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AB69470

Anti-NARS2 antibody

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(1 Publication)

Mouse Polyclonal NARS2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NARS2.

View Alternative Names

Asparaginyl-tRNA synthetase, AsnRS, NARS2

2 Images
Western blot - Anti-NARS2 antibody (AB69470)
  • WB

Unknown

Western blot - Anti-NARS2 antibody (AB69470)

All lanes:

Western blot - Anti-NARS2 antibody (ab69470) at 1/500 dilution

Lane 1:

NARS2 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 54 kDa

Observed band size: 60 kDa

false

Western blot - Anti-NARS2 antibody (AB69470)
  • WB

Unknown

Western blot - Anti-NARS2 antibody (AB69470)

All lanes:

Western blot - Anti-NARS2 antibody (ab69470) at 1/500 dilution

All lanes:

human liver lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 54 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NARS2.

Q96I59

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NARS2 also known as mitochondrial asparaginyl-tRNA synthetase is a protein with a mass of approximately 51 kDa. It plays an important role in the process of protein synthesis within the mitochondria by attaching asparagine to its corresponding tRNA. NARS2 is expressed in tissues with high energy demands such as the heart muscle and brain. These areas highlight its importance in cellular energy production and metabolism.
Biological function summary

NARS2 functions in aminoacyl-tRNA biosynthesis. It is part of a group of enzymes called the aminoacyl-tRNA synthetases which are essential for the translation of the genetic code into proteins. This enzyme is involved in adding specific amino acids to their corresponding tRNAs therefore ensuring the accurate translation of mitochondrial mRNA into functional proteins. The precise function of NARS2 supports overall mitochondrial activity and energy production emphasizing its role in cellular health.

Pathways

NARS2 is critically involved in the mitochondrial translation pathway. This pathway is important for the proper functioning of the electron transport chain and ATP production. NARS2 works alongside other aminoacyl-tRNA synthetases and mitochondrial translation initiation factors to facilitate protein synthesis in mitochondria. Its activity directly impacts proteins such as the respiratory chain complex proteins which are necessary for efficient cellular respiration.

NARS2 mutations have been linked to mitochondrial diseases including mitochondrial encephalomyopathy and sensorineural hearing loss. These conditions result from impaired mitochondrial protein synthesis leading to reduced energy output. One related protein affected in these disorders is the complex I protein of the electron transport chain as its synthesis depends on proper mitochondrial function. NARS2's connection to these conditions emphasizes the importance of proper mitochondrial translation in maintaining cellular energy balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial aminoacyl-tRNA synthetase that catalyzes the specific attachment of the asparagine amino acid (aa) to the homologous transfer RNA (tRNA), further participating in protein synthesis (PubMed : 25385316). The reaction occurs in a two steps : asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (Probable).
See full target information NARS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biosensors 12: PubMed35323423

2022

A Novel Peptide as a Specific and Selective Probe for Detection.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Kim,Ju Hye Jang,In Young Jung,Ju Hyun Cho
View all publications
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Product promise

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