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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse Monoclonal NAPSIN A antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NAPSA.
IgG1
Mouse
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
Flow Cyt | IHC-P | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes 30 minutes at room temperature in an ABC method.Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining |
May be involved in processing of pneumocyte surfactant precursors.
Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4, NAPA, NAP1, NAPSA
Mouse Monoclonal NAPSIN A antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NAPSA.
Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4, NAPA, NAP1, NAPSA
IgG1
Mouse
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
KCG1.1
IgG fraction
Napsin A reactivity was determined immunohistochemically on formalin-fixed paraffin-embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). Napsin A was positive in 10/12 pulmonary adenocarcinomas. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81%), primary lesions were positive.
Blue Ice
1-2 weeks
+4°C
-20°C
Avoid freeze / thaw cycle
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Napsin A functions in the maturation of pulmonary surfactant proteins which are essential for normal lung function. Napsin A cleaves surfactant protein B an important component of the surfactant system in alveolar air spaces allowing proper lung mechanics. While napsin A operates in concert with other enzymes it does not form part of a larger complex. Its role impacts cellular processes related to respiratory physiology and contributes to the regulation of protein breakdown within the cell.
Napsin A also known as napsin or napsine is an aspartic proteinase with a molecular weight of approximately 38 kDa. This enzyme is primarily expressed in the lung tissue renal tubules and in some cases thymus and spleen. Napsin A plays a significant role in protein catabolism by degrading specific peptide bonds facilitating protein turnover and maintenance of cellular homeostasis. It is often useful in identifying cell types being napsin A positive in certain cells and napsin A negative in others.
Napsin A is involved in respiratory and proteolytic pathways influencing lung physiology and systemic protein metabolism. One important pathway is the surfactant metabolism pathway which ensures adequate surfactant levels for maintaining lung function. Napsin A is related to surfactant proteins like surfactant protein B through this pathway facilitating their activation and processing.
Napsin A serves as a biomarker for certain lung adenocarcinomas where napsin A positive lung tumors often indicate differential diagnosis between primary lung cancer and metastases from other sites. Napsin A's expression is linked to the presence of adenocarcinoma a specific type of lung cancer. In renal disorders napsin A expression helps distinguish renal cell carcinoma from other renal masses where its expression pattern relates to other proteins like thyroid transcription factor-1 in diagnosing specific cancer types.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Paraffin embedded mouse lung adenocarcinoma tissue stained for Napsin A using ab73021 in immunohistochemical analysis.
Overlay histogram showing A549 cells stained with ab73021 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73021, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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