibody [NNG-811] (ab62734), expandable thumbnail](https://content.abcam.com/products/images/nanog-antibody-nng-811-ab62734--western-blot-img64234.jpg "Western blot - Anti-Nanog antibody [NNG-811] (ab62734)")
Western blot - Anti-Nanog antibody [NNG-811] (ab62734)
Lane 1: Western blot - Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/mL
Lane 2: Western blot - Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/mL
Lane 3: Negative control – no primary antibody
All lanes: Cell extract of NT2 cells at 20 µg
SecondaryAll lanes: Goat Anti-Mouse IgG
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 40 kDa
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