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AB194114

Anti-NAT2/AT-2 antibody [EPR15856]

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(4 Publications)

Rabbit Recombinant Monoclonal NAT2/AT-2 antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Cited in 4 publications.

View Alternative Names

AAC2, NAT2, Arylamine N-acetyltransferase 2, Arylamide acetylase 2, N-acetyltransferase type 2, N-hydroxyarylamine O-acetyltransferase, Polymorphic arylamine N-acetyltransferase, NAT-2, PNAT

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NAT2/AT-2 antibody [EPR15856] (AB194114)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NAT2/AT-2 antibody [EPR15856] (AB194114)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HepG2 cells labeling NAT2/AT-2 with ab194114 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (AB194114)
  • WB

Supplier Data

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (AB194114)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/1000 dilution

All lanes:

Human colon cancer lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (AB194114)
  • WB

Supplier Data

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (AB194114)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/5000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

  • Carrier free

    Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15856

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAT2 also known as N-acetyltransferase 2 or AT-2 is an important enzyme in drug metabolism. It catalyzes the acetylation process which plays a role in converting substances such as drugs and environmental chemicals into less active forms. This enzymatic activity requires co-factor acetyl-CoA. NAT2 has a molecular weight of about 33 kDa and is expressed mostly in liver and intestines. Its genetic polymorphisms influence enzyme activity leading to rapid or slow acetylator phenotypes among individuals.
Biological function summary

NAT2 participates in the metabolism of aromatic amines which includes the acetylation of drugs like isoniazid and sulfamethazine. NAT2 does not typically form complexes with other proteins but its activity affects the concentration of metabolites that can interact with other pathways or proteins. It regulates the bioavailability and potential toxicity of different chemical substances in the body by modifying their structure and reactivity impacting pharmacokinetics significantly.

Pathways

NAT2 is deeply involved in xenobiotic metabolism and Phase II drug metabolism pathways. It contributes directly to the N-acetylation pathway critical for the biotransformation of pharmaceuticals. NAT2 works in tandem with Cytochrome P450 enzymes which usually perform initial oxidative modifications on compounds destined for NAT2 acetylation. This interaction highlights NAT2's role in creating metabolic balance by processing potential toxins and facilitating their removal or detoxification from the body.

NAT2 polymorphisms play a critical role in susceptibility to certain conditions such as bladder cancer and drug-induced lupus erythematosus. Slow acetylator phenotypes are associated with higher risk of bladder cancer due to inefficient detoxification of carcinogens. Furthermore NAT2 interplays with proteins like N-acetyltransferase 1 (NAT1) influencing the biological responses and effectiveness of therapeutic strategies. Understanding the role of NAT2 in these disorders is essential for developing personalized medicine approaches to treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates (PubMed : 12222688, PubMed : 7915226). Participates in the detoxification of a plethora of hydrazine and arylamine drugs, and is able to bioactivate several known carcinogens.
See full target information NAT2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Toxicological sciences : an official journal of the Society of Toxicology 190:158-172 PubMed36156098

2022

Transcriptional Regulation of Human Arylamine N-Acetyltransferase 2 Gene by Glucose and Insulin in Liver Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung U Hong,Raúl A Salazar-González,Kennedy M Walls,David W Hein

Toxicology and applied pharmacology 442:115993 PubMed35353990

2022

Expression of arylamine N-acetyltransferase 2 activity in immortalized human bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

James T F Wise,Raúl A Salazar-González,Mariam R Habil,Mark A Doll,David W Hein

Scientific reports 10:7566 PubMed32372066

2020

Human arylamine N-acetyltransferase 2 genotype-dependent protein expression in cryopreserved human hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Raúl A Salazar-González,Mark A Doll,David W Hein

Journal of oncology 2019:3860426 PubMed31531019

2019

-Acetyltransferase 1 Knockout Elevates Acetyl Coenzyme A Levels and Reduces Anchorage-Independent Growth in Human Breast Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Marcus W Stepp,Raúl A Salazar-González,Kyung U Hong,Mark A Doll,David W Hein
View all publications
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Product promise

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