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AB73021

Anti-NAPSIN A antibody [KCG1.1]

4

(1 Review)

|

(5 Publications)

Mouse Monoclonal NAPSIN A antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NAPSA.

View Alternative Names

NAP1, NAPA, NAPSA, Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4

2 Images
Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (AB73021)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (AB73021)

Overlay histogram showing A549 cells stained with ab73021 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73021, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [KCG1.1] (AB73021)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [KCG1.1] (AB73021)

Paraffin embedded mouse lung adenocarcinoma tissue stained for Napsin A using ab73021 in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KCG1.1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human NAPSA. The exact immunogen used to generate this antibody is proprietary information.

O96009

Specificity

<p>Napsin A reactivity was determined immunohistochemically on formalin-fixed paraffin-embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). Napsin A was positive in 10/12 pulmonary adenocarcinomas. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81%), primary lesions were positive.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>30 minutes at room temperature in an ABC method.Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining</p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Napsin A also known as napsin or napsine is an aspartic proteinase with a molecular weight of approximately 38 kDa. This enzyme is primarily expressed in the lung tissue renal tubules and in some cases thymus and spleen. Napsin A plays a significant role in protein catabolism by degrading specific peptide bonds facilitating protein turnover and maintenance of cellular homeostasis. It is often useful in identifying cell types being napsin A positive in certain cells and napsin A negative in others.
Biological function summary

Napsin A functions in the maturation of pulmonary surfactant proteins which are essential for normal lung function. Napsin A cleaves surfactant protein B an important component of the surfactant system in alveolar air spaces allowing proper lung mechanics. While napsin A operates in concert with other enzymes it does not form part of a larger complex. Its role impacts cellular processes related to respiratory physiology and contributes to the regulation of protein breakdown within the cell.

Pathways

Napsin A is involved in respiratory and proteolytic pathways influencing lung physiology and systemic protein metabolism. One important pathway is the surfactant metabolism pathway which ensures adequate surfactant levels for maintaining lung function. Napsin A is related to surfactant proteins like surfactant protein B through this pathway facilitating their activation and processing.

Napsin A serves as a biomarker for certain lung adenocarcinomas where napsin A positive lung tumors often indicate differential diagnosis between primary lung cancer and metastases from other sites. Napsin A's expression is linked to the presence of adenocarcinoma a specific type of lung cancer. In renal disorders napsin A expression helps distinguish renal cell carcinoma from other renal masses where its expression pattern relates to other proteins like thyroid transcription factor-1 in diagnosing specific cancer types.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in processing of pneumocyte surfactant precursors.
See full target information NAPSA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 46:141-148 PubMed32319530

2020

TGF‑β1 induces CREB1‑mediated miR‑1290 upregulation to antagonize lung fibrosis via Napsin A.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhong Guan,Yudi Wu,Qiudi Zhang,Jun Zhou

Diagnostic pathology 14:96 PubMed31455365

2019

ALK rearrangement: a high-frequency alteration in ovarian metastasis from lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Bi,Qianming Bai,Xiaoli Zhu,Xiaoyu Tu,Xu Cai,Wenhua Jiang,Xiaoli Xu,Shaoxian Tang,Huijuan Ge,Bin Chang,Yufan Cheng,Hualei Gan,Xiaoyan Zhou,Wentao Yang

Cellular physiology and biochemistry : internation 44:2138-2146 PubMed29241190

2017

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangmin Zhao,Le Li,Qian Wang,Hongxiu Han,Qing Zhan,Ming Xu

Oncology letters 14:6645-6649 PubMed29151911

2017

Multiple pulmonary metastases with halo-sign from malignant mixed Müllerian tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Wei Tian,Wei-Bing Yang,Meng-Jie Yang,Jing-Yuan Liu,Jian-Chu Zhang,Xiao-Nan Tao,Qiong Zhou

Diagnostic cytopathology 44:299-304 PubMed26799356

2016

Utility of TTF-1 and Napsin-A in the work-up of malignant effusions.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed El Hag,Lindsay Schmidt,Michael Roh,Claire W Michael
View all publications
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