JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB70000

Anti-NANOS2 antibody

1

(1 Review)

|

(4 Publications)

Rabbit Polyclonal NANOS2 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NANOS2.

View Alternative Names

NOS2, NANOS2, Nanos homolog 2, NOS-2

1 Images
Western blot - Anti-NANOS2 antibody (AB70000)
  • WB

Unknown

Western blot - Anti-NANOS2 antibody (AB70000)

All lanes:

Western blot - Anti-NANOS2 antibody (ab70000) at 2 µg/mL

All lanes:

SK-N-SH cell lysate at 15 µg

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NANOS2. The exact immunogen used to generate this antibody is proprietary information.

P60321

Specificity

ab70000 does not cross-react with either Nanos1 or Nanos3.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p>Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Product details

Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NANOS2 protein also known simply as NANOS 2 is a member of the NANOS family of proteins and plays an integral role in the regulation of germ cell development. It has a molecular mass of approximately 17 kDa. This protein is primarily expressed in the male germline specifically in spermatogonial stem cells within the testes. Its expression is tightly regulated and is essential for the proper maintenance and differentiation of these stem cells contributing significantly to male fertility.
Biological function summary

NANOS2 acts as a post-transcriptional regulator and it is part of a ribonucleoprotein complex. It helps maintain germ cell pluripotency and influences the cell cycle. Functioning mainly through the binding to the 3' untranslated region (3' UTR) of target mRNAs NANOS2 controls the translation and stability of specific mRNAs that are important for germ cell maintenance and differentiation. This control is essential for preventing premature meiosis or differentiation in male germ cells.

Pathways

The involvement of NANOS2 is critical in both the PI3K/AKT signaling pathway and the TGF-beta signaling pathway. In the PI3K/AKT pathway NANOS2 interacts with proteins such as PI3K and AKT to regulate cell survival and proliferation specifically in germ cells. In the TGF-beta pathway NANOS2 works alongside Smad proteins to modulate responses essential for cell cycle regulation ensuring the proper timing of germ cell differentiation.

Disruptions in NANOS2 expression or function are closely related to infertility particularly affecting spermatogenesis. Studies have shown that NANOS2 abnormalities can lead to male infertility due to inadequate spermatogonial stem cell maintenance and differentiation. Additionally NANOS2 has been linked to testicular germ cell tumors with its expression patterns altered in cancerous cells compared to normal tissue. Such pathological conditions often see interactions with oncogenic proteins like PLZF highlighting the importance of NANOS2 in both normal and disease states of germ cell development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).
See full target information NANOS2
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

World journal of stem cells 12:500-513 PubMed32742567

2020

Cytotoxicity of nonylphenol on spermatogonial stem cells phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Hao Lei,Wen Yan,Chun-Hua Luo,Yu-Ming Guo,Yang-Yang Zhang,Xing-Huan Wang,Xin-Jun Su

Journal of cellular biochemistry 120:1174-1184 PubMed30335884

2018

Reelin regulates male mouse reproductive capacity via the sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengru Zhuang,Bo Li,Yangxue Huang,Qijing Lei,Ruichuan Yan,Na Li,Kuldip Sidhu,Xiang Cheng,Xinrong Yan,Yiliang Miao,Shanting Zhao,Jinlian Hua

The Journal of experimental medicine 213:2209-26 PubMed27621415

2016

PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Deping Kong,Yujun Shen,Guizhu Liu,Shengkai Zuo,Yong Ji,Ankang Lu,Masataka Nakamura,Michael Lazarus,Constantine A Stratakis,Richard M Breyer,Ying Yu

Molecular human reproduction 15:165-71 PubMed19168545

2009

The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction.

Applications

IHC-P, WB

Species

Human, Human

K M Kusz,L Tomczyk,M Sajek,A Spik,A Latos-Bielenska,P Jedrzejczak,L Pawelczyk,J Jaruzelska
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com