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Rabbit Recombinant Monoclonal NARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed:9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells (PubMed:30171954, PubMed:12235211).

Alternative names

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Rabbit Recombinant Monoclonal NARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR8221

Purification technique

Affinity purification Protein A

Dissociation constant

7.09 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

As an essential component of the protein synthesis machinery NARS functions to ensure the fidelity and efficiency of genetic translation. It does not form part of a larger complex but its activity is critical in maintaining cellular function by facilitating accurate amino acid incorporation into growing polypeptide chains. This precise activity is necessary for cellular viability and the prevention of erroneous protein synthesis which could lead to dysfunctional proteins and cellular stress.

Mechanical summary

NARS also known as asparaginyl-tRNA synthetase is an enzyme with a mass of about 65 kDa. This enzyme plays an important role in protein synthesis by catalyzing the attachment of asparagine to its corresponding tRNA. NARS is expressed in various tissues across the body with noticeable levels in the liver and brain. The enzyme operates as a homodimer and requires ATP for its catalytic activity which facilitates the formation of asparaginyl-tRNA ensuring proper translation of genetic information during protein synthesis.

Pathway

NARS interacts with multiple biological pathways including the aminoacyl-tRNA biosynthesis pathway and the protein translation pathway. NARS’s role in aminoacyl-tRNA biosynthesis is important as it interacts with other aminoacyl-tRNA synthetases to maintain balanced protein synthesis. In the protein translation pathway it works alongside other elongation factors such as EF-Tu which ensures the correct delivery of aminoacyl-tRNA to the ribosome thereby orchestrating the translation process.

Disease

NARS's dysfunction links to neurodegenerative disorders and certain cancers. Mutations or aberrant expression of NARS may result in impaired protein synthesis contributing to neurological conditions such as Charcot-Marie-Tooth disease. Other proteins involved in these disorders include GARS another tRNA synthetase associated with similar hereditary neuropathies. Additionally increased expression of NARS has been noted in some cancerous tissues implicating its potential role in tumorigenesis and highlighting its relevance in cancer pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NARS antibody [EPR8221] (ab129162), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NARS antibody [EPR8221] (ab129162)

    ab129162, at 1/50 dilution, staining NARS in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-NARS antibody [EPR8221] (ab129162), expandable thumbnail

    Western blot - Anti-NARS antibody [EPR8221] (ab129162)

    All lanes: Western blot - Anti-NARS antibody [EPR8221] (AB129162) at 1/10000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Lane 4: HT-1080 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 63 kDa

  • OI-RD Scanning - Anti-NARS antibody [EPR8221] (ab129162), expandable thumbnail

    OI-RD Scanning - Anti-NARS antibody [EPR8221] (ab129162)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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