Rabbit Recombinant Monoclonal NAP1L1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--western-blot-img115398.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67748.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061)")
![Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--immunocytochemistry-immunofluorescence-img21521.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061)")
![Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--immunoprecipitation-img72938.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061)")
![Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--flow-cytometry-intracellular-img50671.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (AB250061)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly (PubMed:20002496, PubMed:26841755). Participates also in several important DNA repair mechanisms: greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair (PubMed:28369616). Stimulates also homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair (PubMed:24798879). Plays a key role in the regulation of embryonic neurogenesis (By similarity). Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (By similarity). Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter (By similarity).(Microbial infection) Positively regulates Epstein-Barr virus reactivation in epithelial cells through the induction of viral BZLF1 expression.(Microbial infection) Together with human herpesvirus 8 protein LANA1, assists the proper assembly of the nucleosome on the replicated viral DNA.
Alternative names
Nucleosome assembly protein 1-like 1, NAP-1-related protein, hNRP, NRP, NAP1L1
Recommended products
Rabbit Recombinant Monoclonal NAP1L1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Nucleosome assembly protein 1-like 1, NAP-1-related protein, hNRP, NRP, NAP1L1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR11845
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250061 is the carrier-free version of Anti-NAP1L1 antibody [EPR11845] ab178687.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NAP1L1 also known as Nucleosome Assembly Protein 1-Like 1 plays a role mechanically by assisting in nucleosome assembly. This protein has a mass of about 45 kDa and is expressed in various tissues including the brain and liver. NAP1L1 moves within the nucleus and cytoplasm which allows interaction with DNA and other proteins involved in chromatin organization.
- Biological function summary
NAP1L1 contributes to chromatin remodeling by facilitating the assembly and disassembly of nucleosomes. NAP1L1 forms part of a complex that modulates chromatin structure and function influencing gene expression and DNA replication. It interacts with histones contributing to the deposition and removal of histones H2A and H2B making it essential for proper cell cycle progression and regulation.
- Pathways
NAP1L1 acts in key signaling interconnected with chromatin modification and cell cycle control. It participates in the histone chaperone pathways influencing chromatin structure dynamics. NAP1L1 associates with proteins like histone H2A-H2B and the chromatin assembly factor CAF-1. These proteins collectively maintain chromatin integrity and regulation during DNA replication and repair processes.
- Associated diseases and disorders
NAP1L1 has links to certain cancers and neurological disorders. Increased levels of NAP1L1 are observable in some tumor types suggesting a role in cancer progression. In neurological contexts alterations in this protein are noted in conditions like Alzheimer's disease where chromatin misregulation is apparent. Connections with other proteins such as histone variants may further implicate NAP1L1 in these pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--western-blot-img115398.jpg "Western blot - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)")
Western blot - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
This data was developed using Anti-NAP1L1 antibody [EPR11845] ab178687, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NAP1L1 antibody [EPR11845] (Anti-NAP1L1 antibody [EPR11845] ab178687) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: A431 cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
Predicted band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67748.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
This data was developed using Anti-NAP1L1 antibody [EPR11845] ab178687, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with Anti-NAP1L1 antibody [EPR11845] ab178687 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--immunocytochemistry-immunofluorescence-img21521.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)")
Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
This data was developed using Anti-NAP1L1 antibody [EPR11845] ab178687, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling NAP1L1 with Anti-NAP1L1 antibody [EPR11845] ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).
![Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--immunoprecipitation-img72938.jpg "Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)")
Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
This data was developed using Anti-NAP1L1 antibody [EPR11845] ab178687, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using Anti-NAP1L1 antibody [EPR11845] ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] (Anti-NAP1L1 antibody [EPR11845] ab178687)
Predicted band size: 45 kDa
![Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061), expandable thumbnail](https://content.abcam.com/products/images/nap1l1-antibody-epr11845-bsa-and-azide-free-ab250061--flow-cytometry-intracellular-img50671.jpg "Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)")
Flow Cytometry (Intracellular) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
This data was developed using Anti-NAP1L1 antibody [EPR11845] ab178687, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized 293T cells using Anti-NAP1L1 antibody [EPR11845] ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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