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AB166619

Anti-NAPSIN A antibody [EPR10713(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal NAPSIN A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NAP1, NAPA, NAPSA, Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NAPSIN A antibody [EPR10713(B)] (AB166619)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NAPSIN A antibody [EPR10713(B)] (AB166619)

Immunofluorescence analysis of A549 cells labeling NAPSIN A with ab166619 at 1/250 dilution.

Western blot - Anti-NAPSIN A antibody [EPR10713(B)] (AB166619)
  • WB

Unknown

Western blot - Anti-NAPSIN A antibody [EPR10713(B)] (AB166619)

All lanes:

Western blot - Anti-NAPSIN A antibody [EPR10713(B)] (ab166619) at 1/10000 dilution

All lanes:

Human lung adenocarcinoma lysate at 10 µg

Predicted band size: 45 kDa

false

  • Carrier free

    Anti-NAPSIN A antibody [EPR10713(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10713(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Napsin A also known as napsin or napsine is an aspartic proteinase with a molecular weight of approximately 38 kDa. This enzyme is primarily expressed in the lung tissue renal tubules and in some cases thymus and spleen. Napsin A plays a significant role in protein catabolism by degrading specific peptide bonds facilitating protein turnover and maintenance of cellular homeostasis. It is often useful in identifying cell types being napsin A positive in certain cells and napsin A negative in others.
Biological function summary

Napsin A functions in the maturation of pulmonary surfactant proteins which are essential for normal lung function. Napsin A cleaves surfactant protein B an important component of the surfactant system in alveolar air spaces allowing proper lung mechanics. While napsin A operates in concert with other enzymes it does not form part of a larger complex. Its role impacts cellular processes related to respiratory physiology and contributes to the regulation of protein breakdown within the cell.

Pathways

Napsin A is involved in respiratory and proteolytic pathways influencing lung physiology and systemic protein metabolism. One important pathway is the surfactant metabolism pathway which ensures adequate surfactant levels for maintaining lung function. Napsin A is related to surfactant proteins like surfactant protein B through this pathway facilitating their activation and processing.

Napsin A serves as a biomarker for certain lung adenocarcinomas where napsin A positive lung tumors often indicate differential diagnosis between primary lung cancer and metastases from other sites. Napsin A's expression is linked to the presence of adenocarcinoma a specific type of lung cancer. In renal disorders napsin A expression helps distinguish renal cell carcinoma from other renal masses where its expression pattern relates to other proteins like thyroid transcription factor-1 in diagnosing specific cancer types.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in processing of pneumocyte surfactant precursors.
See full target information NAPSA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 44:3386-3406 PubMed40696167

2025

Glutaminase as a metabolic target of choice to counter acquired resistance to Palbociclib by colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Míriam Tarrado-Castellarnau,Carles Foguet,Josep Tarragó-Celada,Marc Palobart,Claudia Hernández-Carro,Jordi Perarnau,Erika Zodda,Ibrahim H Polat,Silvia Marin,Alejandro Suarez-Bonnet,Juan José Lozano,Mariia Yuneva,Timothy M Thomson,Marta Cascante
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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