Rabbit Polyclonal NAPRT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAPRT aa 100-450.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Select an associated product type
Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed:17604275, PubMed:21742010, PubMed:26042198). Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed:17604275).
FHIP, NAPRT1, NAPRT, Nicotinate phosphoribosyltransferase, NAPRTase, FHA-HIT-interacting protein, Nicotinate phosphoribosyltransferase domain-containing protein 1
Rabbit Polyclonal NAPRT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAPRT aa 100-450.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
NAPRT1 also known as nicotinate phosphoribosyltransferase plays an important role in the Preiss-Handler pathway where it converts nicotinic acid (a form of vitamin B3) to nicotinic acid mononucleotide. NAPRT1 is approximately 55 kDa in size. This protein mainly expresses in liver kidney and several tissues with high metabolic rates. An accurate understanding of NAPRT1’s expression is essential for targeting its function in NAD+ biosynthesis.
Nicotinate phosphoribosyltransferase participates in the biosynthesis of NAD+ an important cofactor in redox reactions. It combines with other enzymes to effectively maintain NAD+ levels in cells. NAD+ supports cellular metabolism DNA repair and longevity. NAPRT1's activity integrated with other components sustains cellular energy processes and influences cellular health.
NAD+ biosynthesis involves multiple mechanisms where NAPRT1 holds importance. One significant pathway includes the Preiss-Handler pathway where NAPRT1 works in tandem with NMNAT1 to ensure NAD+ production. Other pathways also connect NAPRT1 with NAMPT another enzyme that converts nicotinamide to NAD+ highlighting the biochemical network sustaining NAD+ levels important for cell function.
Alterations in NAPRT1 expression relate to pellagra a condition resulting from vitamin B3 deficiency. Research also links NAPRT1 to certain cancers where its expression may support tumor metabolism. NAPRT1's association with NAMPT makes it a potential biomarker for therapeutic strategies aiming to modulate NAD+ biosynthesis in pathological states.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7.5% SDS PAGE
All lanes: Western blot - Anti-NAPRT1 antibody (ab127699) at 1/10000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 58 kDa
ab127699, at a dilution of 1/500, staining NAPRT1 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
ab127699, at a dilution of 1/500, staining NAPRT1 in methanol-fixed HeLa cells by Immunofluorescence (Green). Alpha-tubulin filaments were stained Red.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com