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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Recombinant Monoclonal NARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | Flow Cyt | WB | ICC/IF | |
---|---|---|---|---|
Human | Tested | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed:9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells (PubMed:30171954, PubMed:12235211).
Asparaginyl-tRNA synthetase, Asparaginyl-tRNA synthetase 1, AsnRS, NARS1, NARS, NRS
Rabbit Recombinant Monoclonal NARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Asparaginyl-tRNA synthetase, Asparaginyl-tRNA synthetase 1, AsnRS, NARS1, NARS, NRS
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
EPR8221
Affinity purification Protein A
7.09 x 10-11 M
Blue Ice
-20°C
Stable for 12 months at -20°C
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
As an essential component of the protein synthesis machinery NARS functions to ensure the fidelity and efficiency of genetic translation. It does not form part of a larger complex but its activity is critical in maintaining cellular function by facilitating accurate amino acid incorporation into growing polypeptide chains. This precise activity is necessary for cellular viability and the prevention of erroneous protein synthesis which could lead to dysfunctional proteins and cellular stress.
NARS also known as asparaginyl-tRNA synthetase is an enzyme with a mass of about 65 kDa. This enzyme plays an important role in protein synthesis by catalyzing the attachment of asparagine to its corresponding tRNA. NARS is expressed in various tissues across the body with noticeable levels in the liver and brain. The enzyme operates as a homodimer and requires ATP for its catalytic activity which facilitates the formation of asparaginyl-tRNA ensuring proper translation of genetic information during protein synthesis.
NARS interacts with multiple biological pathways including the aminoacyl-tRNA biosynthesis pathway and the protein translation pathway. NARS’s role in aminoacyl-tRNA biosynthesis is important as it interacts with other aminoacyl-tRNA synthetases to maintain balanced protein synthesis. In the protein translation pathway it works alongside other elongation factors such as EF-Tu which ensures the correct delivery of aminoacyl-tRNA to the ribosome thereby orchestrating the translation process.
NARS's dysfunction links to neurodegenerative disorders and certain cancers. Mutations or aberrant expression of NARS may result in impaired protein synthesis contributing to neurological conditions such as Charcot-Marie-Tooth disease. Other proteins involved in these disorders include GARS another tRNA synthetase associated with similar hereditary neuropathies. Additionally increased expression of NARS has been noted in some cancerous tissues implicating its potential role in tumorigenesis and highlighting its relevance in cancer pathways.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab129162, at 1/50 dilution, staining NARS in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes: Western blot - Anti-NARS antibody [EPR8221] (AB129162) at 1/10000 dilution
Lane 1: Human fetal liver tissue lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
Lane 4: HT-1080 cell lysate at 10 µg
All lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 63 kDa
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com