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AB154237

Anti-Nardilysin antibody

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(1 Publication)

Rabbit Polyclonal Nardilysin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NRDC aa 500-800.

View Alternative Names

NRD1, NRDC, Nardilysin, N-arginine dibasic convertase, Nardilysin convertase, NRD convertase, NRD-C

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nardilysin antibody (AB154237)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nardilysin antibody (AB154237)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling Nardilysin with ab154237 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Western blot - Anti-Nardilysin antibody (AB154237)
  • WB

Unknown

Western blot - Anti-Nardilysin antibody (AB154237)

5% SDS PAGE

All lanes:

Western blot - Anti-Nardilysin antibody (ab154237) at 1/2000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 132 kDa

false

Western blot - Anti-Nardilysin antibody (AB154237)
  • WB

Unknown

Western blot - Anti-Nardilysin antibody (AB154237)

5% SDS PAGE

All lanes:

Western blot - Anti-Nardilysin antibody (ab154237) at 1/1000 dilution

All lanes:

Mouse kidney whole cell lysate at 50 µg

Predicted band size: 132 kDa,134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NRDC aa 500-800. The exact immunogen used to generate this antibody is proprietary information.

O43847

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Complementary Products

Cell Lines & Lysates

AB263283

Human NRD1 (Nardilysin) knockout HEK-293T cell lysate

Sanger Sequencing - Human NRD1 (Nardilysin) knockout HEK-293T cell lysate (AB263283)

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Primary Antibodies

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Primary Antibodies

AB233443

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Cell Lines & Lysates

AB266385

Human NRD1 (Nardilysin) knockout HEK-293T cell line

Sanger Sequencing - Human NRD1 (Nardilysin) knockout HEK-293T cell line (AB266385)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nardilysin also referred to as NRD1 is a metalloendopeptidase that plays a role in protein processing. It possesses a molecular weight of approximately 124 kDa. Nardilysin is part of the M16 family of peptidases and is located predominantly in the cytoplasm but can also be found in the cell nucleus. The protein exhibits expression in a wide range of tissues indicating its widespread functional importance.
Biological function summary

Nardilysin impacts several cellular processes due to its role in cleaving specific peptides. It serves as a modulator of protein-protein interactions and is involved in the regulation of cell proliferation and differentiation. Nardilysin participates in the processing of various bioactive peptides and proteins and can function as part of a protein complex in some contexts. Its regulatory activities suggest its important role in maintaining cellular function and integrity.

Pathways

Nardilysin is involved in the promotion of the fibroblast growth factor (FGF) pathway and the insulin-like growth factor (IGF) signaling pathway. In these pathways it interacts with other proteins such as FGF receptors and IGF-binding proteins influencing cellular growth signaling and metabolic regulation. Nardilysin's involvement highlights its capacity to modulate key biological processes which are essential for normal cellular responses.

Nardilysin is linked with neurological disorders such as Alzheimer's disease where it is known to interact with amyloid precursor protein (APP) which plays a significant role in the disease's pathogenesis. Moreover its dysregulation has associations with metabolic disorders like obesity impacting proteins such as leptin and affecting energy balance. These associations emphasize the clinical significance of nardilysin as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves peptide substrates on the N-terminus of arginine residues in dibasic pairs. Is a critical activator of BACE1- and ADAM17-mediated pro-neuregulin ectodomain shedding, involved in the positive regulation of axonal maturation and myelination. Required for proper functioning of 2-oxoglutarate dehydrogenase (OGDH) (By similarity).
See full target information NRDC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:960 PubMed28424461

2017

A lack of coordination between sister-chromatids segregation and cytokinesis in the oocytes of B6.Y (XY) sex-reversed female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Qiao Zhu,Seang Lin Tan,Teruko Taketo
View all publications
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Product promise

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