Anti-NAT10 antibody [EPR18663] - BSA and Azide free
- BOND RX™ Validated
- RabMAb
- Recombinant
- What is this?
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(2 Publications)
Rabbit Recombinant Monoclonal NAT10 antibody. Carrier free. Suitable for WB, IP, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
View Alternative Names
ALP, KIAA1709, NAT10, RNA cytidine acetyltransferase, 18S rRNA cytosine acetyltransferase, N-acetyltransferase 10, N-acetyltransferase-like protein, hALP
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on Human colon tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab1942997 the same antibody clone in a different buffer formulation
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling NAT10 with ab194297 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Nuclear staining in human colon cancer.The section was incubated with ab194297 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
NAT10 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate with ab194297 at 1/80 dilution.
Lane 1 : HeLa cell lysate 10ug (Input).
Lane 2 : ab194297 IP in HeLa cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab194297 in HeLa cell lysate.
Western blot was performed from the immunoprecipitate using ab194297 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.
All lanes:
Immunoprecipitation - Anti-NAT10 antibody [EPR18663] (<a href='/en-us/products/primary-antibodies/nat10-antibody-epr18663-ab194297'>ab194297</a>)
Predicted band size: 115 kDa
false
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NAT10 (red) with purified ab194297 at dilution of 1/600. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab1942997, the same antibody clone in a different buffer formulation
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling NAT10 with ab194297 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Nuclear staining in rat colon.
The section was incubated with ab194297 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab1942997, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling NAT10 with ab194297 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Nuclear staining in mouse stomach.
The section was incubated with ab194297 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on rat colon tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on NIH/3T3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on mouse stomach tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (AB251186)
This data was developed using ab194297, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.
Exposure time : Lane 1 : 1 second; Lane 2-7 : 40 seconds
Negative : kidney (PMID : 37349316).
All lanes:
Western blot - Anti-NAT10 antibody [EPR18663] (<a href='/en-us/products/primary-antibodies/nat10-antibody-epr18663-ab194297'>ab194297</a>) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3:
Mouse spleen tissue lysate at 20 µg
Lane 4:
Mouse lung tissue lysate at 20 µg
Lane 5:
Rat spleen tissue lysate at 20 µg
Lane 6:
Rat lung tissue lysate at 20 µg
Lane 7:
Rat kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 115 kDa
false
Related conjugates and formulations (1)
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Anti-NAT10 antibody [EPR18663]
Reactivity data
Product details
ab251186 is the carrier-free version of ab194297.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This enzyme plays a significant role in cellular activities by modulating chromatin dynamics and influencing cell cycle progression through acetylation of histones and non-histone proteins. NAT10 participates in rRNA transcription enhancing ribosomal biogenesis and therefore supporting protein synthesis within the cell. It functions as part of larger chromatin-associated complexes indicating its involvement in broader cellular systems beyond individual enzymatic activity.
Pathways
The NAT10 protein operates within important molecular pathways such as the p53 signaling and DNA damage response pathways. It contributes toward cellular response mechanisms to damage and stress acting alongside other proteins like p53 and CREB-binding protein connecting regulation of cell cycle arrest and apoptosis with its enzymatic activity. NAT10 also modulates the acetylation dynamics in these pathways affecting cellular outcomes under stress conditions.
Product protocols
- Visit the General protocols
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Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
International journal of biological sciences 21:4027-4050 PubMed40612676
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of medical sciences 20:1079-1090 PubMed37484809
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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