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AB212028

Anti-NAPRT1 antibody [CL0366]

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(1 Publication)

Mouse Monoclonal NAPRT1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAPRT aa 250-400.

View Alternative Names

FHIP, NAPRT1, NAPRT, Nicotinate phosphoribosyltransferase, NAPRTase, FHA-HIT-interacting protein, Nicotinate phosphoribosyltransferase domain-containing protein 1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)

Immunohistochemical analysis of Human duodenum tissue labeling NAPRT1 with ab212028 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)

Immunohistochemical analysis of Human placenta tissue labeling NAPRT1 with ab212028 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)

Immunohistochemical analysis of Human testis tissue labeling NAPRT1 with ab212028 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)

Immunohistochemical analysis of Human fallopian tube tissue labeling NAPRT1 with ab212028 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0366] (AB212028)

Immunohistochemical analysis of Human kidney tissue labeling NAPRT1 with ab212028 at 1/200 dilution.

Western blot - Anti-NAPRT1 antibody [CL0366] (AB212028)
  • WB

Supplier Data

Western blot - Anti-NAPRT1 antibody [CL0366] (AB212028)

All lanes:

Western blot - Anti-NAPRT1 antibody [CL0366] (ab212028) at 1/500 dilution

All lanes:

Human liver lysate

Predicted band size: 58 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0366

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NAPRT aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q6XQN6

Epitope

epitope located within the peptide sequence IRKVFRAAAA (aa 331-340)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAPRT1 also known as nicotinate phosphoribosyltransferase plays an important role in the Preiss-Handler pathway where it converts nicotinic acid (a form of vitamin B3) to nicotinic acid mononucleotide. NAPRT1 is approximately 55 kDa in size. This protein mainly expresses in liver kidney and several tissues with high metabolic rates. An accurate understanding of NAPRT1’s expression is essential for targeting its function in NAD+ biosynthesis.
Biological function summary

Nicotinate phosphoribosyltransferase participates in the biosynthesis of NAD+ an important cofactor in redox reactions. It combines with other enzymes to effectively maintain NAD+ levels in cells. NAD+ supports cellular metabolism DNA repair and longevity. NAPRT1's activity integrated with other components sustains cellular energy processes and influences cellular health.

Pathways

NAD+ biosynthesis involves multiple mechanisms where NAPRT1 holds importance. One significant pathway includes the Preiss-Handler pathway where NAPRT1 works in tandem with NMNAT1 to ensure NAD+ production. Other pathways also connect NAPRT1 with NAMPT another enzyme that converts nicotinamide to NAD+ highlighting the biochemical network sustaining NAD+ levels important for cell function.

Alterations in NAPRT1 expression relate to pellagra a condition resulting from vitamin B3 deficiency. Research also links NAPRT1 to certain cancers where its expression may support tumor metabolism. NAPRT1's association with NAMPT makes it a potential biomarker for therapeutic strategies aiming to modulate NAD+ biosynthesis in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed : 17604275, PubMed : 21742010, PubMed : 26042198). Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed : 17604275).
See full target information NAPRT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 21:253 PubMed39385182

2024

Upregulation of keratin 15 is required for varicella-zoster virus replication in keratinocytes and is attenuated in the live attenuated vOka vaccine strain.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Tommasi,Ohad Yogev,Michael B Yee,Andriani Drousioti,Meleri Jones,Alice Ring,Manuraj Singh,Inga Dry,Oscar Atkins,Aishath S Naeem,Nisha Kriplani,Arne N Akbar,Jürgen G Haas,Edel A O'Toole,Paul R Kinchington,Judith Breuer
View all publications
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