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AB250395

Anti-NASP antibody [EPR12827] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NASP antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Nuclear autoantigenic sperm protein, NASP

4 Images
Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)

This data was developed using ab181169, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells using ab181169 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/40 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)

This data was developed using ab181169, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

Immunoprecipitation - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)
  • IP

Supplier Data

Immunoprecipitation - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)

This data was developed using ab181169, the same antibody clone in a different buffer formulation.

Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-NASP antibody [EPR12827] (<a href='/en-us/products/primary-antibodies/nasp-antibody-epr12827-ab181169'>ab181169</a>)

Predicted band size: 85 kDa

false

Western blot - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)
  • WB

Supplier Data

Western blot - Anti-NASP antibody [EPR12827] - BSA and Azide free (AB250395)

This data was developed using ab181169, the same antibody clone in a different buffer formulation.

160KDa : tNASP

62KDa : sNASP.

All lanes:

Western blot - Anti-NASP antibody [EPR12827] (<a href='/en-us/products/primary-antibodies/nasp-antibody-epr12827-ab181169'>ab181169</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Caco-2 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

NTERA2/D1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12827

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250395 is the carrier-free version of ab181169.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NASP also known as Nuclear Autoantigenic Sperm Protein functions as a histone chaperone. With a molecular mass of approximately 75 kDa NASP assists in the proper assembly and disassembly of nucleosome structures by binding to histones H1 and H2A-H2B. This protein mainly expresses in rapidly dividing tissues especially in the testes but also presents in the thymus and bone marrow. NASP maintains the supply of free histones aiding in DNA replication and transcription processes.
Biological function summary

NASP plays an essential role in chromatin organization and dynamics during the cell cycle. As part of the histone transport complex it stabilizes histones and prevents their aggregation. NASP facilitates proper chromatin assembly necessary for DNA packaging and regulation of gene expression. This activity ensures the accurate transmission of genetic information during cell division impacting processes essential for cell proliferation and function.

Pathways

NASP operates within the chromatin assembly and nucleosome remodeling pathways. It coordinates with other chaperones like NAP1 in chromatin assembly factor-1 (CAF-1) complex. These pathways are integral to DNA replication and repair directly linking NASP to cellular processes critical for maintaining genome integrity. The interaction with importin beta further indicates NASP's role in nuclear import pathways emphasizing its involvement in both nuclear organization and cellular regulation.

NASP associates with cancer and infertility. The aberrant expression of NASP is observed in various tumors implying its contribution to tumorigenesis and proliferation. Its association with other histone chaperones such as ASF1 highlights its potential role in oncogenic pathways. Furthermore alterations in NASP expression affect sperm development linking it to male infertility and emphasizing its necessity in reproductive biology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the histone chaperone network (PubMed : 22195965). Binds and stabilizes histone H3-H4 not bound to chromatin to maintain a soluble reservoir and modulate degradation by chaperone-mediated autophagy (PubMed : 22195965). Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.. Isoform 1. Stabilizes soluble histone H3-H4.. Isoform 2. Stabilizes soluble histone H3-H4.
See full target information NASP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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