Anti-NAPE-PLD antibody

• WB

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Western blot - Anti-NAPE-PLD antibody (AB95397)

All lanes:

Western blot - Anti-NAPE-PLD antibody (ab95397) at 3 µg/mL

Lane 1:

Human cerebellum supernatant at 20 µg

Lane 2:

Mouse brain homogenate at 30 µg

Lane 3:

Mouse brain high density membrane at 30 µg

Predicted band size: 46 kDa

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• WB

CiteAb

Western blot - Anti-NAPE-PLD antibody (AB95397)

NAPE-PLD western blot using anti-NAPE-PLD antibody ab95397. Publication image and figure legend from Geurts, L., Everard, A., et al., 2015, Nat Commun, PubMed 25757720.

ab95397 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab95397 please see the product overview.

Specific deletion of Napepld in adipose tissue.(a) Representative adipose tissue immunoblot of NAPE-PLD and β-actin in WT mice and cKO mice. (b) mRNA relative expression of Napepld in different adipose tissue deposits (subcutaneous, visceral, epididymal and brown adipose tissue—SAT, VAT, EAT and BAT), as well as in liver, colon and tibialis muscle under CT diet and HFD in cKO mice and WT mice (n=20–27). These data (a,b) correspond to the results of three independent experiments. Data with '*' indicate a significant difference (p<0.05) versus WT-CT or WT-HFD according to the unpaired two-tailed Student's t-test. (c) SAT levels of AEA, PEA, OEA and SEA (pmol per g tissue) measured by HPLC-MS (n=6–10). Data are presented as the mean±s.e.m. Data with different superscript letters are significantly different (P<0.05) according to post-hoc one-way analysis of variance statistical analysis.

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