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AB251180

Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free

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Rabbit Recombinant Monoclonal NAT2/AT-2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat samples.

View Alternative Names

AAC2, NAT2, Arylamine N-acetyltransferase 2, Arylamide acetylase 2, N-acetyltransferase type 2, N-hydroxyarylamine O-acetyltransferase, Polymorphic arylamine N-acetyltransferase, NAT-2, PNAT

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (AB251180)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (AB251180)

This data was developed using ab194114, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling NAT2/AT-2 with ab194114 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (AB251180)
  • WB

Supplier Data

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (AB251180)

This data was developed using ab194114, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (<a href='/en-us/products/primary-antibodies/nat2-at-2-antibody-epr15856-ab194114'>ab194114</a>) at 1/1000 dilution

All lanes:

Human colon cancer lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (AB251180)
  • WB

Supplier Data

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (AB251180)

This data was developed using ab194114, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (<a href='/en-us/products/primary-antibodies/nat2-at-2-antibody-epr15856-ab194114'>ab194114</a>) at 1/5000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15856

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251180 is the carrier-free version of ab194114.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAT2 also known as N-acetyltransferase 2 or AT-2 is an important enzyme in drug metabolism. It catalyzes the acetylation process which plays a role in converting substances such as drugs and environmental chemicals into less active forms. This enzymatic activity requires co-factor acetyl-CoA. NAT2 has a molecular weight of about 33 kDa and is expressed mostly in liver and intestines. Its genetic polymorphisms influence enzyme activity leading to rapid or slow acetylator phenotypes among individuals.
Biological function summary

NAT2 participates in the metabolism of aromatic amines which includes the acetylation of drugs like isoniazid and sulfamethazine. NAT2 does not typically form complexes with other proteins but its activity affects the concentration of metabolites that can interact with other pathways or proteins. It regulates the bioavailability and potential toxicity of different chemical substances in the body by modifying their structure and reactivity impacting pharmacokinetics significantly.

Pathways

NAT2 is deeply involved in xenobiotic metabolism and Phase II drug metabolism pathways. It contributes directly to the N-acetylation pathway critical for the biotransformation of pharmaceuticals. NAT2 works in tandem with Cytochrome P450 enzymes which usually perform initial oxidative modifications on compounds destined for NAT2 acetylation. This interaction highlights NAT2's role in creating metabolic balance by processing potential toxins and facilitating their removal or detoxification from the body.

NAT2 polymorphisms play a critical role in susceptibility to certain conditions such as bladder cancer and drug-induced lupus erythematosus. Slow acetylator phenotypes are associated with higher risk of bladder cancer due to inefficient detoxification of carcinogens. Furthermore NAT2 interplays with proteins like N-acetyltransferase 1 (NAT1) influencing the biological responses and effectiveness of therapeutic strategies. Understanding the role of NAT2 in these disorders is essential for developing personalized medicine approaches to treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates (PubMed : 12222688, PubMed : 7915226). Participates in the detoxification of a plethora of hydrazine and arylamine drugs, and is able to bioactivate several known carcinogens.
See full target information NAT2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com