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AB241487

Anti-NAT13 antibody

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(1 Publication)

Rabbit Polyclonal NAT13 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NAA50 aa 100 to C-terminus.

View Alternative Names

MAK3, NAT13, NAT5, NAA50, N-alpha-acetyltransferase 50, hNaa50p, N-acetyltransferase 13, N-acetyltransferase 5, N-acetyltransferase san homolog, N-epsilon-acetyltransferase 50, NatE catalytic subunit, hNAT5, hSAN

2 Images
Immunoprecipitation - Anti-NAT13 antibody (AB241487)
  • IP

Supplier Data

Immunoprecipitation - Anti-NAT13 antibody (AB241487)

NAT13 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241487 at 20 μL per reaction. Western blot was perfromed on the immunoprecipitate using ab241487 at 1/250 dilution.

Lane 1 : ab241487 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NAT13 antibody (ab241487)

Predicted band size: 19 kDa

false

Western blot - Anti-NAT13 antibody (AB241487)
  • WB

Supplier Data

Western blot - Anti-NAT13 antibody (AB241487)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-NAT13 antibody (ab241487) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 19 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NAA50 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9GZZ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to NAT13 immobilized on solid support
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The N-acetyltransferase 13 (NAT13) also known as GCN5-related N-acetyltransferase-like 3 is a protein involved in acetylation processes. It has a molecular mass of about 25 kDa. NAT13 is known for its specific role in the acetylation of polyamines important for cellular metabolism. This protein is expressed in various tissues with significant presence in the liver and kidney. The widespread expression suggests its importance in maintaining normal cellular function.
Biological function summary

NAT13 exerts its effects on cellular growth modulation and proliferation. NAT13 functions as a part of the enzyme complexes that regulate the acetylation process modifying substrates in the cell. This modification impacts protein function stability and interaction with other cellular components. NAT13's enzymatic activity influences cell cycle regulation and is critical for normal cell growth and division.

Pathways

NAT13 influences polyamine biosynthesis and metabolism which are necessary for cell replication and growth. One of the significant pathways involving NAT13 is the spermidine and spermine biosynthesis pathway which interacts with the polyamine oxidase enzyme. These interactions help to balance cellular growth signals and maintain cellular homeostasis. Another associated pathway is histone acetylation impacting gene expression regulation and being correlated with the GCN5 protein.

NAT13 has connections to colorectal cancer and hepatocellular carcinoma due to its role in cell cycle regulation and proliferation. Aberrant activity of NAT13 can disrupt normal acetylation patterns leading to uncontrolled cell growth associated with tumorigenesis. Additionally NAT13's dysregulation links with proteins such as c-Myc known for their part in oncogenic processes. Understanding NAT13's activity offers insights into the molecular mechanisms behind these cancers and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine (PubMed : 19744929, PubMed : 21900231, PubMed : 22311970, PubMed : 27484799). Has a broad substrate specificity : able to acetylate the initiator methionine of most peptides, except for those with a proline in second position (PubMed : 27484799). Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins (PubMed : 19744929). Autoacetylates in vivo (PubMed : 19744929). The relevance of N-epsilon-acetyltransferase activity is however unclear : able to acetylate H4 in vitro, but this result has not been confirmed in vivo (PubMed : 19744929). Component of N-alpha-acetyltransferase complexes containing NAA10 and NAA15, which has N-alpha-acetyltransferase activity (PubMed : 16507339, PubMed : 27484799, PubMed : 29754825, PubMed : 32042062). Does not influence the acetyltransferase activity of NAA10 (PubMed : 16507339, PubMed : 27484799). However, it negatively regulates the N-alpha-acetyltransferase activity of the N-terminal acetyltransferase A complex (also called the NatA complex) (PubMed : 32042062). The multiprotein complexes probably constitute the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides (PubMed : 16507339, PubMed : 27484799). Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin : may act by counteracting the function of NAA10 (PubMed : 17502424, PubMed : 27422821).
See full target information NAA50
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neurotherapeutics : the journal of the American So : PubMed31898284

2020

CCR1 Activation Promotes Neuroinflammation Through CCR1/TPR1/ERK1/2 Signaling Pathway After Intracerebral Hemorrhage in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yan,Gang Zuo,Prativa Sherchan,Lei Huang,Umut Ocak,Weilin Xu,Zachary D Travis,Wenna Wang,John H Zhang,Jiping Tang
View all publications
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