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AB88562

Anti-NAPRT1 antibody

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Mouse Polyclonal NAPRT1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NAPRT aa 1-500.

View Alternative Names

FHIP, NAPRT1, NAPRT, Nicotinate phosphoribosyltransferase, NAPRTase, FHA-HIT-interacting protein, Nicotinate phosphoribosyltransferase domain-containing protein 1

2 Images
Western blot - Anti-NAPRT1 antibody (AB88562)
  • WB

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Western blot - Anti-NAPRT1 antibody (AB88562)

All lanes:

Western blot - Anti-NAPRT1 antibody (ab88562) at 5 µg/mL

All lanes:

human liver tissue lysate at 50 µg

Predicted band size: 58 kDa

Observed band size: 50 kDa

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Western blot - Anti-NAPRT1 antibody (AB88562)
  • WB

Unknown

Western blot - Anti-NAPRT1 antibody (AB88562)

All lanes:

Western blot - Anti-NAPRT1 antibody (ab88562) at 5 µg/mL

Lane 1:

NAPRT1 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Predicted band size: 58 kDa

Observed band size: 51 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human NAPRT aa 1-500. The exact immunogen used to generate this antibody is proprietary information.

Q6XQN6

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAPRT1 also known as nicotinate phosphoribosyltransferase plays an important role in the Preiss-Handler pathway where it converts nicotinic acid (a form of vitamin B3) to nicotinic acid mononucleotide. NAPRT1 is approximately 55 kDa in size. This protein mainly expresses in liver kidney and several tissues with high metabolic rates. An accurate understanding of NAPRT1’s expression is essential for targeting its function in NAD+ biosynthesis.
Biological function summary

Nicotinate phosphoribosyltransferase participates in the biosynthesis of NAD+ an important cofactor in redox reactions. It combines with other enzymes to effectively maintain NAD+ levels in cells. NAD+ supports cellular metabolism DNA repair and longevity. NAPRT1's activity integrated with other components sustains cellular energy processes and influences cellular health.

Pathways

NAD+ biosynthesis involves multiple mechanisms where NAPRT1 holds importance. One significant pathway includes the Preiss-Handler pathway where NAPRT1 works in tandem with NMNAT1 to ensure NAD+ production. Other pathways also connect NAPRT1 with NAMPT another enzyme that converts nicotinamide to NAD+ highlighting the biochemical network sustaining NAD+ levels important for cell function.

Alterations in NAPRT1 expression relate to pellagra a condition resulting from vitamin B3 deficiency. Research also links NAPRT1 to certain cancers where its expression may support tumor metabolism. NAPRT1's association with NAMPT makes it a potential biomarker for therapeutic strategies aiming to modulate NAD+ biosynthesis in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed : 17604275, PubMed : 21742010, PubMed : 26042198). Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed : 17604275).
See full target information NAPRT
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