AB65107

Anti-NARG1 antibody

Name: Anti-NARG1 antibody (ab65107) | Abcam
Brand: Abcam Limited
SKU: AB65107
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NARG1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NAA15.

View Alternative Names

GA19, NARG1, NATH, TBDN100, NAA15, Gastric cancer antigen Ga19, N-terminal acetyltransferase, NMDA receptor-regulated protein 1, Protein tubedown-1, Tbdn100

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NARG1 antibody (AB65107)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NARG1 antibody (AB65107)

ICC/IF image of ab65107 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65107, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-NARG1 antibody (AB65107)

All lanes:

Western blot - Anti-NARG1 antibody (ab65107) at 1/500 dilution

Lane 1:

extracts from HUVEC cells at 5 µg

Lane 2:

extracts from HUVEC cells at 5 µg with immunising peptide

Predicted band size: 101 kDa

Observed band size: 101 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NAA15. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NARG1 also known as NARGG or NMDA receptor-regulated gene protein 1 is a protein with a molecular mass of approximately 152 kDa. NARG1 directly participates in protein acetylation by transferring acetyl groups to lysine residues an important post-translational modification affecting protein function and interactions. This protein is expressed across various tissues with notable presence in the brain heart and testis indicating its diverse functional roles within different physiological contexts.

Biological function summary

The acetyltransferase activity of NARG1 significantly impacts gene regulation and cellular homeostasis. It forms part of the NatA complex where it functions together with other components such as NAA10 and NAA15. This complex plays a role in co-translational N-terminal acetylation influencing protein stability and function. NARG1's activity particulary affects processes like cell cycle progression apoptosis and cellular differentiation reflecting its importance in maintaining cellular balance.

Pathways

NARG1 activity links prominently to the signaling pathway of mRNA translation which is essential for protein synthesis and cellular growth. Additionally it participates in the regulation of apoptosis pathways modulating cell survival in response to various stress signals. Proteins like p53 and BCL-2 show interactions with these pathways revealing how NARG1 may influence key cellular outcomes and decision points regarding survival and death.

Studies have connected NARG1 to neural development and certain neurodegenerative conditions. Aberrations in NARG1 expression or function may contribute to disorders like Alzheimer’s Disease potentially affecting neuronal survival and cognitive function. The protein's role is also noteworthy in the context of cardiac hypertrophy where its regulatory capacity on cellular growth pathways can impact heart muscle function. Related proteins affected by these conditions may include components like tau in neurodegeneration and myosin heavy chain in cardiac pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Auxillary subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase (NAT) activity (PubMed : 15496142, PubMed : 20154145, PubMed : 29754825, PubMed : 32042062). The NAT activity may be important for vascular, hematopoietic and neuronal growth and development (PubMed : 15496142). Required to control retinal neovascularization in adult ocular endothelial cells (PubMed : 11687548). In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter (PubMed : 12145306).

See full target information NAA15

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of clinical and translational neurology 8:558-570 PubMed33465303

2021

HCMV infection and IFITM3 rs12252 are associated with Rasmussen's encephalitis disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Song Wang,Qiao-Li Luo,Yu-Guang Guan,Dong-Ying Fan,Guo-Ming Luan,An Jing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NARG1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NARG1 antibody (AB65107)

Western blot - Anti-NARG1 antibody (AB65107)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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