Rabbit Polyclonal NAT2/AT-2 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Nat2 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Nat2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P50298
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Expected | Tested |
| Rat | Tested | Tested |
| Hamster | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.20000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-20.00000 µg/mL | Notes - |
Species Rat | Dilution info 5.00000-20.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates, and participates in the detoxification of a plethora of hydrazine and arylamine drugs.
Alternative names
Aac2, Nat2, Arylamine N-acetyltransferase 2, Arylamide acetylase 2, N-acetyltransferase type 2, N-hydroxyarylamine O-acetyltransferase, NAT-2
Recommended products
Rabbit Polyclonal NAT2/AT-2 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Nat2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Nat2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P50298
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NAT2 also known as N-acetyltransferase 2 or AT-2 is an important enzyme in drug metabolism. It catalyzes the acetylation process which plays a role in converting substances such as drugs and environmental chemicals into less active forms. This enzymatic activity requires co-factor acetyl-CoA. NAT2 has a molecular weight of about 33 kDa and is expressed mostly in liver and intestines. Its genetic polymorphisms influence enzyme activity leading to rapid or slow acetylator phenotypes among individuals.
- Biological function summary
NAT2 participates in the metabolism of aromatic amines which includes the acetylation of drugs like isoniazid and sulfamethazine. NAT2 does not typically form complexes with other proteins but its activity affects the concentration of metabolites that can interact with other pathways or proteins. It regulates the bioavailability and potential toxicity of different chemical substances in the body by modifying their structure and reactivity impacting pharmacokinetics significantly.
- Pathways
NAT2 is deeply involved in xenobiotic metabolism and Phase II drug metabolism pathways. It contributes directly to the N-acetylation pathway critical for the biotransformation of pharmaceuticals. NAT2 works in tandem with Cytochrome P450 enzymes which usually perform initial oxidative modifications on compounds destined for NAT2 acetylation. This interaction highlights NAT2's role in creating metabolic balance by processing potential toxins and facilitating their removal or detoxification from the body.
- Associated diseases and disorders
NAT2 polymorphisms play a critical role in susceptibility to certain conditions such as bladder cancer and drug-induced lupus erythematosus. Slow acetylator phenotypes are associated with higher risk of bladder cancer due to inefficient detoxification of carcinogens. Furthermore NAT2 interplays with proteins like N-acetyltransferase 1 (NAT1) influencing the biological responses and effectiveness of therapeutic strategies. Understanding the role of NAT2 in these disorders is essential for developing personalized medicine approaches to treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Western blot - Anti-NAT2/AT-2 antibody (ab232822)
Western blot - Anti-NAT2/AT-2 antibody (ab232822)
All lanes: Western blot - Anti-NAT2/AT-2 antibody (ab232822) at 2 µg/mL
All lanes: Mouse liver tissue lysate.
Predicted band size: 34 kDa
Western blot - Anti-NAT2/AT-2 antibody (ab232822)
All lanes: Western blot - Anti-NAT2/AT-2 antibody (ab232822) at 2 µg/mL
All lanes: Recombinant rat NAT2/AT-2 protein.
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT2/AT-2 antibody (ab232822)
Formalin-fixed, paraffin-embedded rat heart tissue stained for NAT2/AT-2 using ab232822 at 20μg/mL in immunohistochemical analysis. DAB staining.
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