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Rabbit Polyclonal NAPRT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAPRT aa 100-450.

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Images

Western blot - Anti-NAPRT1 antibody (AB127699), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody (AB127699), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NAPRT1 antibody (AB127699), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human NAPRT aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q6XQN6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/5000.00000 - 1/20000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed:17604275, PubMed:21742010, PubMed:26042198). Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed:17604275).

Alternative names

Recommended products

Rabbit Polyclonal NAPRT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAPRT aa 100-450.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human NAPRT aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q6XQN6
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NAPRT1 also known as nicotinate phosphoribosyltransferase plays an important role in the Preiss-Handler pathway where it converts nicotinic acid (a form of vitamin B3) to nicotinic acid mononucleotide. NAPRT1 is approximately 55 kDa in size. This protein mainly expresses in liver kidney and several tissues with high metabolic rates. An accurate understanding of NAPRT1’s expression is essential for targeting its function in NAD+ biosynthesis.

Biological function summary

Nicotinate phosphoribosyltransferase participates in the biosynthesis of NAD+ an important cofactor in redox reactions. It combines with other enzymes to effectively maintain NAD+ levels in cells. NAD+ supports cellular metabolism DNA repair and longevity. NAPRT1's activity integrated with other components sustains cellular energy processes and influences cellular health.

Pathways

NAD+ biosynthesis involves multiple mechanisms where NAPRT1 holds importance. One significant pathway includes the Preiss-Handler pathway where NAPRT1 works in tandem with NMNAT1 to ensure NAD+ production. Other pathways also connect NAPRT1 with NAMPT another enzyme that converts nicotinamide to NAD+ highlighting the biochemical network sustaining NAD+ levels important for cell function.

Associated diseases and disorders

Alterations in NAPRT1 expression relate to pellagra a condition resulting from vitamin B3 deficiency. Research also links NAPRT1 to certain cancers where its expression may support tumor metabolism. NAPRT1's association with NAMPT makes it a potential biomarker for therapeutic strategies aiming to modulate NAD+ biosynthesis in pathological states.

Product promise

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3 product images

  • Western blot - Anti-NAPRT1 antibody (ab127699), expandable thumbnail

    Western blot - Anti-NAPRT1 antibody (ab127699)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-NAPRT1 antibody (ab127699) at 1/10000 dilution

    All lanes: HeLa whole cell lysate at 30 µg

    Predicted band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody (ab127699), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody (ab127699)

    ab127699, at a dilution of 1/500, staining NAPRT1 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.

  • Immunocytochemistry/ Immunofluorescence - Anti-NAPRT1 antibody (ab127699), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NAPRT1 antibody (ab127699)

    ab127699, at a dilution of 1/500, staining NAPRT1 in methanol-fixed HeLa cells by Immunofluorescence (Green). Alpha-tubulin filaments were stained Red.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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