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AB91447

Anti-NAS6 antibody

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(3 Publications)

Rabbit Polyclonal Gankyrin antibody. Suitable for IP, WB and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C NAS6.

View Alternative Names

YGR232W, G8564, NAS6, Probable 26S proteasome regulatory subunit p28, Proteasome non-ATPase subunit 6, Nas6p, Proteasome non ATPase subunit 6, Probable 26S proteasome regulatory subunit p28, ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6, Proteasome interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP)

1 Images
Western blot - Anti-NAS6 antibody (AB91447)
  • WB

Unknown

Western blot - Anti-NAS6 antibody (AB91447)

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Western blot - Anti-NAS6 antibody (ab91447) at 1/5000 dilution

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Crude extract of <i>S. cerevisiae</i>

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C NAS6.

P50086

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6 - 8.5 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NAS6 also known as p28 and gankyrin is a regulatory component with an approximate mass of 28 kDa. NAS6 has significant expression in the human liver as well as detectable levels in several other tissues such as the brain and heart. Mechanically NAS6 interacts with the proteasome aiding in the regulation of protein degradation. The interaction with the proteasome involves NAS6 binding to specific subunits and helping in substrate recognition.
Biological function summary

NAS6 plays an important role in cellular protein homeostasis by associating with the 26S proteasome complex. This association facilitates the targeting and degradation of ubiquitinated proteins which is critical for maintaining cellular function. Through its activity NAS6 contributes to the removal of damaged or misfolded proteins and regulation of the cell cycle. This regulation includes controlling the abundance of cyclins and other proteins that are pivotal in cell division processes.

Pathways

NAS6 integrates into the ubiquitin-proteasome pathway a highly conserved pathway responsible for protein turnover in the cell. The pathway involves proteins like ubiquitin which tags proteins for degradation and the 26S proteasome where NAS6 plays its regulatory role. NAS6 also interacts with other proteins such as MDM2 in the p53 pathway linking its function to cellular stress responses and apoptosis regulation.

NAS6 has implications in oncogenesis particularly in liver cancer and colorectal cancer. Overexpression of NAS6 links to uncontrolled cell proliferation mostly due to its effect on protein degradation pathways. Additionally NAS6 interacts with the retinoblastoma protein (Rb) influencing pathways associated with tumorigenesis. Alterations in NAS6 levels or function can disrupt normal cellular regulation highlighting its relevance in cancer research and therapy development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.
See full target information NAS6

Additional targets

NAS6
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 24:1077-1087 PubMed35739319

2022

Actin remodelling controls proteasome homeostasis upon stress.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas David Williams,Roberta Cacioppo,Alexander Agrotis,Ailsa Black,Houjiang Zhou,Adrien Rousseau

eLife 8: PubMed31778111

2019

Specific lid-base contacts in the 26s proteasome control the conformational switching required for substrate degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Eric R Greene,Ellen A Goodall,Andres H de la Peña,Mary E Matyskiela,Gabriel C Lander,Andreas Martin

Nature 536:184-9 PubMed27462806

2016

An evolutionarily conserved pathway controls proteasome homeostasis.

Applications

WB

Species

Unspecified reactive species

Adrien Rousseau,Anne Bertolotti
View all publications
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Product promise

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