Rabbit Polyclonal NARG1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NAA15.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NAA15. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXJ9
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Auxillary subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase (NAT) activity (PubMed:15496142, PubMed:20154145, PubMed:29754825, PubMed:32042062). The NAT activity may be important for vascular, hematopoietic and neuronal growth and development (PubMed:15496142). Required to control retinal neovascularization in adult ocular endothelial cells (PubMed:11687548). In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter (PubMed:12145306).
Alternative names
GA19, NARG1, NATH, TBDN100, NAA15, Gastric cancer antigen Ga19, N-terminal acetyltransferase, NMDA receptor-regulated protein 1, Protein tubedown-1, Tbdn100
Recommended products
Rabbit Polyclonal NARG1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NAA15.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NAA15. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXJ9
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NARG1 also known as NARGG or NMDA receptor-regulated gene protein 1 is a protein with a molecular mass of approximately 152 kDa. NARG1 directly participates in protein acetylation by transferring acetyl groups to lysine residues an important post-translational modification affecting protein function and interactions. This protein is expressed across various tissues with notable presence in the brain heart and testis indicating its diverse functional roles within different physiological contexts.
- Biological function summary
The acetyltransferase activity of NARG1 significantly impacts gene regulation and cellular homeostasis. It forms part of the NatA complex where it functions together with other components such as NAA10 and NAA15. This complex plays a role in co-translational N-terminal acetylation influencing protein stability and function. NARG1's activity particulary affects processes like cell cycle progression apoptosis and cellular differentiation reflecting its importance in maintaining cellular balance.
- Pathways
NARG1 activity links prominently to the signaling pathway of mRNA translation which is essential for protein synthesis and cellular growth. Additionally it participates in the regulation of apoptosis pathways modulating cell survival in response to various stress signals. Proteins like p53 and BCL-2 show interactions with these pathways revealing how NARG1 may influence key cellular outcomes and decision points regarding survival and death.
- Associated diseases and disorders
Studies have connected NARG1 to neural development and certain neurodegenerative conditions. Aberrations in NARG1 expression or function may contribute to disorders like Alzheimer’s Disease potentially affecting neuronal survival and cognitive function. The protein's role is also noteworthy in the context of cardiac hypertrophy where its regulatory capacity on cellular growth pathways can impact heart muscle function. Related proteins affected by these conditions may include components like tau in neurodegeneration and myosin heavy chain in cardiac pathophysiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NARG1 antibody (ab65107)
All lanes: Western blot - Anti-NARG1 antibody (ab65107) at 1/500 dilution
Lane 1: extracts from HUVEC cells at 5 µg
Lane 2: extracts from HUVEC cells at 5 µg with immunising peptide
Predicted band size: 101 kDa
Observed band size: 101 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NARG1 antibody (ab65107)
ICC/IF image of ab65107 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65107, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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