AB248340

Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NAPSIN A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NAP1, NAPA, NAPSA, Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

This data was developed using ab129189, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with unpurified ab129189 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

This data was developed using ab129189, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with purified ab129189 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Lab

Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

This data was developed using ab129189, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NAPSIN A antibody [EPR6257] (<a href='/en-us/products/primary-antibodies/napsin-a-antibody-epr6257-ab129189'>ab129189</a>) at 1/5000 dilution

All lanes:

Human lung adenocarcinoma tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 50 kDa

false

Unknown

Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

This data was developed using ab129189, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NAPSIN A antibody [EPR6257] (<a href='/en-us/products/primary-antibodies/napsin-a-antibody-epr6257-ab129189'>ab129189</a>) at 1/1000 dilution

All lanes:

Human lung adenocarcinoma lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 45 kDa

false

OI-RD Scanning - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-NAPSIN A antibody [EPR6257]
660 APC

APC Anti-NAPSIN A antibody [EPR6257]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NAPSIN A antibody [EPR6257]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-NAPSIN A antibody [EPR6257]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NAPSIN A antibody [EPR6257]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-NAPSIN A antibody [EPR6257]
578 PE

PE Anti-NAPSIN A antibody [EPR6257]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-NAPSIN A antibody [EPR6257]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6257

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab248340 is the carrier-free version of ab129189.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Napsin A also known as napsin or napsine is an aspartic proteinase with a molecular weight of approximately 38 kDa. This enzyme is primarily expressed in the lung tissue renal tubules and in some cases thymus and spleen. Napsin A plays a significant role in protein catabolism by degrading specific peptide bonds facilitating protein turnover and maintenance of cellular homeostasis. It is often useful in identifying cell types being napsin A positive in certain cells and napsin A negative in others.

Biological function summary

Napsin A functions in the maturation of pulmonary surfactant proteins which are essential for normal lung function. Napsin A cleaves surfactant protein B an important component of the surfactant system in alveolar air spaces allowing proper lung mechanics. While napsin A operates in concert with other enzymes it does not form part of a larger complex. Its role impacts cellular processes related to respiratory physiology and contributes to the regulation of protein breakdown within the cell.

Pathways

Napsin A is involved in respiratory and proteolytic pathways influencing lung physiology and systemic protein metabolism. One important pathway is the surfactant metabolism pathway which ensures adequate surfactant levels for maintaining lung function. Napsin A is related to surfactant proteins like surfactant protein B through this pathway facilitating their activation and processing.

Napsin A serves as a biomarker for certain lung adenocarcinomas where napsin A positive lung tumors often indicate differential diagnosis between primary lung cancer and metastases from other sites. Napsin A's expression is linked to the presence of adenocarcinoma a specific type of lung cancer. In renal disorders napsin A expression helps distinguish renal cell carcinoma from other renal masses where its expression pattern relates to other proteins like thyroid transcription factor-1 in diagnosing specific cancer types.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in processing of pneumocyte surfactant precursors.

See full target information NAPSA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of medicine and surgery (2012) 86:2644-2650 PubMed38694326

2024

S100A6 could not promote the differentiation of Calu-6 lung cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Ting Wang,Dan Dang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

OI-RD Scanning - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (AB248340)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise