AB175426

Anti-NAPSIN A antibody [10C4B8]

Name: Anti-NAPSIN A antibody [10C4B8] (ab175426) | Abcam
Brand: Abcam Limited
SKU: AB175426
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal NAPSIN A antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAPSA aa 1-200.

View Alternative Names

NAP1, NAPA, NAPSA, Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [10C4B8] (AB175426)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [10C4B8] (AB175426)

Immunohistochemical analysis of paraffin-embedded liver cancer tissues labeling NAPSIN A with ab175426 at 1/200 dilution with DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [10C4B8] (AB175426)

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues labeling NAPSIN A with ab175426 at 1/200 dilution, with DAB staining.

Supplier Data

Western blot - Anti-NAPSIN A antibody [10C4B8] (AB175426)

All lanes:

Western blot - Anti-NAPSIN A antibody [10C4B8] (ab175426) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

NAPSIN A (AA: 20-158)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 119 kDa,142 kDa,28 kDa,33 kDa,45 kDa,46 kDa,95 kDa

false

Supplier Data

Western blot - Anti-NAPSIN A antibody [10C4B8] (AB175426)

All lanes:

Western blot - Anti-NAPSIN A antibody [10C4B8] (ab175426) at 1/500 dilution

All lanes:

NAPSIN recombinant protein

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-NAPSIN A antibody [10C4B8] (AB175426)

All lanes:

Western blot - Anti-NAPSIN A antibody [10C4B8] (ab175426) at 1/500 dilution

All lanes:

Rat liver tissue lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10C4B8

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NAPSA aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O96009

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Napsin A also known as napsin or napsine is an aspartic proteinase with a molecular weight of approximately 38 kDa. This enzyme is primarily expressed in the lung tissue renal tubules and in some cases thymus and spleen. Napsin A plays a significant role in protein catabolism by degrading specific peptide bonds facilitating protein turnover and maintenance of cellular homeostasis. It is often useful in identifying cell types being napsin A positive in certain cells and napsin A negative in others.

Biological function summary

Napsin A functions in the maturation of pulmonary surfactant proteins which are essential for normal lung function. Napsin A cleaves surfactant protein B an important component of the surfactant system in alveolar air spaces allowing proper lung mechanics. While napsin A operates in concert with other enzymes it does not form part of a larger complex. Its role impacts cellular processes related to respiratory physiology and contributes to the regulation of protein breakdown within the cell.

Pathways

Napsin A is involved in respiratory and proteolytic pathways influencing lung physiology and systemic protein metabolism. One important pathway is the surfactant metabolism pathway which ensures adequate surfactant levels for maintaining lung function. Napsin A is related to surfactant proteins like surfactant protein B through this pathway facilitating their activation and processing.

Napsin A serves as a biomarker for certain lung adenocarcinomas where napsin A positive lung tumors often indicate differential diagnosis between primary lung cancer and metastases from other sites. Napsin A's expression is linked to the presence of adenocarcinoma a specific type of lung cancer. In renal disorders napsin A expression helps distinguish renal cell carcinoma from other renal masses where its expression pattern relates to other proteins like thyroid transcription factor-1 in diagnosing specific cancer types.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May be involved in processing of pneumocyte surfactant precursors.

See full target information NAPSA

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 169:750-765.e17 PubMed28475900

2017

Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Yonit Lavin,Soma Kobayashi,Andrew Leader,El-Ad David Amir,Naama Elefant,Camille Bigenwald,Romain Remark,Robert Sweeney,Christian D Becker,Jacob H Levine,Klaus Meinhof,Andrew Chow,Seunghee Kim-Shulze,Andrea Wolf,Chiara Medaglia,Hanjie Li,Julie A Rytlewski,Ryan O Emerson,Alexander Solovyov,Benjamin D Greenbaum,Catherine Sanders,Marissa Vignali,Mary Beth Beasley,Raja Flores,Sacha Gnjatic,Dana Pe'er,Adeeb Rahman,Ido Amit,Miriam Merad

View all publications

style

left-column

style

left-column

style

right-column