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AB133249

Anti-NAPSIN A antibody [EPR6252]

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(7 Publications)

Rabbit Recombinant Monoclonal NAPSIN A antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

NAP1, NAPA, NAPSA, Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)

ab133249 showing positive staining in Normal lung tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)

ab133249 showing positive staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling NAPSIN A with ab133249 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-NAPSIN A antibody [EPR6252] (AB133249)
  • WB

Unknown

Western blot - Anti-NAPSIN A antibody [EPR6252] (AB133249)

All lanes:

Western blot - Anti-NAPSIN A antibody [EPR6252] (ab133249) at 1/10000 dilution

All lanes:

Human lung adenocarcinoma tissue lysate at 10 µg

Predicted band size: 123 kDa,29 kDa,45 kDa

Observed band size: 30 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] (AB133249)

ab133249 showing negative staining in Lung squamous carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Carrier free

    Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6252

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Napsin A also known as napsin or napsine is an aspartic proteinase with a molecular weight of approximately 38 kDa. This enzyme is primarily expressed in the lung tissue renal tubules and in some cases thymus and spleen. Napsin A plays a significant role in protein catabolism by degrading specific peptide bonds facilitating protein turnover and maintenance of cellular homeostasis. It is often useful in identifying cell types being napsin A positive in certain cells and napsin A negative in others.
Biological function summary

Napsin A functions in the maturation of pulmonary surfactant proteins which are essential for normal lung function. Napsin A cleaves surfactant protein B an important component of the surfactant system in alveolar air spaces allowing proper lung mechanics. While napsin A operates in concert with other enzymes it does not form part of a larger complex. Its role impacts cellular processes related to respiratory physiology and contributes to the regulation of protein breakdown within the cell.

Pathways

Napsin A is involved in respiratory and proteolytic pathways influencing lung physiology and systemic protein metabolism. One important pathway is the surfactant metabolism pathway which ensures adequate surfactant levels for maintaining lung function. Napsin A is related to surfactant proteins like surfactant protein B through this pathway facilitating their activation and processing.

Napsin A serves as a biomarker for certain lung adenocarcinomas where napsin A positive lung tumors often indicate differential diagnosis between primary lung cancer and metastases from other sites. Napsin A's expression is linked to the presence of adenocarcinoma a specific type of lung cancer. In renal disorders napsin A expression helps distinguish renal cell carcinoma from other renal masses where its expression pattern relates to other proteins like thyroid transcription factor-1 in diagnosing specific cancer types.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in processing of pneumocyte surfactant precursors.
See full target information NAPSA
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:282 PubMed41057919

2025

Long-term patient-derived ovarian cancer organoids closely recapitulate tumor of origin and clinical response.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Thorel,Enora Dolivet,Pierre-Marie Morice,Romane Florent,Jordane Divoux,Marion Perréard,Lucie Lecouflet,Guillaume Desmartin,Chloé Marde Alagama,Florence Giffard,Alexandra Leconte,Justine Lequesne,Bénédicte Clarisse,Mélanie Briand,Alimatou Traoré,Céline Villenet,Jean-Pascal Meneboo,Guillaume Babin,Léopold Gaichies,Sandrine Martin-Françoise,Jean-François Le Brun,Roman Rouzier,Emilie Brotin,Christophe Denoyelle,Nicolas Vigneron,Raphaël Leman,Dominique Vaur,Laurent Castera,Cécile Blanc-Fournier,Nicolas Elie,Benoit Plancoulaine,Florence Joly,Matthieu Meryet-Figuière,Martin Figeac,Louis-Bastien Weiswald,Laurent Poulain

Journal of experimental & clinical cancer research : CR 42:260 PubMed37803448

2023

Comparative analysis of response to treatments and molecular features of tumor-derived organoids versus cell lines and PDX derived from the same ovarian clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Thorel,Pierre-Marie Morice,Hippolyte Paysant,Romane Florent,Guillaume Babin,Cécilia Thomine,Marion Perréard,Edwige Abeilard,Florence Giffard,Emilie Brotin,Christophe Denoyelle,Céline Villenet,Shéhérazade Sebda,Mélanie Briand,Florence Joly,Enora Dolivet,Didier Goux,Cécile Blanc-Fournier,Corinne Jeanne,Marie Villedieu,Matthieu Meryet-Figuiere,Martin Figeac,Laurent Poulain,Louis-Bastien Weiswald

Oncology letters 16:2533-2538 PubMed30008942

2018

Overexpression of Napsin A resensitizes drug-resistant lung cancer A549 cells to gefitinib by inhibiting EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Linshui Zhou,Xin Lv,Junchao Yang,Yuanhong Zhu,Zhen Wang,Tingzhen Xu

Molecular medicine reports 18:1247-1252 PubMed29845258

2018

Napsin A is negatively associated with EMT‑mediated EGFR‑TKI resistance in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Linshui Zhou,Xin Lv,Junchao Yang,Yuanhong Zhu,Zhen Wang,Tingzhen Xu

Anticancer research 38:179-186 PubMed29277771

2017

Effects of siRNA Silencing of TUG1 and LCAL6 Long Non-coding RNAs on Patient-derived Xenograft of Non-small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Fang,Hairong Huang,Xiaoyou Li,Jing Liao,Zhijian Yang,Robert M Hoffman,X I Cheng,Lei Liang,Wenjuan Hu,Shifeng Yun

Turkish journal of medical sciences 46:1854-1861 PubMed28081338

2016

New immunohistochemical markers in the differential diagnosisof nonsmall cell lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ferda Bir,Duygu Çeliker,Binnaz Fatma Evyapan,Arzu Yaren,Tamer Edirne

Clinical cancer research : an official journal of the American Association for Cancer Research 21:1172-82 PubMed25549722

2015

Patient-derived xenografts from non-small cell lung cancer brain metastases are valuable translational platforms for the development of personalized targeted therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Won Lee,Jung-Il Lee,Se Jeong Lee,Hyun Jung Cho,Hye Jin Song,Da Eun Jeong,Yun Jee Seo,Sang Shin,Je-Gun Joung,Yong-Jun Kwon,Yoon-La Choi,Woong-Yang Park,Hyun Moo Lee,Ho Jun Seol,Young Mog Shim,Kyeung Min Joo,Do-Hyun Nam
View all publications
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